Strain Fitness in Escherichia coli ECRC102 around NIAGMN_06550

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsecF and secD are separated by 10 nucleotidessecD and yajC are separated by 60 nucleotidesyajC and tgt are separated by 22 nucleotides NIAGMN_06545: secF - Protein translocase subunit SecF, at 1,246,626 to 1,247,597 secF NIAGMN_06550: secD - Protein translocase subunit SecD, at 1,247,608 to 1,249,422 secD NIAGMN_06555: yajC - preprotein translocase subunit YajC, at 1,249,483 to 1,249,815 yajC NIAGMN_06560: tgt - tRNA guanosine(34) transglycosylase Tgt, at 1,249,838 to 1,250,965 tgt Position (kb) 1247 1248 1249 1250Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1248.742 kb on - strand, within secDat 1249.831 kb on - strandat 1249.831 kb on - strandat 1249.831 kb on - strandat 1249.834 kb on - strandat 1249.840 kb on - strandat 1249.840 kb on - strandat 1249.860 kb on - strandat 1249.905 kb on - strandat 1249.954 kb on - strand, within tgtat 1249.954 kb on - strand, within tgtat 1249.975 kb on + strand, within tgtat 1250.093 kb on - strand, within tgtat 1250.095 kb on - strand, within tgtat 1250.095 kb on - strand, within tgtat 1250.189 kb on - strand, within tgtat 1250.199 kb on - strand, within tgtat 1250.273 kb on + strand, within tgtat 1250.274 kb on - strand, within tgtat 1250.274 kb on - strand, within tgtat 1250.297 kb on - strand, within tgtat 1250.369 kb on - strand, within tgtat 1250.414 kb on - strand, within tgt

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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1,248,742 - secD NIAGMN_06550 0.62 -3.8
1,249,831 - +2.1
1,249,831 - +0.6
1,249,831 - +1.8
1,249,834 - +0.3
1,249,840 - -0.4
1,249,840 - -0.3
1,249,860 - -0.3
1,249,905 - +2.5
1,249,954 - tgt NIAGMN_06560 0.10 +0.7
1,249,954 - tgt NIAGMN_06560 0.10 -1.1
1,249,975 + tgt NIAGMN_06560 0.12 -2.4
1,250,093 - tgt NIAGMN_06560 0.23 -2.8
1,250,095 - tgt NIAGMN_06560 0.23 +0.5
1,250,095 - tgt NIAGMN_06560 0.23 -2.6
1,250,189 - tgt NIAGMN_06560 0.31 +1.6
1,250,199 - tgt NIAGMN_06560 0.32 +2.0
1,250,273 + tgt NIAGMN_06560 0.39 -0.7
1,250,274 - tgt NIAGMN_06560 0.39 +2.2
1,250,274 - tgt NIAGMN_06560 0.39 +0.9
1,250,297 - tgt NIAGMN_06560 0.41 +0.0
1,250,369 - tgt NIAGMN_06560 0.47 -0.4
1,250,414 - tgt NIAGMN_06560 0.51 -0.2

Or see this region's nucleotide sequence