Experiment: Cytosine 0.9 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS00995 and A4249_RS01000 are separated by 40 nucleotides A4249_RS01000 and A4249_RS01005 are separated by 4 nucleotides A4249_RS01005 and A4249_RS01010 are separated by 114 nucleotides
A4249_RS00995: A4249_RS00995 - M48 family metallopeptidase, at 187,622 to 188,827
_RS00995
A4249_RS01000: A4249_RS01000 - DoxX family protein, at 188,868 to 189,329
_RS01000
A4249_RS01005: A4249_RS01005 - beta-ketoacyl-ACP synthase III, at 189,334 to 190,488
_RS01005
A4249_RS01010: A4249_RS01010 - capsular polysaccharide biosynthesis protein, at 190,603 to 192,657
_RS01010
Position (kb) 189
190
191 Strain fitness (log2 ratio) -2
-1
0
1
2 at 188.384 kb on + strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.385 kb on - strand, within A4249_RS00995 at 188.466 kb on - strand, within A4249_RS00995 at 188.588 kb on - strand, within A4249_RS00995 at 188.687 kb on + strand, within A4249_RS00995 at 188.729 kb on + strand at 188.729 kb on + strand at 188.729 kb on + strand at 188.730 kb on - strand at 188.881 kb on - strand at 188.976 kb on + strand, within A4249_RS01000 at 188.976 kb on + strand, within A4249_RS01000 at 188.976 kb on + strand, within A4249_RS01000 at 188.976 kb on + strand, within A4249_RS01000 at 188.977 kb on - strand, within A4249_RS01000 at 188.977 kb on - strand, within A4249_RS01000 at 188.977 kb on - strand, within A4249_RS01000 at 188.977 kb on - strand, within A4249_RS01000 at 189.222 kb on + strand, within A4249_RS01000 at 189.222 kb on + strand, within A4249_RS01000 at 189.222 kb on + strand, within A4249_RS01000 at 189.327 kb on + strand at 189.476 kb on + strand, within A4249_RS01005 at 189.477 kb on - strand, within A4249_RS01005 at 189.477 kb on - strand, within A4249_RS01005 at 189.556 kb on + strand, within A4249_RS01005 at 189.586 kb on + strand, within A4249_RS01005 at 189.586 kb on + strand, within A4249_RS01005 at 189.586 kb on + strand, within A4249_RS01005 at 189.586 kb on + strand, within A4249_RS01005 at 189.587 kb on - strand, within A4249_RS01005 at 189.587 kb on - strand, within A4249_RS01005 at 189.587 kb on - strand, within A4249_RS01005 at 189.587 kb on - strand, within A4249_RS01005 at 189.587 kb on - strand, within A4249_RS01005 at 189.587 kb on - strand, within A4249_RS01005 at 189.587 kb on - strand, within A4249_RS01005 at 189.587 kb on - strand, within A4249_RS01005 at 189.783 kb on + strand, within A4249_RS01005 at 189.783 kb on + strand, within A4249_RS01005 at 189.784 kb on - strand, within A4249_RS01005 at 189.784 kb on - strand, within A4249_RS01005 at 189.956 kb on - strand, within A4249_RS01005 at 189.979 kb on + strand, within A4249_RS01005 at 190.027 kb on + strand, within A4249_RS01005 at 190.027 kb on + strand, within A4249_RS01005 at 190.027 kb on + strand, within A4249_RS01005 at 190.027 kb on + strand, within A4249_RS01005 at 190.027 kb on + strand, within A4249_RS01005 at 190.027 kb on + strand, within A4249_RS01005 at 190.028 kb on - strand, within A4249_RS01005 at 190.028 kb on - strand, within A4249_RS01005 at 190.028 kb on - strand, within A4249_RS01005 at 190.028 kb on - strand, within A4249_RS01005 at 190.028 kb on - strand, within A4249_RS01005 at 190.028 kb on - strand, within A4249_RS01005 at 190.094 kb on + strand, within A4249_RS01005 at 190.409 kb on + strand at 190.409 kb on + strand at 190.409 kb on + strand at 190.409 kb on + strand at 190.409 kb on + strand at 190.409 kb on + strand at 190.409 kb on + strand at 190.409 kb on + strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.410 kb on - strand at 190.500 kb on + strand at 190.500 kb on + strand at 190.545 kb on - strand at 190.607 kb on + strand at 190.608 kb on - strand at 190.608 kb on - strand at 190.608 kb on - strand at 190.763 kb on + strand at 190.763 kb on + strand at 190.763 kb on + strand at 190.764 kb on - strand at 190.764 kb on - strand at 190.764 kb on - strand at 190.764 kb on - strand at 190.764 kb on - strand at 190.772 kb on + strand at 190.868 kb on + strand, within A4249_RS01010 at 190.868 kb on + strand, within A4249_RS01010 at 190.869 kb on - strand, within A4249_RS01010 at 190.869 kb on - strand, within A4249_RS01010 at 190.883 kb on + strand, within A4249_RS01010 at 190.884 kb on - strand, within A4249_RS01010 at 190.884 kb on - strand, within A4249_RS01010 at 191.073 kb on - strand, within A4249_RS01010 at 191.079 kb on + strand, within A4249_RS01010 at 191.079 kb on + strand, within A4249_RS01010 at 191.080 kb on - strand, within A4249_RS01010 at 191.080 kb on - strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.244 kb on + strand, within A4249_RS01010 at 191.245 kb on - strand, within A4249_RS01010 at 191.246 kb on + strand, within A4249_RS01010 at 191.246 kb on + strand, within A4249_RS01010 at 191.246 kb on + strand, within A4249_RS01010 at 191.246 kb on + strand, within A4249_RS01010 at 191.247 kb on - strand, within A4249_RS01010 at 191.247 kb on - strand, within A4249_RS01010 at 191.247 kb on - strand, within A4249_RS01010 at 191.366 kb on + strand, within A4249_RS01010 at 191.366 kb on + strand, within A4249_RS01010 at 191.366 kb on + strand, within A4249_RS01010 at 191.367 kb on - strand, within A4249_RS01010 at 191.367 kb on - strand, within A4249_RS01010 at 191.369 kb on + strand, within A4249_RS01010 at 191.369 kb on + strand, within A4249_RS01010 at 191.369 kb on + strand, within A4249_RS01010 at 191.369 kb on + strand, within A4249_RS01010 at 191.369 kb on + strand, within A4249_RS01010 at 191.369 kb on + strand, within A4249_RS01010 at 191.370 kb on - strand, within A4249_RS01010 at 191.370 kb on - strand, within A4249_RS01010 at 191.370 kb on - strand, within A4249_RS01010 at 191.370 kb on - strand, within A4249_RS01010
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine 0.9 mM (C) remove 188,384 + A4249_RS00995 0.63 -0.0 188,385 - A4249_RS00995 0.63 +0.7 188,385 - A4249_RS00995 0.63 +0.0 188,385 - A4249_RS00995 0.63 -0.8 188,385 - A4249_RS00995 0.63 -0.1 188,385 - A4249_RS00995 0.63 -0.1 188,385 - A4249_RS00995 0.63 +0.3 188,385 - A4249_RS00995 0.63 +0.8 188,385 - A4249_RS00995 0.63 -0.0 188,466 - A4249_RS00995 0.70 -0.6 188,588 - A4249_RS00995 0.80 -0.8 188,687 + A4249_RS00995 0.88 +0.2 188,729 + +1.2 188,729 + -0.4 188,729 + +0.0 188,730 - -0.1 188,881 - +0.0 188,976 + A4249_RS01000 0.23 -1.1 188,976 + A4249_RS01000 0.23 +0.0 188,976 + A4249_RS01000 0.23 -1.0 188,976 + A4249_RS01000 0.23 -0.1 188,977 - A4249_RS01000 0.24 -0.4 188,977 - A4249_RS01000 0.24 +0.0 188,977 - A4249_RS01000 0.24 +0.5 188,977 - A4249_RS01000 0.24 -0.2 189,222 + A4249_RS01000 0.77 -0.1 189,222 + A4249_RS01000 0.77 +0.2 189,222 + A4249_RS01000 0.77 +0.1 189,327 + -0.1 189,476 + A4249_RS01005 0.12 -0.1 189,477 - A4249_RS01005 0.12 -0.2 189,477 - A4249_RS01005 0.12 -0.0 189,556 + A4249_RS01005 0.19 -1.5 189,586 + A4249_RS01005 0.22 -0.2 189,586 + A4249_RS01005 0.22 -0.2 189,586 + A4249_RS01005 0.22 -0.2 189,586 + A4249_RS01005 0.22 -0.3 189,587 - A4249_RS01005 0.22 +0.3 189,587 - A4249_RS01005 0.22 +1.2 189,587 - A4249_RS01005 0.22 +0.0 189,587 - A4249_RS01005 0.22 +0.6 189,587 - A4249_RS01005 0.22 -0.8 189,587 - A4249_RS01005 0.22 -0.3 189,587 - A4249_RS01005 0.22 +1.0 189,587 - A4249_RS01005 0.22 +0.4 189,783 + A4249_RS01005 0.39 +1.5 189,783 + A4249_RS01005 0.39 +0.0 189,784 - A4249_RS01005 0.39 -0.7 189,784 - A4249_RS01005 0.39 +0.2 189,956 - A4249_RS01005 0.54 -0.1 189,979 + A4249_RS01005 0.56 -0.5 190,027 + A4249_RS01005 0.60 +1.5 190,027 + A4249_RS01005 0.60 +0.0 190,027 + A4249_RS01005 0.60 +0.6 190,027 + A4249_RS01005 0.60 +0.4 190,027 + A4249_RS01005 0.60 +0.4 190,027 + A4249_RS01005 0.60 +0.2 190,028 - A4249_RS01005 0.60 -0.2 190,028 - A4249_RS01005 0.60 -0.8 190,028 - A4249_RS01005 0.60 -0.5 190,028 - A4249_RS01005 0.60 -0.2 190,028 - A4249_RS01005 0.60 +0.9 190,028 - A4249_RS01005 0.60 -0.1 190,094 + A4249_RS01005 0.66 -1.7 190,409 + +0.6 190,409 + -0.3 190,409 + -1.6 190,409 + -0.1 190,409 + +0.2 190,409 + +0.9 190,409 + +0.5 190,409 + +0.4 190,410 - -1.7 190,410 - -0.5 190,410 - +1.3 190,410 - -0.3 190,410 - +0.3 190,410 - -0.1 190,410 - -0.4 190,410 - -0.4 190,410 - +1.2 190,410 - -0.1 190,410 - +1.7 190,500 + -0.2 190,500 + -0.4 190,545 - -0.1 190,607 + +1.0 190,608 - -0.3 190,608 - +0.3 190,608 - +0.0 190,763 + -0.2 190,763 + +0.2 190,763 + -1.5 190,764 - +1.3 190,764 - -2.5 190,764 - +0.7 190,764 - -1.4 190,764 - +1.2 190,772 + -0.2 190,868 + A4249_RS01010 0.13 -0.2 190,868 + A4249_RS01010 0.13 -0.2 190,869 - A4249_RS01010 0.13 -1.2 190,869 - A4249_RS01010 0.13 -0.8 190,883 + A4249_RS01010 0.14 +1.2 190,884 - A4249_RS01010 0.14 -0.4 190,884 - A4249_RS01010 0.14 -0.5 191,073 - A4249_RS01010 0.23 +2.2 191,079 + A4249_RS01010 0.23 -1.2 191,079 + A4249_RS01010 0.23 -1.4 191,080 - A4249_RS01010 0.23 -0.7 191,080 - A4249_RS01010 0.23 -0.4 191,244 + A4249_RS01010 0.31 +0.3 191,244 + A4249_RS01010 0.31 +0.3 191,244 + A4249_RS01010 0.31 -1.2 191,244 + A4249_RS01010 0.31 -0.2 191,244 + A4249_RS01010 0.31 +0.4 191,244 + A4249_RS01010 0.31 -1.2 191,244 + A4249_RS01010 0.31 -1.0 191,244 + A4249_RS01010 0.31 +0.0 191,245 - A4249_RS01010 0.31 +0.4 191,246 + A4249_RS01010 0.31 -0.7 191,246 + A4249_RS01010 0.31 -0.2 191,246 + A4249_RS01010 0.31 -0.2 191,246 + A4249_RS01010 0.31 -1.5 191,247 - A4249_RS01010 0.31 -0.6 191,247 - A4249_RS01010 0.31 -0.5 191,247 - A4249_RS01010 0.31 +0.0 191,366 + A4249_RS01010 0.37 -1.4 191,366 + A4249_RS01010 0.37 -1.2 191,366 + A4249_RS01010 0.37 -0.2 191,367 - A4249_RS01010 0.37 +0.0 191,367 - A4249_RS01010 0.37 +0.0 191,369 + A4249_RS01010 0.37 -2.2 191,369 + A4249_RS01010 0.37 -1.8 191,369 + A4249_RS01010 0.37 -0.9 191,369 + A4249_RS01010 0.37 +0.0 191,369 + A4249_RS01010 0.37 -2.5 191,369 + A4249_RS01010 0.37 -1.5 191,370 - A4249_RS01010 0.37 +1.2 191,370 - A4249_RS01010 0.37 +1.0 191,370 - A4249_RS01010 0.37 -0.4 191,370 - A4249_RS01010 0.37 +1.9
Or see this region's nucleotide sequence