Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00965

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntbtuD and btuE overlap by 1 nucleotidesbtuE and btuC are separated by 62 nucleotidesbtuC and NIAGMN_00975 are separated by 100 nucleotidesNIAGMN_00975 and pheT are separated by 4 nucleotides NIAGMN_00955: btuD - vitamin B12 ABC transporter ATP-binding protein BtuD, at 168,094 to 168,843 btuD NIAGMN_00960: btuE - bifunctional thioredoxin/glutathione peroxidase, at 168,843 to 169,394 btuE NIAGMN_00965: btuC - vitamin B12 ABC transporter permease BtuC, at 169,457 to 170,437 btuC NIAGMN_00975: NIAGMN_00975 - Integration host factor subunit alpha, at 170,538 to 170,837 _00975 NIAGMN_00980: pheT - phenylalanine--tRNA ligase subunit beta, at 170,842 to 173,229 pheT Position (kb) 169 170 171Strain fitness (log2 ratio) -2 -1 0 1 2at 168.527 kb on - strand, within btuDat 168.730 kb on - strand, within btuDat 169.526 kb on - strandat 169.651 kb on + strand, within btuCat 169.673 kb on - strand, within btuCat 169.761 kb on - strand, within btuCat 169.975 kb on + strand, within btuCat 170.441 kb on - strandat 170.526 kb on - strandat 170.530 kb on - strandat 170.670 kb on - strand, within NIAGMN_00975at 170.670 kb on - strand, within NIAGMN_00975at 170.688 kb on - strand, within NIAGMN_00975at 170.784 kb on - strand, within NIAGMN_00975

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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168,527 - btuD NIAGMN_00955 0.58 +0.5
168,730 - btuD NIAGMN_00955 0.85 +0.3
169,526 - +0.6
169,651 + btuC NIAGMN_00965 0.20 +0.9
169,673 - btuC NIAGMN_00965 0.22 -0.1
169,761 - btuC NIAGMN_00965 0.31 -0.1
169,975 + btuC NIAGMN_00965 0.53 -0.9
170,441 - +0.5
170,526 - +0.1
170,530 - -0.7
170,670 - NIAGMN_00975 0.44 +2.7
170,670 - NIAGMN_00975 0.44 +1.4
170,688 - NIAGMN_00975 0.50 +0.1
170,784 - NIAGMN_00975 0.82 -2.1

Or see this region's nucleotide sequence