Experiment: Cytosine 0.9 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS12075 and A4249_RS12080 are separated by 97 nucleotides A4249_RS12080 and A4249_RS12085 overlap by 1 nucleotides A4249_RS12085 and A4249_RS12090 are separated by 137 nucleotides A4249_RS12090 and A4249_RS12095 overlap by 4 nucleotides A4249_RS12095 and A4249_RS12100 are separated by 95 nucleotides
A4249_RS12075: A4249_RS12075 - DUF1328 domain-containing protein, at 2,422,533 to 2,422,712
_RS12075
A4249_RS12080: A4249_RS12080 - hypothetical protein, at 2,422,810 to 2,423,220
_RS12080
A4249_RS12085: A4249_RS12085 - DUF883 family protein, at 2,423,220 to 2,423,564
_RS12085
A4249_RS12090: A4249_RS12090 - fumarylacetoacetate hydrolase family protein, at 2,423,702 to 2,424,370
_RS12090
A4249_RS12095: A4249_RS12095 - maleylacetoacetate isomerase, at 2,424,367 to 2,425,002
_RS12095
A4249_RS12100: A4249_RS12100 - aldo/keto reductase, at 2,425,098 to 2,426,018
_RS12100
Position (kb)
2423
2424
2425 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2422.718 kb on + strand at 2422.718 kb on + strand at 2422.719 kb on - strand at 2423.320 kb on + strand, within A4249_RS12085 at 2423.320 kb on + strand, within A4249_RS12085 at 2423.320 kb on + strand, within A4249_RS12085 at 2423.321 kb on - strand, within A4249_RS12085 at 2423.321 kb on - strand, within A4249_RS12085 at 2423.321 kb on - strand, within A4249_RS12085 at 2423.321 kb on - strand, within A4249_RS12085 at 2423.321 kb on - strand, within A4249_RS12085 at 2423.321 kb on - strand, within A4249_RS12085 at 2423.321 kb on - strand, within A4249_RS12085 at 2423.393 kb on - strand, within A4249_RS12085 at 2423.393 kb on - strand, within A4249_RS12085 at 2423.396 kb on - strand, within A4249_RS12085 at 2423.538 kb on - strand at 2423.594 kb on - strand at 2423.599 kb on - strand at 2423.643 kb on + strand at 2423.643 kb on + strand at 2423.644 kb on - strand at 2423.644 kb on - strand at 2423.644 kb on - strand at 2423.644 kb on - strand at 2423.677 kb on + strand at 2423.678 kb on - strand at 2423.689 kb on + strand at 2423.690 kb on - strand at 2423.690 kb on - strand at 2423.690 kb on - strand at 2423.690 kb on - strand at 2423.690 kb on - strand at 2423.804 kb on + strand, within A4249_RS12090 at 2423.804 kb on + strand, within A4249_RS12090 at 2423.804 kb on + strand, within A4249_RS12090 at 2423.805 kb on - strand, within A4249_RS12090 at 2423.805 kb on - strand, within A4249_RS12090 at 2423.805 kb on - strand, within A4249_RS12090 at 2423.805 kb on - strand, within A4249_RS12090 at 2423.805 kb on - strand, within A4249_RS12090 at 2423.909 kb on + strand, within A4249_RS12090 at 2423.936 kb on + strand, within A4249_RS12090 at 2423.936 kb on + strand, within A4249_RS12090 at 2423.936 kb on + strand, within A4249_RS12090 at 2423.937 kb on - strand, within A4249_RS12090 at 2423.937 kb on - strand, within A4249_RS12090 at 2423.937 kb on - strand, within A4249_RS12090 at 2425.022 kb on - strand at 2425.034 kb on + strand at 2425.034 kb on + strand at 2425.034 kb on + strand at 2425.034 kb on + strand at 2425.034 kb on + strand at 2425.034 kb on + strand at 2425.035 kb on - strand at 2425.035 kb on - strand at 2425.035 kb on - strand at 2425.035 kb on - strand at 2425.038 kb on + strand at 2425.039 kb on - strand at 2425.039 kb on - strand at 2425.039 kb on - strand at 2425.039 kb on - strand at 2425.049 kb on + strand at 2425.088 kb on - strand at 2425.088 kb on - strand at 2425.088 kb on - strand at 2425.088 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine 0.9 mM (C) remove 2,422,718 + -1.3 2,422,718 + +0.4 2,422,719 - +0.0 2,423,320 + A4249_RS12085 0.29 +2.2 2,423,320 + A4249_RS12085 0.29 +0.0 2,423,320 + A4249_RS12085 0.29 +0.6 2,423,321 - A4249_RS12085 0.29 +0.0 2,423,321 - A4249_RS12085 0.29 -1.3 2,423,321 - A4249_RS12085 0.29 -0.7 2,423,321 - A4249_RS12085 0.29 +0.0 2,423,321 - A4249_RS12085 0.29 +0.4 2,423,321 - A4249_RS12085 0.29 -0.8 2,423,321 - A4249_RS12085 0.29 +0.6 2,423,393 - A4249_RS12085 0.50 +1.2 2,423,393 - A4249_RS12085 0.50 -2.2 2,423,396 - A4249_RS12085 0.51 -0.1 2,423,538 - +1.6 2,423,594 - -0.2 2,423,599 - -0.4 2,423,643 + +1.0 2,423,643 + -0.6 2,423,644 - -0.1 2,423,644 - +0.0 2,423,644 - -0.2 2,423,644 - -0.1 2,423,677 + -1.3 2,423,678 - -0.1 2,423,689 + -0.6 2,423,690 - -0.6 2,423,690 - -0.2 2,423,690 - +0.2 2,423,690 - -1.0 2,423,690 - -0.2 2,423,804 + A4249_RS12090 0.15 -1.2 2,423,804 + A4249_RS12090 0.15 -0.5 2,423,804 + A4249_RS12090 0.15 -0.8 2,423,805 - A4249_RS12090 0.15 +0.1 2,423,805 - A4249_RS12090 0.15 +0.5 2,423,805 - A4249_RS12090 0.15 -0.4 2,423,805 - A4249_RS12090 0.15 -0.1 2,423,805 - A4249_RS12090 0.15 -0.4 2,423,909 + A4249_RS12090 0.31 -0.3 2,423,936 + A4249_RS12090 0.35 -0.9 2,423,936 + A4249_RS12090 0.35 -1.4 2,423,936 + A4249_RS12090 0.35 -0.5 2,423,937 - A4249_RS12090 0.35 +0.5 2,423,937 - A4249_RS12090 0.35 -0.1 2,423,937 - A4249_RS12090 0.35 -0.2 2,425,022 - -2.1 2,425,034 + +0.4 2,425,034 + +0.7 2,425,034 + +0.2 2,425,034 + -0.5 2,425,034 + -0.7 2,425,034 + +0.9 2,425,035 - +1.6 2,425,035 - -0.7 2,425,035 - -1.7 2,425,035 - -1.6 2,425,038 + -0.5 2,425,039 - -1.3 2,425,039 - +0.6 2,425,039 - -0.0 2,425,039 - +0.1 2,425,049 + +0.7 2,425,088 - -1.1 2,425,088 - -1.1 2,425,088 - +1.0 2,425,088 - -1.0
Or see this region's nucleotide sequence