Strain Fitness in Escherichia coli ECRC102 around NIAGMN_03010

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycbX and zapC overlap by 4 nucleotideszapC and pyrD are separated by 173 nucleotides NIAGMN_03005: ycbX - 6-N-hydroxylaminopurine resistance protein YcbX, at 517,721 to 518,830 ycbX NIAGMN_03010: zapC - cell division protein ZapC, at 518,827 to 519,369 zapC NIAGMN_03015: pyrD - quinone-dependent dihydroorotate dehydrogenase, at 519,543 to 520,553 pyrD Position (kb) 518 519 520Strain fitness (log2 ratio) -2 -1 0 1 2 3at 517.950 kb on - strand, within ycbXat 518.079 kb on + strand, within ycbXat 518.184 kb on + strand, within ycbXat 518.278 kb on + strand, within ycbXat 518.323 kb on + strand, within ycbXat 518.473 kb on + strand, within ycbXat 518.895 kb on + strand, within zapCat 518.959 kb on + strand, within zapCat 519.200 kb on + strand, within zapCat 519.289 kb on - strand, within zapCat 519.429 kb on - strandat 519.441 kb on - strandat 519.461 kb on + strandat 519.514 kb on - strandat 519.519 kb on - strandat 519.816 kb on - strand, within pyrDat 519.853 kb on - strand, within pyrDat 520.023 kb on - strand, within pyrDat 520.183 kb on - strand, within pyrD

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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517,950 - ycbX NIAGMN_03005 0.21 +0.3
518,079 + ycbX NIAGMN_03005 0.32 -2.2
518,184 + ycbX NIAGMN_03005 0.42 -1.8
518,278 + ycbX NIAGMN_03005 0.50 -2.1
518,323 + ycbX NIAGMN_03005 0.54 -0.6
518,473 + ycbX NIAGMN_03005 0.68 +0.0
518,895 + zapC NIAGMN_03010 0.13 -2.2
518,959 + zapC NIAGMN_03010 0.24 -0.4
519,200 + zapC NIAGMN_03010 0.69 -0.6
519,289 - zapC NIAGMN_03010 0.85 -0.2
519,429 - -1.4
519,441 - +1.0
519,461 + +1.3
519,514 - +2.8
519,519 - +0.0
519,816 - pyrD NIAGMN_03015 0.27 -1.2
519,853 - pyrD NIAGMN_03015 0.31 -1.3
520,023 - pyrD NIAGMN_03015 0.47 -2.2
520,183 - pyrD NIAGMN_03015 0.63 +1.0

Or see this region's nucleotide sequence