Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt btuD and btuE overlap by 1 nucleotides btuE and btuC are separated by 62 nucleotides btuC and MCAODC_18320 are separated by 100 nucleotides MCAODC_18320 and pheT are separated by 4 nucleotides
MCAODC_18300: btuD - vitamin B12 ABC transporter ATP-binding protein BtuD, at 521,791 to 522,540
btuD
MCAODC_18305: btuE - bifunctional thioredoxin/glutathione peroxidase, at 522,540 to 523,091
btuE
MCAODC_18310: btuC - vitamin B12 ABC transporter permease BtuC, at 523,154 to 524,134
btuC
MCAODC_18320: MCAODC_18320 - Integration host factor subunit alpha, at 524,235 to 524,534
_18320
MCAODC_18325: pheT - phenylalanine--tRNA ligase subunit beta, at 524,539 to 526,926
pheT
Position (kb) 523
524
525 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 522.224 kb on - strand, within btuD at 522.520 kb on + strand at 522.521 kb on - strand at 522.561 kb on - strand at 522.597 kb on - strand, within btuE at 523.124 kb on - strand at 523.242 kb on - strand at 523.349 kb on - strand, within btuC at 523.494 kb on - strand, within btuC at 523.494 kb on - strand, within btuC at 523.499 kb on - strand, within btuC at 523.499 kb on - strand, within btuC at 523.499 kb on - strand, within btuC at 523.578 kb on - strand, within btuC at 523.606 kb on - strand, within btuC at 523.620 kb on - strand, within btuC at 523.645 kb on + strand, within btuC at 523.743 kb on - strand, within btuC at 523.756 kb on + strand, within btuC at 523.866 kb on - strand, within btuC at 524.048 kb on + strand at 524.227 kb on - strand at 524.526 kb on - strand at 524.535 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 522,224 - btuD MCAODC_18300 0.58 -1.3 522,520 + +1.3 522,521 - -2.2 522,561 - -2.3 522,597 - btuE MCAODC_18305 0.10 -1.1 523,124 - -2.4 523,242 - -1.9 523,349 - btuC MCAODC_18310 0.20 +3.1 523,494 - btuC MCAODC_18310 0.35 -0.1 523,494 - btuC MCAODC_18310 0.35 -1.6 523,499 - btuC MCAODC_18310 0.35 -1.4 523,499 - btuC MCAODC_18310 0.35 -2.8 523,499 - btuC MCAODC_18310 0.35 +1.4 523,578 - btuC MCAODC_18310 0.43 +0.9 523,606 - btuC MCAODC_18310 0.46 -0.5 523,620 - btuC MCAODC_18310 0.48 +0.1 523,645 + btuC MCAODC_18310 0.50 +0.3 523,743 - btuC MCAODC_18310 0.60 +0.9 523,756 + btuC MCAODC_18310 0.61 +1.0 523,866 - btuC MCAODC_18310 0.73 -1.1 524,048 + -1.8 524,227 - -0.8 524,526 - -0.9 524,535 - -3.8
Or see this region's nucleotide sequence