Strain Fitness in Escherichia coli ECRC102 around NIAGMN_22200

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyegD and alkA are separated by 181 nucleotidesalkA and mASE1 are separated by 100 nucleotides NIAGMN_22195: yegD - molecular chaperone, at 4,375,506 to 4,376,810 yegD NIAGMN_22200: alkA - DNA-3-methyladenine glycosylase 2, at 4,376,992 to 4,377,840 alkA NIAGMN_22205: mASE1 - diguanylate cyclase, at 4,377,941 to 4,380,748 mASE1 Position (kb) 4376 4377 4378Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 4376.245 kb on - strand, within yegDat 4376.245 kb on - strand, within yegDat 4376.257 kb on + strand, within yegDat 4376.412 kb on - strand, within yegDat 4376.573 kb on - strand, within yegDat 4376.573 kb on - strand, within yegDat 4376.573 kb on - strand, within yegDat 4376.640 kb on - strand, within yegDat 4376.641 kb on + strand, within yegDat 4376.839 kb on + strandat 4376.853 kb on - strandat 4376.863 kb on + strandat 4376.928 kb on + strandat 4376.961 kb on + strandat 4376.980 kb on + strandat 4376.995 kb on + strandat 4376.995 kb on + strandat 4376.995 kb on + strandat 4376.995 kb on + strandat 4376.997 kb on + strandat 4377.141 kb on + strand, within alkAat 4377.179 kb on + strand, within alkAat 4377.407 kb on + strand, within alkAat 4377.445 kb on + strand, within alkAat 4377.445 kb on + strand, within alkAat 4377.445 kb on + strand, within alkAat 4377.445 kb on + strand, within alkAat 4377.497 kb on + strand, within alkAat 4377.506 kb on + strand, within alkAat 4377.510 kb on + strand, within alkAat 4377.510 kb on + strand, within alkAat 4377.573 kb on - strand, within alkAat 4377.757 kb on + strandat 4377.801 kb on + strandat 4377.808 kb on + strandat 4377.810 kb on + strandat 4377.810 kb on + strandat 4377.810 kb on + strandat 4377.947 kb on - strandat 4377.958 kb on - strandat 4377.965 kb on - strandat 4378.144 kb on + strandat 4378.145 kb on - strandat 4378.145 kb on - strandat 4378.186 kb on - strandat 4378.195 kb on + strandat 4378.319 kb on - strand, within mASE1at 4378.328 kb on + strand, within mASE1at 4378.329 kb on - strand, within mASE1at 4378.550 kb on - strand, within mASE1

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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4,376,245 - yegD NIAGMN_22195 0.57 +2.9
4,376,245 - yegD NIAGMN_22195 0.57 +0.5
4,376,257 + yegD NIAGMN_22195 0.58 -1.7
4,376,412 - yegD NIAGMN_22195 0.69 -0.6
4,376,573 - yegD NIAGMN_22195 0.82 -2.5
4,376,573 - yegD NIAGMN_22195 0.82 -2.0
4,376,573 - yegD NIAGMN_22195 0.82 -0.3
4,376,640 - yegD NIAGMN_22195 0.87 +0.4
4,376,641 + yegD NIAGMN_22195 0.87 -0.2
4,376,839 + +2.6
4,376,853 - -2.5
4,376,863 + -1.2
4,376,928 + +0.5
4,376,961 + +0.2
4,376,980 + -2.2
4,376,995 + -2.6
4,376,995 + +0.8
4,376,995 + +2.2
4,376,995 + -2.3
4,376,997 + +0.4
4,377,141 + alkA NIAGMN_22200 0.18 +0.3
4,377,179 + alkA NIAGMN_22200 0.22 +3.1
4,377,407 + alkA NIAGMN_22200 0.49 +0.5
4,377,445 + alkA NIAGMN_22200 0.53 -2.9
4,377,445 + alkA NIAGMN_22200 0.53 +0.3
4,377,445 + alkA NIAGMN_22200 0.53 -1.3
4,377,445 + alkA NIAGMN_22200 0.53 -0.2
4,377,497 + alkA NIAGMN_22200 0.59 +0.1
4,377,506 + alkA NIAGMN_22200 0.61 -1.0
4,377,510 + alkA NIAGMN_22200 0.61 -0.6
4,377,510 + alkA NIAGMN_22200 0.61 +4.9
4,377,573 - alkA NIAGMN_22200 0.68 -0.8
4,377,757 + -0.2
4,377,801 + -1.7
4,377,808 + -1.1
4,377,810 + +0.7
4,377,810 + -0.5
4,377,810 + -1.3
4,377,947 - -0.7
4,377,958 - +0.1
4,377,965 - -0.8
4,378,144 + +0.0
4,378,145 - -2.5
4,378,145 - -1.3
4,378,186 - -1.3
4,378,195 + -1.5
4,378,319 - mASE1 NIAGMN_22205 0.13 -1.1
4,378,328 + mASE1 NIAGMN_22205 0.14 +0.3
4,378,329 - mASE1 NIAGMN_22205 0.14 -2.1
4,378,550 - mASE1 NIAGMN_22205 0.22 -0.9

Or see this region's nucleotide sequence