Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rutE and rutD are separated by 9 nucleotides rutD and rutC are separated by 7 nucleotides rutC and rutB are separated by 11 nucleotides rutB and rutA overlap by 4 nucleotides
NIAGMN_23700: rutE - malonic semialdehyde reductase, at 4,631,176 to 4,631,766
rutE
NIAGMN_23705: rutD - pyrimidine utilization protein D, at 4,631,776 to 4,632,576
rutD
NIAGMN_23710: rutC - pyrimidine utilization protein C, at 4,632,584 to 4,632,970
rutC
NIAGMN_23715: rutB - peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB, at 4,632,982 to 4,633,677
rutB
NIAGMN_23720: rutA - pyrimidine utilization protein A, at 4,633,674 to 4,634,765
rutA
Position (kb)
4632
4633 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4631.607 kb on + strand, within rutE at 4631.607 kb on + strand, within rutE at 4631.800 kb on + strand at 4631.801 kb on - strand at 4631.836 kb on - strand at 4632.180 kb on - strand, within rutD at 4632.395 kb on + strand, within rutD at 4632.452 kb on - strand, within rutD at 4632.452 kb on - strand, within rutD at 4632.596 kb on - strand at 4632.671 kb on - strand, within rutC at 4632.786 kb on - strand, within rutC at 4632.885 kb on + strand, within rutC at 4632.885 kb on + strand, within rutC at 4632.885 kb on + strand, within rutC at 4632.886 kb on - strand, within rutC at 4632.886 kb on - strand, within rutC at 4632.886 kb on - strand, within rutC at 4632.974 kb on + strand at 4633.139 kb on + strand, within rutB at 4633.268 kb on + strand, within rutB at 4633.377 kb on + strand, within rutB at 4633.442 kb on + strand, within rutB at 4633.568 kb on - strand, within rutB at 4633.571 kb on + strand, within rutB at 4633.572 kb on - strand, within rutB at 4633.590 kb on - strand, within rutB
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 4,631,607 + rutE NIAGMN_23700 0.73 +1.3 4,631,607 + rutE NIAGMN_23700 0.73 +3.7 4,631,800 + -0.2 4,631,801 - +0.5 4,631,836 - +0.1 4,632,180 - rutD NIAGMN_23705 0.50 -0.2 4,632,395 + rutD NIAGMN_23705 0.77 -1.3 4,632,452 - rutD NIAGMN_23705 0.84 -0.7 4,632,452 - rutD NIAGMN_23705 0.84 -0.4 4,632,596 - -1.2 4,632,671 - rutC NIAGMN_23710 0.22 +0.3 4,632,786 - rutC NIAGMN_23710 0.52 -0.1 4,632,885 + rutC NIAGMN_23710 0.78 -0.8 4,632,885 + rutC NIAGMN_23710 0.78 -1.9 4,632,885 + rutC NIAGMN_23710 0.78 -1.1 4,632,886 - rutC NIAGMN_23710 0.78 -0.7 4,632,886 - rutC NIAGMN_23710 0.78 -1.5 4,632,886 - rutC NIAGMN_23710 0.78 -3.6 4,632,974 + +0.4 4,633,139 + rutB NIAGMN_23715 0.23 +0.4 4,633,268 + rutB NIAGMN_23715 0.41 +2.8 4,633,377 + rutB NIAGMN_23715 0.57 -0.3 4,633,442 + rutB NIAGMN_23715 0.66 -0.3 4,633,568 - rutB NIAGMN_23715 0.84 +0.1 4,633,571 + rutB NIAGMN_23715 0.85 -0.8 4,633,572 - rutB NIAGMN_23715 0.85 +0.3 4,633,590 - rutB NIAGMN_23715 0.87 +1.3
Or see this region's nucleotide sequence