Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisD and hisG are separated by 5 nucleotides hisG and hisL are separated by 145 nucleotides hisL and yeeZ are separated by 425 nucleotides
NIAGMN_22460: hisD - Histidinol dehydrogenase, at 4,435,906 to 4,437,210
hisD
NIAGMN_22465: hisG - ATP phosphoribosyltransferase, at 4,437,216 to 4,438,115
hisG
NIAGMN_22470: hisL - his operon leader peptide, at 4,438,261 to 4,438,311
hisL
NIAGMN_22475: yeeZ - Protein YeeZ, at 4,438,737 to 4,439,561
yeeZ
Position (kb)
4437
4438
4439 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4436.382 kb on + strand, within hisD at 4436.823 kb on - strand, within hisD at 4436.823 kb on - strand, within hisD at 4436.959 kb on + strand, within hisD at 4436.960 kb on - strand, within hisD at 4437.049 kb on - strand, within hisD at 4437.049 kb on - strand, within hisD at 4437.174 kb on + strand at 4437.181 kb on - strand at 4437.434 kb on - strand, within hisG at 4437.551 kb on - strand, within hisG at 4437.554 kb on + strand, within hisG at 4437.704 kb on - strand, within hisG at 4438.086 kb on - strand at 4438.086 kb on - strand at 4438.089 kb on + strand at 4438.090 kb on - strand at 4438.389 kb on + strand at 4438.390 kb on - strand at 4438.393 kb on + strand at 4438.394 kb on - strand at 4438.444 kb on + strand at 4438.454 kb on + strand at 4438.638 kb on + strand at 4438.640 kb on + strand at 4438.640 kb on + strand at 4438.700 kb on + strand at 4438.706 kb on + strand at 4438.708 kb on + strand at 4438.731 kb on + strand at 4438.874 kb on + strand, within yeeZ at 4438.878 kb on + strand, within yeeZ at 4438.958 kb on + strand, within yeeZ at 4438.958 kb on + strand, within yeeZ at 4439.045 kb on + strand, within yeeZ
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 4,436,382 + hisD NIAGMN_22460 0.36 -1.0 4,436,823 - hisD NIAGMN_22460 0.70 -1.5 4,436,823 - hisD NIAGMN_22460 0.70 -0.8 4,436,959 + hisD NIAGMN_22460 0.81 -3.6 4,436,960 - hisD NIAGMN_22460 0.81 -0.5 4,437,049 - hisD NIAGMN_22460 0.88 -1.3 4,437,049 - hisD NIAGMN_22460 0.88 -2.4 4,437,174 + -3.5 4,437,181 - -0.8 4,437,434 - hisG NIAGMN_22465 0.24 -0.3 4,437,551 - hisG NIAGMN_22465 0.37 -4.3 4,437,554 + hisG NIAGMN_22465 0.38 -1.8 4,437,704 - hisG NIAGMN_22465 0.54 -2.7 4,438,086 - -1.7 4,438,086 - -3.2 4,438,089 + -1.5 4,438,090 - -2.8 4,438,389 + -1.2 4,438,390 - -2.2 4,438,393 + +0.3 4,438,394 - -2.0 4,438,444 + +2.7 4,438,454 + +0.0 4,438,638 + -0.9 4,438,640 + -0.9 4,438,640 + -0.3 4,438,700 + +0.7 4,438,706 + +0.2 4,438,708 + -1.6 4,438,731 + -0.5 4,438,874 + yeeZ NIAGMN_22475 0.17 -0.3 4,438,878 + yeeZ NIAGMN_22475 0.17 -2.0 4,438,958 + yeeZ NIAGMN_22475 0.27 -2.3 4,438,958 + yeeZ NIAGMN_22475 0.27 -0.5 4,439,045 + yeeZ NIAGMN_22475 0.37 +0.6
Or see this region's nucleotide sequence