Strain Fitness in Escherichia coli ECOR38 around HEPCGN_07425

Experiment: Bas38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlacI and lacZ are separated by 122 nucleotideslacZ and lacY are separated by 51 nucleotides HEPCGN_07420: lacI - DNA-binding transcriptional repressor LacI, at 218,360 to 219,451 lacI HEPCGN_07425: lacZ - beta-galactosidase, at 219,574 to 222,648 lacZ HEPCGN_07430: lacY - lactose permease, at 222,700 to 223,953 lacY Position (kb) 219 220 221 222 223Strain fitness (log2 ratio) -1 0 1 2at 218.618 kb on - strand, within lacIat 218.740 kb on + strand, within lacIat 218.745 kb on - strand, within lacIat 218.845 kb on + strand, within lacIat 218.922 kb on + strand, within lacIat 218.934 kb on + strand, within lacIat 218.974 kb on + strand, within lacIat 218.978 kb on + strand, within lacIat 219.212 kb on + strand, within lacIat 219.213 kb on - strand, within lacIat 219.486 kb on + strandat 219.567 kb on + strandat 219.574 kb on - strandat 219.574 kb on - strandat 219.649 kb on - strandat 219.688 kb on - strandat 220.158 kb on + strand, within lacZat 220.179 kb on + strand, within lacZat 220.179 kb on + strand, within lacZat 220.180 kb on - strand, within lacZat 220.180 kb on - strand, within lacZat 220.225 kb on - strand, within lacZat 220.269 kb on + strand, within lacZat 220.269 kb on + strand, within lacZat 220.269 kb on + strand, within lacZat 220.315 kb on + strand, within lacZat 220.771 kb on + strand, within lacZat 220.772 kb on - strand, within lacZat 220.779 kb on - strand, within lacZat 220.798 kb on + strand, within lacZat 220.799 kb on - strand, within lacZat 220.921 kb on - strand, within lacZat 220.934 kb on + strand, within lacZat 220.935 kb on - strand, within lacZat 221.070 kb on + strand, within lacZat 221.189 kb on - strand, within lacZat 221.231 kb on - strand, within lacZat 221.261 kb on + strand, within lacZat 221.305 kb on - strand, within lacZat 221.309 kb on - strand, within lacZat 221.631 kb on - strand, within lacZat 221.675 kb on + strand, within lacZat 221.676 kb on - strand, within lacZat 221.676 kb on - strand, within lacZat 221.835 kb on + strand, within lacZat 221.985 kb on + strand, within lacZat 222.060 kb on + strand, within lacZat 222.060 kb on + strand, within lacZat 222.061 kb on - strand, within lacZat 222.061 kb on - strand, within lacZat 222.085 kb on - strand, within lacZat 222.126 kb on + strand, within lacZat 222.126 kb on + strand, within lacZat 222.156 kb on + strand, within lacZat 222.191 kb on + strand, within lacZat 222.275 kb on + strand, within lacZat 222.352 kb on + strandat 222.352 kb on + strandat 222.352 kb on + strandat 222.353 kb on - strandat 222.353 kb on - strandat 222.353 kb on - strandat 222.353 kb on - strandat 222.353 kb on - strandat 222.353 kb on - strandat 222.361 kb on + strandat 222.361 kb on + strandat 222.412 kb on + strandat 222.412 kb on + strandat 222.617 kb on - strandat 222.649 kb on + strandat 223.083 kb on + strand, within lacYat 223.195 kb on - strand, within lacYat 223.323 kb on + strand, within lacYat 223.381 kb on + strand, within lacYat 223.381 kb on + strand, within lacYat 223.381 kb on + strand, within lacYat 223.382 kb on - strand, within lacYat 223.382 kb on - strand, within lacYat 223.382 kb on - strand, within lacYat 223.382 kb on - strand, within lacYat 223.405 kb on + strand, within lacYat 223.406 kb on - strand, within lacYat 223.406 kb on - strand, within lacYat 223.406 kb on - strand, within lacYat 223.513 kb on - strand, within lacYat 223.525 kb on - strand, within lacYat 223.593 kb on + strand, within lacYat 223.594 kb on - strand, within lacYat 223.601 kb on + strand, within lacYat 223.606 kb on - strand, within lacY

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas38
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218,618 - lacI HEPCGN_07420 0.24 -0.2
218,740 + lacI HEPCGN_07420 0.35 -0.1
218,745 - lacI HEPCGN_07420 0.35 -1.6
218,845 + lacI HEPCGN_07420 0.44 +0.9
218,922 + lacI HEPCGN_07420 0.51 -1.1
218,934 + lacI HEPCGN_07420 0.53 -0.4
218,974 + lacI HEPCGN_07420 0.56 -0.5
218,978 + lacI HEPCGN_07420 0.57 -0.5
219,212 + lacI HEPCGN_07420 0.78 -0.1
219,213 - lacI HEPCGN_07420 0.78 -0.0
219,486 + +0.4
219,567 + -0.9
219,574 - -0.1
219,574 - -0.1
219,649 - +0.1
219,688 - +1.1
220,158 + lacZ HEPCGN_07425 0.19 +0.1
220,179 + lacZ HEPCGN_07425 0.20 +0.2
220,179 + lacZ HEPCGN_07425 0.20 -1.3
220,180 - lacZ HEPCGN_07425 0.20 +0.2
220,180 - lacZ HEPCGN_07425 0.20 +0.3
220,225 - lacZ HEPCGN_07425 0.21 -0.1
220,269 + lacZ HEPCGN_07425 0.23 +0.4
220,269 + lacZ HEPCGN_07425 0.23 -0.5
220,269 + lacZ HEPCGN_07425 0.23 -0.0
220,315 + lacZ HEPCGN_07425 0.24 -0.2
220,771 + lacZ HEPCGN_07425 0.39 +2.3
220,772 - lacZ HEPCGN_07425 0.39 +0.2
220,779 - lacZ HEPCGN_07425 0.39 -0.2
220,798 + lacZ HEPCGN_07425 0.40 -0.6
220,799 - lacZ HEPCGN_07425 0.40 -0.1
220,921 - lacZ HEPCGN_07425 0.44 +0.1
220,934 + lacZ HEPCGN_07425 0.44 -0.3
220,935 - lacZ HEPCGN_07425 0.44 +0.4
221,070 + lacZ HEPCGN_07425 0.49 -0.3
221,189 - lacZ HEPCGN_07425 0.53 +0.3
221,231 - lacZ HEPCGN_07425 0.54 +0.2
221,261 + lacZ HEPCGN_07425 0.55 -0.6
221,305 - lacZ HEPCGN_07425 0.56 +0.3
221,309 - lacZ HEPCGN_07425 0.56 +0.3
221,631 - lacZ HEPCGN_07425 0.67 +0.3
221,675 + lacZ HEPCGN_07425 0.68 +0.8
221,676 - lacZ HEPCGN_07425 0.68 -0.2
221,676 - lacZ HEPCGN_07425 0.68 +0.1
221,835 + lacZ HEPCGN_07425 0.74 +0.2
221,985 + lacZ HEPCGN_07425 0.78 +0.3
222,060 + lacZ HEPCGN_07425 0.81 +0.1
222,060 + lacZ HEPCGN_07425 0.81 +0.2
222,061 - lacZ HEPCGN_07425 0.81 +0.5
222,061 - lacZ HEPCGN_07425 0.81 +0.0
222,085 - lacZ HEPCGN_07425 0.82 +0.4
222,126 + lacZ HEPCGN_07425 0.83 +0.1
222,126 + lacZ HEPCGN_07425 0.83 -1.3
222,156 + lacZ HEPCGN_07425 0.84 -1.7
222,191 + lacZ HEPCGN_07425 0.85 +0.0
222,275 + lacZ HEPCGN_07425 0.88 -0.0
222,352 + -0.8
222,352 + -0.6
222,352 + +0.1
222,353 - -0.3
222,353 - +0.3
222,353 - +0.3
222,353 - -0.3
222,353 - +1.0
222,353 - -0.0
222,361 + +0.8
222,361 + +0.2
222,412 + -0.3
222,412 + -1.1
222,617 - +0.2
222,649 + +0.2
223,083 + lacY HEPCGN_07430 0.31 +0.0
223,195 - lacY HEPCGN_07430 0.39 -0.4
223,323 + lacY HEPCGN_07430 0.50 -0.1
223,381 + lacY HEPCGN_07430 0.54 +0.6
223,381 + lacY HEPCGN_07430 0.54 +0.5
223,381 + lacY HEPCGN_07430 0.54 -0.7
223,382 - lacY HEPCGN_07430 0.54 -0.1
223,382 - lacY HEPCGN_07430 0.54 -0.1
223,382 - lacY HEPCGN_07430 0.54 -0.2
223,382 - lacY HEPCGN_07430 0.54 +0.5
223,405 + lacY HEPCGN_07430 0.56 +0.2
223,406 - lacY HEPCGN_07430 0.56 +0.0
223,406 - lacY HEPCGN_07430 0.56 +0.8
223,406 - lacY HEPCGN_07430 0.56 +0.2
223,513 - lacY HEPCGN_07430 0.65 +0.6
223,525 - lacY HEPCGN_07430 0.66 -0.2
223,593 + lacY HEPCGN_07430 0.71 -0.7
223,594 - lacY HEPCGN_07430 0.71 -0.3
223,601 + lacY HEPCGN_07430 0.72 -0.7
223,606 - lacY HEPCGN_07430 0.72 -0.2

Or see this region's nucleotide sequence