Experiment: Bas38
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfB and hyfA overlap by 1 nucleotides hyfA and bcp are separated by 252 nucleotides bcp and gcvR overlap by 1 nucleotides
HEPCGN_21065: hyfB - hydrogenase 4 subunit B, at 2,965,027 to 2,967,045
hyfB
HEPCGN_21070: hyfA - hydrogenase 4 subunit HyfA, at 2,967,045 to 2,967,662
hyfA
HEPCGN_21075: bcp - thioredoxin-dependent thiol peroxidase, at 2,967,915 to 2,968,385
bcp
HEPCGN_21080: gcvR - glycine cleavage system transcriptional repressor, at 2,968,385 to 2,968,957
gcvR
Position (kb)
2967
2968 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2966.148 kb on + strand, within hyfB at 2966.283 kb on + strand, within hyfB at 2966.511 kb on + strand, within hyfB at 2966.511 kb on + strand, within hyfB at 2966.512 kb on - strand, within hyfB at 2966.512 kb on - strand, within hyfB at 2966.538 kb on + strand, within hyfB at 2966.574 kb on + strand, within hyfB at 2966.575 kb on - strand, within hyfB at 2966.695 kb on - strand, within hyfB at 2966.739 kb on + strand, within hyfB at 2966.740 kb on - strand, within hyfB at 2966.741 kb on + strand, within hyfB at 2966.741 kb on + strand, within hyfB at 2966.742 kb on - strand, within hyfB at 2966.742 kb on - strand, within hyfB at 2966.753 kb on + strand, within hyfB at 2966.785 kb on - strand, within hyfB at 2966.797 kb on - strand, within hyfB at 2966.884 kb on + strand at 2966.884 kb on + strand at 2966.885 kb on - strand at 2966.885 kb on - strand at 2966.885 kb on - strand at 2966.893 kb on - strand at 2966.953 kb on - strand at 2967.026 kb on - strand at 2967.028 kb on + strand at 2967.029 kb on - strand at 2967.417 kb on - strand, within hyfA at 2967.571 kb on - strand, within hyfA at 2967.584 kb on + strand, within hyfA at 2967.585 kb on - strand, within hyfA at 2967.614 kb on + strand at 2967.614 kb on + strand at 2967.615 kb on - strand at 2967.617 kb on + strand at 2967.617 kb on + strand at 2967.617 kb on + strand at 2967.617 kb on + strand at 2967.617 kb on + strand at 2967.618 kb on - strand at 2967.618 kb on - strand at 2967.626 kb on + strand at 2967.626 kb on + strand at 2967.912 kb on + strand at 2968.031 kb on + strand, within bcp at 2968.039 kb on + strand, within bcp at 2968.124 kb on + strand, within bcp at 2968.166 kb on + strand, within bcp at 2968.167 kb on - strand, within bcp at 2968.221 kb on - strand, within bcp at 2968.242 kb on + strand, within bcp at 2968.242 kb on + strand, within bcp at 2968.243 kb on - strand, within bcp at 2968.317 kb on - strand, within bcp at 2968.317 kb on - strand, within bcp at 2968.386 kb on + strand at 2968.386 kb on + strand at 2968.387 kb on - strand at 2968.390 kb on + strand at 2968.391 kb on - strand at 2968.391 kb on - strand at 2968.391 kb on - strand at 2968.463 kb on + strand, within gcvR at 2968.463 kb on + strand, within gcvR at 2968.464 kb on - strand, within gcvR at 2968.464 kb on - strand, within gcvR at 2968.486 kb on + strand, within gcvR at 2968.523 kb on + strand, within gcvR at 2968.597 kb on + strand, within gcvR at 2968.597 kb on + strand, within gcvR
Per-strain Table
Position Strand Gene LocusTag Fraction Bas38 remove 2,966,148 + hyfB HEPCGN_21065 0.56 +0.4 2,966,283 + hyfB HEPCGN_21065 0.62 -0.1 2,966,511 + hyfB HEPCGN_21065 0.74 -0.4 2,966,511 + hyfB HEPCGN_21065 0.74 +0.4 2,966,512 - hyfB HEPCGN_21065 0.74 -0.2 2,966,512 - hyfB HEPCGN_21065 0.74 +0.5 2,966,538 + hyfB HEPCGN_21065 0.75 -0.3 2,966,574 + hyfB HEPCGN_21065 0.77 -0.2 2,966,575 - hyfB HEPCGN_21065 0.77 +0.2 2,966,695 - hyfB HEPCGN_21065 0.83 -0.5 2,966,739 + hyfB HEPCGN_21065 0.85 -0.4 2,966,740 - hyfB HEPCGN_21065 0.85 -0.2 2,966,741 + hyfB HEPCGN_21065 0.85 -0.0 2,966,741 + hyfB HEPCGN_21065 0.85 -0.0 2,966,742 - hyfB HEPCGN_21065 0.85 -0.3 2,966,742 - hyfB HEPCGN_21065 0.85 -0.1 2,966,753 + hyfB HEPCGN_21065 0.85 -0.2 2,966,785 - hyfB HEPCGN_21065 0.87 -0.4 2,966,797 - hyfB HEPCGN_21065 0.88 +0.3 2,966,884 + +0.5 2,966,884 + -0.4 2,966,885 - -0.4 2,966,885 - -0.0 2,966,885 - -0.2 2,966,893 - +0.6 2,966,953 - -0.5 2,967,026 - -0.6 2,967,028 + +0.0 2,967,029 - -0.2 2,967,417 - hyfA HEPCGN_21070 0.60 +0.1 2,967,571 - hyfA HEPCGN_21070 0.85 +0.4 2,967,584 + hyfA HEPCGN_21070 0.87 +0.3 2,967,585 - hyfA HEPCGN_21070 0.87 +0.7 2,967,614 + +1.0 2,967,614 + +0.0 2,967,615 - +0.5 2,967,617 + +0.0 2,967,617 + +1.8 2,967,617 + -1.8 2,967,617 + -0.4 2,967,617 + +0.0 2,967,618 - -0.2 2,967,618 - +0.4 2,967,626 + +1.2 2,967,626 + +0.3 2,967,912 + +0.4 2,968,031 + bcp HEPCGN_21075 0.25 +0.0 2,968,039 + bcp HEPCGN_21075 0.26 -0.2 2,968,124 + bcp HEPCGN_21075 0.44 +0.5 2,968,166 + bcp HEPCGN_21075 0.53 +0.8 2,968,167 - bcp HEPCGN_21075 0.54 -1.3 2,968,221 - bcp HEPCGN_21075 0.65 -0.7 2,968,242 + bcp HEPCGN_21075 0.69 +0.0 2,968,242 + bcp HEPCGN_21075 0.69 +0.3 2,968,243 - bcp HEPCGN_21075 0.70 +0.5 2,968,317 - bcp HEPCGN_21075 0.85 -0.5 2,968,317 - bcp HEPCGN_21075 0.85 +0.1 2,968,386 + -0.8 2,968,386 + +0.8 2,968,387 - +0.2 2,968,390 + -0.3 2,968,391 - +0.4 2,968,391 - -0.7 2,968,391 - +0.7 2,968,463 + gcvR HEPCGN_21080 0.14 +0.5 2,968,463 + gcvR HEPCGN_21080 0.14 +0.2 2,968,464 - gcvR HEPCGN_21080 0.14 +0.9 2,968,464 - gcvR HEPCGN_21080 0.14 -0.4 2,968,486 + gcvR HEPCGN_21080 0.18 +0.2 2,968,523 + gcvR HEPCGN_21080 0.24 +0.8 2,968,597 + gcvR HEPCGN_21080 0.37 +0.4 2,968,597 + gcvR HEPCGN_21080 0.37 -0.2
Or see this region's nucleotide sequence