Experiment: Bas38
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnp-ISSd1 and tnp-IS600 are separated by 35 nucleotides tnp-IS600 and HEPCGN_06310 are separated by 130 nucleotides HEPCGN_06310 and istB are separated by 43 nucleotides istB and istA overlap by 1 nucleotides
HEPCGN_06300: tnp-ISSd1 - IS3 family ISSd1 transposase, at 1,127,816 to 1,128,118
tnp-ISSd1
HEPCGN_06305: tnp-IS600 - IS3 family IS600 transposase, at 1,128,154 to 1,128,972
tnp-IS600
HEPCGN_06310: HEPCGN_06310 - antitermination protein, at 1,129,103 to 1,129,531
_06310
HEPCGN_06315: istB - IS21-like element IS100kyp family helper ATPase IstB, at 1,129,575 to 1,130,354
istB
HEPCGN_06320: istA - IS21-like element IS100 family transposase, at 1,130,354 to 1,131,376
istA
Position (kb)
1129
1130 Strain fitness (log2 ratio)
-1
0
1 at 1129.026 kb on - strand at 1129.087 kb on - strand at 1129.093 kb on + strand at 1129.206 kb on + strand, within HEPCGN_06310 at 1129.307 kb on + strand, within HEPCGN_06310 at 1129.327 kb on + strand, within HEPCGN_06310 at 1129.364 kb on + strand, within HEPCGN_06310 at 1129.365 kb on - strand, within HEPCGN_06310 at 1129.367 kb on - strand, within HEPCGN_06310 at 1129.367 kb on - strand, within HEPCGN_06310 at 1129.367 kb on - strand, within HEPCGN_06310 at 1129.371 kb on + strand, within HEPCGN_06310 at 1129.440 kb on + strand, within HEPCGN_06310 at 1129.492 kb on + strand at 1129.493 kb on - strand at 1129.539 kb on - strand at 1129.539 kb on - strand at 1130.291 kb on + strand at 1130.382 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas38 remove 1,129,026 - -0.2 1,129,087 - +0.3 1,129,093 + +0.6 1,129,206 + HEPCGN_06310 0.24 -0.7 1,129,307 + HEPCGN_06310 0.48 +0.4 1,129,327 + HEPCGN_06310 0.52 +0.2 1,129,364 + HEPCGN_06310 0.61 -0.6 1,129,365 - HEPCGN_06310 0.61 -0.7 1,129,367 - HEPCGN_06310 0.62 -0.5 1,129,367 - HEPCGN_06310 0.62 -1.0 1,129,367 - HEPCGN_06310 0.62 -0.9 1,129,371 + HEPCGN_06310 0.62 +0.2 1,129,440 + HEPCGN_06310 0.79 +0.2 1,129,492 + +1.6 1,129,493 - -0.3 1,129,539 - -1.7 1,129,539 - +0.1 1,130,291 + -0.1 1,130,382 + -0.4
Or see this region's nucleotide sequence