Experiment: Bas38
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_11055 and HEPCGN_11060 are separated by 81 nucleotides HEPCGN_11060 and yeeS are separated by 11 nucleotides yeeS and HEPCGN_11070 are separated by 62 nucleotides HEPCGN_11070 and HEPCGN_11075 overlap by 1 nucleotides HEPCGN_11075 and cbtA are separated by 49 nucleotides cbtA and HEPCGN_11085 are separated by 89 nucleotides
HEPCGN_11055: HEPCGN_11055 - DUF945 domain-containing protein, at 950,654 to 951,472
_11055
HEPCGN_11060: HEPCGN_11060 - restriction endonuclease, at 951,554 to 952,033
_11060
HEPCGN_11065: yeeS - UPF0758 protein YeeS, at 952,045 to 952,521
yeeS
HEPCGN_11070: HEPCGN_11070 - DUF987 domain-containing protein, at 952,584 to 952,805
_11070
HEPCGN_11075: HEPCGN_11075 - Phage-related protein, at 952,805 to 952,918
_11075
HEPCGN_11080: cbtA - type IV toxin-antitoxin system toxin CbtA, at 952,968 to 953,336
cbtA
HEPCGN_11085: HEPCGN_11085 - toxin, at 953,426 to 953,800
_11085
Position (kb) 952
953 Strain fitness (log2 ratio) -1
0
1 at 951.061 kb on - strand, within HEPCGN_11055 at 951.293 kb on - strand, within HEPCGN_11055 at 951.536 kb on + strand at 951.800 kb on - strand, within HEPCGN_11060 at 951.948 kb on - strand, within HEPCGN_11060 at 951.948 kb on - strand, within HEPCGN_11060 at 952.411 kb on - strand, within yeeS at 952.668 kb on + strand, within HEPCGN_11070 at 952.695 kb on + strand, within HEPCGN_11070 at 952.695 kb on + strand, within HEPCGN_11070 at 952.696 kb on - strand, within HEPCGN_11070 at 952.696 kb on - strand, within HEPCGN_11070 at 952.696 kb on - strand, within HEPCGN_11070 at 952.773 kb on + strand, within HEPCGN_11070 at 952.875 kb on + strand, within HEPCGN_11075 at 952.931 kb on - strand at 952.964 kb on + strand at 953.095 kb on - strand, within cbtA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas38 remove 951,061 - HEPCGN_11055 0.50 -0.2 951,293 - HEPCGN_11055 0.78 +0.3 951,536 + -0.4 951,800 - HEPCGN_11060 0.51 -0.0 951,948 - HEPCGN_11060 0.82 -0.2 951,948 - HEPCGN_11060 0.82 -0.1 952,411 - yeeS HEPCGN_11065 0.77 -0.5 952,668 + HEPCGN_11070 0.38 -0.2 952,695 + HEPCGN_11070 0.50 -0.6 952,695 + HEPCGN_11070 0.50 -1.5 952,696 - HEPCGN_11070 0.50 +0.0 952,696 - HEPCGN_11070 0.50 -0.0 952,696 - HEPCGN_11070 0.50 -0.7 952,773 + HEPCGN_11070 0.85 +0.1 952,875 + HEPCGN_11075 0.61 +0.4 952,931 - -1.1 952,964 + -0.2 953,095 - cbtA HEPCGN_11080 0.34 +0.1
Or see this region's nucleotide sequence