Experiment: Cytosine 0.9 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS00980 and A4249_RS00985 are separated by 232 nucleotides A4249_RS00985 and A4249_RS00990 are separated by 72 nucleotides A4249_RS00990 and A4249_RS00995 are separated by 145 nucleotides
A4249_RS00980: A4249_RS00980 - pyruvate, phosphate dikinase, at 183,702 to 186,383
_RS00980
A4249_RS00985: A4249_RS00985 - porin family protein, at 186,616 to 187,143
_RS00985
A4249_RS00990: A4249_RS00990 - hypothetical protein, at 187,216 to 187,476
_RS00990
A4249_RS00995: A4249_RS00995 - M48 family metallopeptidase, at 187,622 to 188,827
_RS00995
Position (kb) 187
188 Strain fitness (log2 ratio) -2
-1
0
1
2 at 186.294 kb on + strand at 186.294 kb on + strand at 186.294 kb on + strand at 186.294 kb on + strand at 186.294 kb on + strand at 186.294 kb on + strand at 186.294 kb on + strand at 186.295 kb on - strand at 186.295 kb on - strand at 186.295 kb on - strand at 186.295 kb on - strand at 186.295 kb on - strand at 186.295 kb on - strand at 186.295 kb on - strand at 186.295 kb on - strand at 186.295 kb on - strand at 186.295 kb on - strand at 186.295 kb on - strand at 186.391 kb on + strand at 186.391 kb on + strand at 186.391 kb on + strand at 186.392 kb on - strand at 186.392 kb on - strand at 186.392 kb on - strand at 186.392 kb on - strand at 186.392 kb on - strand at 186.392 kb on - strand at 186.392 kb on - strand at 186.392 kb on - strand at 186.481 kb on - strand at 186.481 kb on - strand at 186.551 kb on + strand at 186.607 kb on + strand at 186.787 kb on + strand, within A4249_RS00985 at 186.787 kb on + strand, within A4249_RS00985 at 186.787 kb on + strand, within A4249_RS00985 at 186.787 kb on + strand, within A4249_RS00985 at 186.788 kb on - strand, within A4249_RS00985 at 186.788 kb on - strand, within A4249_RS00985 at 186.788 kb on - strand, within A4249_RS00985 at 186.788 kb on - strand, within A4249_RS00985 at 186.788 kb on - strand, within A4249_RS00985 at 186.889 kb on + strand, within A4249_RS00985 at 186.890 kb on - strand, within A4249_RS00985 at 186.890 kb on - strand, within A4249_RS00985 at 187.016 kb on - strand, within A4249_RS00985 at 187.016 kb on - strand, within A4249_RS00985 at 187.016 kb on - strand, within A4249_RS00985 at 187.033 kb on + strand, within A4249_RS00985 at 187.033 kb on + strand, within A4249_RS00985 at 187.033 kb on + strand, within A4249_RS00985 at 187.033 kb on + strand, within A4249_RS00985 at 187.033 kb on + strand, within A4249_RS00985 at 187.033 kb on + strand, within A4249_RS00985 at 187.033 kb on + strand, within A4249_RS00985 at 187.033 kb on + strand, within A4249_RS00985 at 187.033 kb on + strand, within A4249_RS00985 at 187.033 kb on + strand, within A4249_RS00985 at 187.034 kb on - strand, within A4249_RS00985 at 187.034 kb on - strand, within A4249_RS00985 at 187.034 kb on - strand, within A4249_RS00985 at 187.034 kb on - strand, within A4249_RS00985 at 187.034 kb on - strand, within A4249_RS00985 at 187.034 kb on - strand, within A4249_RS00985 at 187.034 kb on - strand, within A4249_RS00985 at 187.034 kb on - strand, within A4249_RS00985 at 187.034 kb on - strand, within A4249_RS00985 at 187.034 kb on - strand, within A4249_RS00985 at 187.034 kb on - strand, within A4249_RS00985 at 187.037 kb on - strand, within A4249_RS00985 at 187.037 kb on - strand, within A4249_RS00985 at 187.114 kb on + strand at 187.114 kb on + strand at 187.115 kb on - strand at 187.115 kb on - strand at 187.126 kb on + strand at 187.126 kb on + strand at 187.126 kb on + strand at 187.126 kb on + strand at 187.126 kb on + strand at 187.127 kb on - strand at 187.127 kb on - strand at 187.127 kb on - strand at 187.127 kb on - strand at 187.127 kb on - strand at 187.127 kb on - strand at 187.127 kb on - strand at 187.161 kb on - strand at 187.287 kb on - strand, within A4249_RS00990 at 187.289 kb on + strand, within A4249_RS00990 at 187.289 kb on + strand, within A4249_RS00990 at 187.289 kb on + strand, within A4249_RS00990 at 187.289 kb on + strand, within A4249_RS00990 at 187.289 kb on + strand, within A4249_RS00990 at 187.290 kb on - strand, within A4249_RS00990 at 187.290 kb on - strand, within A4249_RS00990 at 187.428 kb on + strand, within A4249_RS00990 at 187.571 kb on - strand at 187.580 kb on + strand at 187.703 kb on + strand at 187.703 kb on + strand at 187.703 kb on + strand at 187.703 kb on + strand at 187.704 kb on - strand at 187.704 kb on - strand at 187.704 kb on - strand at 187.704 kb on - strand at 187.704 kb on - strand at 187.705 kb on + strand at 187.706 kb on - strand at 187.706 kb on - strand at 187.706 kb on - strand at 187.862 kb on + strand, within A4249_RS00995 at 187.862 kb on + strand, within A4249_RS00995 at 187.863 kb on - strand, within A4249_RS00995 at 187.904 kb on + strand, within A4249_RS00995 at 187.904 kb on + strand, within A4249_RS00995 at 187.904 kb on + strand, within A4249_RS00995 at 187.904 kb on + strand, within A4249_RS00995 at 187.905 kb on - strand, within A4249_RS00995 at 187.905 kb on - strand, within A4249_RS00995 at 187.905 kb on - strand, within A4249_RS00995 at 187.905 kb on - strand, within A4249_RS00995 at 187.905 kb on - strand, within A4249_RS00995 at 187.931 kb on + strand, within A4249_RS00995 at 187.931 kb on + strand, within A4249_RS00995 at 187.932 kb on - strand, within A4249_RS00995 at 187.932 kb on - strand, within A4249_RS00995 at 187.932 kb on - strand, within A4249_RS00995 at 187.932 kb on - strand, within A4249_RS00995 at 187.932 kb on - strand, within A4249_RS00995 at 187.932 kb on - strand, within A4249_RS00995 at 187.932 kb on - strand, within A4249_RS00995 at 187.992 kb on + strand, within A4249_RS00995 at 188.030 kb on + strand, within A4249_RS00995 at 188.030 kb on + strand, within A4249_RS00995 at 188.030 kb on + strand, within A4249_RS00995 at 188.030 kb on + strand, within A4249_RS00995 at 188.030 kb on + strand, within A4249_RS00995 at 188.030 kb on + strand, within A4249_RS00995 at 188.030 kb on + strand, within A4249_RS00995 at 188.030 kb on + strand, within A4249_RS00995 at 188.031 kb on - strand, within A4249_RS00995 at 188.031 kb on - strand, within A4249_RS00995 at 188.031 kb on - strand, within A4249_RS00995 at 188.031 kb on - strand, within A4249_RS00995 at 188.031 kb on - strand, within A4249_RS00995 at 188.031 kb on - strand, within A4249_RS00995 at 188.031 kb on - strand, within A4249_RS00995 at 188.031 kb on - strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.150 kb on + strand, within A4249_RS00995 at 188.151 kb on - strand, within A4249_RS00995 at 188.151 kb on - strand, within A4249_RS00995 at 188.151 kb on - strand, within A4249_RS00995 at 188.151 kb on - strand, within A4249_RS00995 at 188.151 kb on - strand, within A4249_RS00995 at 188.151 kb on - strand, within A4249_RS00995 at 188.151 kb on - strand, within A4249_RS00995 at 188.151 kb on - strand, within A4249_RS00995 at 188.151 kb on - strand, within A4249_RS00995 at 188.151 kb on - strand, within A4249_RS00995 at 188.151 kb on - strand, within A4249_RS00995 at 188.151 kb on - strand, within A4249_RS00995 at 188.152 kb on + strand, within A4249_RS00995 at 188.152 kb on + strand, within A4249_RS00995 at 188.152 kb on + strand, within A4249_RS00995 at 188.237 kb on + strand, within A4249_RS00995 at 188.237 kb on + strand, within A4249_RS00995 at 188.237 kb on + strand, within A4249_RS00995 at 188.237 kb on + strand, within A4249_RS00995 at 188.238 kb on - strand, within A4249_RS00995 at 188.238 kb on - strand, within A4249_RS00995 at 188.238 kb on - strand, within A4249_RS00995 at 188.238 kb on - strand, within A4249_RS00995 at 188.238 kb on - strand, within A4249_RS00995 at 188.265 kb on - strand, within A4249_RS00995 at 188.294 kb on - strand, within A4249_RS00995 at 188.384 kb on + strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.466 kb on - strand, within A4249_RS00995
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine 0.9 mM (C) remove 186,294 + +0.6 186,294 + +0.6 186,294 + +0.2 186,294 + +0.2 186,294 + +0.2 186,294 + -0.7 186,294 + +0.5 186,295 - +0.1 186,295 - +0.0 186,295 - +0.5 186,295 - +0.2 186,295 - +1.0 186,295 - -0.1 186,295 - -0.0 186,295 - +0.2 186,295 - +0.4 186,295 - +0.5 186,295 - -0.1 186,391 + +0.1 186,391 + +0.1 186,391 + -0.2 186,392 - -0.0 186,392 - +0.2 186,392 - +0.7 186,392 - +1.3 186,392 - +0.2 186,392 - -0.3 186,392 - +0.3 186,392 - -0.4 186,481 - +0.3 186,481 - +0.2 186,551 + -0.0 186,607 + -1.1 186,787 + A4249_RS00985 0.32 -0.0 186,787 + A4249_RS00985 0.32 -0.2 186,787 + A4249_RS00985 0.32 +0.0 186,787 + A4249_RS00985 0.32 -1.1 186,788 - A4249_RS00985 0.33 -0.4 186,788 - A4249_RS00985 0.33 -0.3 186,788 - A4249_RS00985 0.33 -0.3 186,788 - A4249_RS00985 0.33 +0.4 186,788 - A4249_RS00985 0.33 -0.7 186,889 + A4249_RS00985 0.52 +0.2 186,890 - A4249_RS00985 0.52 +0.1 186,890 - A4249_RS00985 0.52 -0.1 187,016 - A4249_RS00985 0.76 +0.5 187,016 - A4249_RS00985 0.76 -0.1 187,016 - A4249_RS00985 0.76 +0.9 187,033 + A4249_RS00985 0.79 -0.2 187,033 + A4249_RS00985 0.79 -0.1 187,033 + A4249_RS00985 0.79 -0.8 187,033 + A4249_RS00985 0.79 +0.3 187,033 + A4249_RS00985 0.79 +0.2 187,033 + A4249_RS00985 0.79 -0.8 187,033 + A4249_RS00985 0.79 -0.5 187,033 + A4249_RS00985 0.79 -0.4 187,033 + A4249_RS00985 0.79 +0.5 187,033 + A4249_RS00985 0.79 +0.5 187,034 - A4249_RS00985 0.79 -1.3 187,034 - A4249_RS00985 0.79 -0.0 187,034 - A4249_RS00985 0.79 -0.1 187,034 - A4249_RS00985 0.79 -0.9 187,034 - A4249_RS00985 0.79 +0.3 187,034 - A4249_RS00985 0.79 -0.2 187,034 - A4249_RS00985 0.79 +0.5 187,034 - A4249_RS00985 0.79 +0.0 187,034 - A4249_RS00985 0.79 -0.6 187,034 - A4249_RS00985 0.79 -0.4 187,034 - A4249_RS00985 0.79 +0.2 187,037 - A4249_RS00985 0.80 -1.0 187,037 - A4249_RS00985 0.80 -1.2 187,114 + -0.1 187,114 + -0.4 187,115 - +0.7 187,115 - -0.7 187,126 + +0.1 187,126 + -0.2 187,126 + -0.2 187,126 + +0.2 187,126 + +0.0 187,127 - +1.2 187,127 - +0.6 187,127 - -1.7 187,127 - +0.1 187,127 - -0.4 187,127 - +0.1 187,127 - -0.9 187,161 - +0.4 187,287 - A4249_RS00990 0.27 -0.6 187,289 + A4249_RS00990 0.28 +0.0 187,289 + A4249_RS00990 0.28 +0.0 187,289 + A4249_RS00990 0.28 -0.2 187,289 + A4249_RS00990 0.28 -1.5 187,289 + A4249_RS00990 0.28 -0.1 187,290 - A4249_RS00990 0.28 -0.3 187,290 - A4249_RS00990 0.28 -0.2 187,428 + A4249_RS00990 0.81 -0.2 187,571 - +0.1 187,580 + +0.2 187,703 + -0.0 187,703 + -0.1 187,703 + +0.3 187,703 + +0.1 187,704 - -0.2 187,704 - -0.3 187,704 - -0.5 187,704 - -0.4 187,704 - +0.9 187,705 + +0.2 187,706 - -1.7 187,706 - -0.7 187,706 - +0.6 187,862 + A4249_RS00995 0.20 +0.0 187,862 + A4249_RS00995 0.20 -0.7 187,863 - A4249_RS00995 0.20 -0.0 187,904 + A4249_RS00995 0.23 -0.1 187,904 + A4249_RS00995 0.23 -0.1 187,904 + A4249_RS00995 0.23 -0.2 187,904 + A4249_RS00995 0.23 +0.8 187,905 - A4249_RS00995 0.23 +1.0 187,905 - A4249_RS00995 0.23 -0.2 187,905 - A4249_RS00995 0.23 -0.8 187,905 - A4249_RS00995 0.23 +1.2 187,905 - A4249_RS00995 0.23 -0.2 187,931 + A4249_RS00995 0.26 -0.6 187,931 + A4249_RS00995 0.26 -0.7 187,932 - A4249_RS00995 0.26 -0.4 187,932 - A4249_RS00995 0.26 -0.1 187,932 - A4249_RS00995 0.26 -0.1 187,932 - A4249_RS00995 0.26 -1.1 187,932 - A4249_RS00995 0.26 -1.5 187,932 - A4249_RS00995 0.26 -0.4 187,932 - A4249_RS00995 0.26 +0.1 187,992 + A4249_RS00995 0.31 +0.1 188,030 + A4249_RS00995 0.34 +0.0 188,030 + A4249_RS00995 0.34 -0.1 188,030 + A4249_RS00995 0.34 -0.0 188,030 + A4249_RS00995 0.34 +0.5 188,030 + A4249_RS00995 0.34 -0.3 188,030 + A4249_RS00995 0.34 -0.2 188,030 + A4249_RS00995 0.34 -1.3 188,030 + A4249_RS00995 0.34 +0.4 188,031 - A4249_RS00995 0.34 +0.6 188,031 - A4249_RS00995 0.34 -0.1 188,031 - A4249_RS00995 0.34 -1.3 188,031 - A4249_RS00995 0.34 -0.4 188,031 - A4249_RS00995 0.34 -0.4 188,031 - A4249_RS00995 0.34 +0.6 188,031 - A4249_RS00995 0.34 +0.2 188,031 - A4249_RS00995 0.34 -1.0 188,150 + A4249_RS00995 0.44 +1.8 188,150 + A4249_RS00995 0.44 +0.5 188,150 + A4249_RS00995 0.44 -2.1 188,150 + A4249_RS00995 0.44 -1.1 188,150 + A4249_RS00995 0.44 +0.3 188,150 + A4249_RS00995 0.44 -0.4 188,150 + A4249_RS00995 0.44 +0.0 188,150 + A4249_RS00995 0.44 -0.1 188,150 + A4249_RS00995 0.44 +0.0 188,150 + A4249_RS00995 0.44 +0.0 188,150 + A4249_RS00995 0.44 -0.0 188,150 + A4249_RS00995 0.44 +0.7 188,150 + A4249_RS00995 0.44 -0.4 188,150 + A4249_RS00995 0.44 +0.1 188,150 + A4249_RS00995 0.44 -0.6 188,150 + A4249_RS00995 0.44 +0.3 188,150 + A4249_RS00995 0.44 -0.6 188,150 + A4249_RS00995 0.44 +0.2 188,150 + A4249_RS00995 0.44 +0.0 188,151 - A4249_RS00995 0.44 +0.5 188,151 - A4249_RS00995 0.44 +0.4 188,151 - A4249_RS00995 0.44 -0.9 188,151 - A4249_RS00995 0.44 +0.3 188,151 - A4249_RS00995 0.44 +0.4 188,151 - A4249_RS00995 0.44 -0.7 188,151 - A4249_RS00995 0.44 +0.5 188,151 - A4249_RS00995 0.44 -0.2 188,151 - A4249_RS00995 0.44 -1.4 188,151 - A4249_RS00995 0.44 -0.9 188,151 - A4249_RS00995 0.44 +0.2 188,151 - A4249_RS00995 0.44 -0.4 188,152 + A4249_RS00995 0.44 +0.5 188,152 + A4249_RS00995 0.44 -0.2 188,152 + A4249_RS00995 0.44 -0.3 188,237 + A4249_RS00995 0.51 -0.5 188,237 + A4249_RS00995 0.51 +0.6 188,237 + A4249_RS00995 0.51 +0.6 188,237 + A4249_RS00995 0.51 +1.2 188,238 - A4249_RS00995 0.51 -0.1 188,238 - A4249_RS00995 0.51 +0.1 188,238 - A4249_RS00995 0.51 -0.5 188,238 - A4249_RS00995 0.51 -0.1 188,238 - A4249_RS00995 0.51 +0.7 188,265 - A4249_RS00995 0.53 +1.1 188,294 - A4249_RS00995 0.56 -0.7 188,384 + A4249_RS00995 0.63 -0.2 188,385 - A4249_RS00995 0.63 +0.2 188,385 - A4249_RS00995 0.63 +0.2 188,385 - A4249_RS00995 0.63 -0.8 188,385 - A4249_RS00995 0.63 -0.3 188,385 - A4249_RS00995 0.63 -0.4 188,385 - A4249_RS00995 0.63 +0.8 188,385 - A4249_RS00995 0.63 -2.0 188,385 - A4249_RS00995 0.63 -0.1 188,466 - A4249_RS00995 0.70 +0.5
Or see this region's nucleotide sequence