Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ychO and ychN are separated by 149 nucleotides ychN and chaC are separated by 43 nucleotides chaC and chaB are separated by 136 nucleotides chaB and chaA are separated by 269 nucleotides
NIAGMN_27720: ychO - inverse autotransporter invasin YchO, at 5,373,266 to 5,374,696
ychO
NIAGMN_27725: ychN - DsrE/F sulfur relay family protein YchN, at 5,374,846 to 5,375,199
ychN
NIAGMN_27730: chaC - glutathione-specific gamma-glutamylcyclotransferase, at 5,375,243 to 5,375,962
chaC
NIAGMN_27735: chaB - putative cation transport regulator ChaB, at 5,376,099 to 5,376,329
chaB
NIAGMN_27740: chaA - sodium-potassium/proton antiporter ChaA, at 5,376,599 to 5,377,699
chaA
Position (kb)
5375
5376 Strain fitness (log2 ratio)
-1
0
1
2
3 at 5374.546 kb on + strand, within ychO at 5374.549 kb on + strand, within ychO at 5374.582 kb on + strand at 5375.867 kb on + strand, within chaC at 5375.944 kb on + strand at 5376.083 kb on - strand at 5376.215 kb on + strand, within chaB at 5376.222 kb on - strand, within chaB at 5376.224 kb on - strand, within chaB at 5376.706 kb on - strand at 5376.829 kb on + strand, within chaA at 5376.913 kb on + strand, within chaA
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 5,374,546 + ychO NIAGMN_27720 0.89 +2.3 5,374,549 + ychO NIAGMN_27720 0.90 -0.4 5,374,582 + +3.2 5,375,867 + chaC NIAGMN_27730 0.87 -1.5 5,375,944 + -0.2 5,376,083 - -0.3 5,376,215 + chaB NIAGMN_27735 0.50 +1.5 5,376,222 - chaB NIAGMN_27735 0.53 +2.5 5,376,224 - chaB NIAGMN_27735 0.54 -0.4 5,376,706 - -0.3 5,376,829 + chaA NIAGMN_27740 0.21 +3.6 5,376,913 + chaA NIAGMN_27740 0.29 +1.2
Or see this region's nucleotide sequence