Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS11545 and A4249_RS11550 are separated by 3 nucleotides A4249_RS11550 and A4249_RS11555 are separated by 25 nucleotides A4249_RS11555 and A4249_RS11560 overlap by 4 nucleotides A4249_RS11560 and A4249_RS11565 are separated by 104 nucleotides
A4249_RS11545: A4249_RS11545 - L,D-transpeptidase family protein, at 2,323,702 to 2,325,192
_RS11545
A4249_RS11550: A4249_RS11550 - cupin domain-containing protein, at 2,325,196 to 2,325,591
_RS11550
A4249_RS11555: A4249_RS11555 - metalloregulator ArsR/SmtB family transcription factor, at 2,325,617 to 2,326,036
_RS11555
A4249_RS11560: A4249_RS11560 - peroxiredoxin, at 2,326,033 to 2,326,692
_RS11560
A4249_RS11565: A4249_RS11565 - MBL fold metallo-hydrolase, at 2,326,797 to 2,327,738
_RS11565
Position (kb)
2325
2326
2327 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2324.722 kb on + strand, within A4249_RS11545 at 2324.722 kb on + strand, within A4249_RS11545 at 2324.722 kb on + strand, within A4249_RS11545 at 2324.722 kb on + strand, within A4249_RS11545 at 2324.722 kb on + strand, within A4249_RS11545 at 2324.723 kb on - strand, within A4249_RS11545 at 2324.723 kb on - strand, within A4249_RS11545 at 2324.723 kb on - strand, within A4249_RS11545 at 2324.723 kb on - strand, within A4249_RS11545 at 2324.723 kb on - strand, within A4249_RS11545 at 2324.723 kb on - strand, within A4249_RS11545 at 2324.724 kb on + strand, within A4249_RS11545 at 2324.724 kb on + strand, within A4249_RS11545 at 2324.724 kb on + strand, within A4249_RS11545 at 2324.724 kb on + strand, within A4249_RS11545 at 2324.725 kb on - strand, within A4249_RS11545 at 2324.725 kb on - strand, within A4249_RS11545 at 2324.725 kb on - strand, within A4249_RS11545 at 2324.725 kb on - strand, within A4249_RS11545 at 2324.824 kb on + strand, within A4249_RS11545 at 2324.824 kb on + strand, within A4249_RS11545 at 2324.824 kb on + strand, within A4249_RS11545 at 2324.824 kb on + strand, within A4249_RS11545 at 2324.824 kb on + strand, within A4249_RS11545 at 2324.824 kb on + strand, within A4249_RS11545 at 2324.824 kb on + strand, within A4249_RS11545 at 2324.825 kb on - strand, within A4249_RS11545 at 2324.825 kb on - strand, within A4249_RS11545 at 2324.825 kb on - strand, within A4249_RS11545 at 2324.825 kb on - strand, within A4249_RS11545 at 2324.825 kb on - strand, within A4249_RS11545 at 2325.040 kb on + strand, within A4249_RS11545 at 2325.041 kb on - strand, within A4249_RS11545 at 2325.041 kb on - strand, within A4249_RS11545 at 2325.041 kb on - strand, within A4249_RS11545 at 2325.041 kb on - strand, within A4249_RS11545 at 2325.094 kb on + strand at 2325.094 kb on + strand at 2325.094 kb on + strand at 2325.095 kb on - strand at 2325.095 kb on - strand at 2325.095 kb on - strand at 2325.095 kb on - strand at 2325.264 kb on + strand, within A4249_RS11550 at 2325.290 kb on + strand, within A4249_RS11550 at 2325.290 kb on + strand, within A4249_RS11550 at 2325.290 kb on + strand, within A4249_RS11550 at 2325.290 kb on + strand, within A4249_RS11550 at 2325.290 kb on + strand, within A4249_RS11550 at 2325.291 kb on - strand, within A4249_RS11550 at 2325.291 kb on - strand, within A4249_RS11550 at 2325.291 kb on - strand, within A4249_RS11550 at 2325.291 kb on - strand, within A4249_RS11550 at 2325.300 kb on - strand, within A4249_RS11550 at 2325.300 kb on - strand, within A4249_RS11550 at 2325.300 kb on - strand, within A4249_RS11550 at 2325.524 kb on + strand, within A4249_RS11550 at 2325.524 kb on + strand, within A4249_RS11550 at 2325.525 kb on - strand, within A4249_RS11550 at 2325.571 kb on - strand at 2325.571 kb on - strand at 2325.744 kb on + strand, within A4249_RS11555 at 2325.790 kb on - strand, within A4249_RS11555 at 2326.052 kb on + strand at 2326.053 kb on - strand at 2326.053 kb on - strand at 2326.056 kb on - strand at 2326.056 kb on - strand at 2326.056 kb on - strand at 2326.056 kb on - strand at 2326.398 kb on - strand, within A4249_RS11560 at 2326.398 kb on - strand, within A4249_RS11560 at 2326.398 kb on - strand, within A4249_RS11560 at 2326.427 kb on + strand, within A4249_RS11560 at 2326.572 kb on - strand, within A4249_RS11560 at 2326.704 kb on - strand at 2326.704 kb on - strand at 2326.704 kb on - strand at 2326.727 kb on - strand at 2326.766 kb on - strand at 2326.980 kb on + strand, within A4249_RS11565 at 2326.980 kb on + strand, within A4249_RS11565 at 2326.980 kb on + strand, within A4249_RS11565 at 2326.980 kb on + strand, within A4249_RS11565 at 2326.981 kb on - strand, within A4249_RS11565 at 2327.034 kb on + strand, within A4249_RS11565 at 2327.035 kb on - strand, within A4249_RS11565
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 2,324,722 + A4249_RS11545 0.68 +0.6 2,324,722 + A4249_RS11545 0.68 +0.5 2,324,722 + A4249_RS11545 0.68 +0.9 2,324,722 + A4249_RS11545 0.68 -1.4 2,324,722 + A4249_RS11545 0.68 +1.2 2,324,723 - A4249_RS11545 0.68 +1.2 2,324,723 - A4249_RS11545 0.68 -0.7 2,324,723 - A4249_RS11545 0.68 +0.1 2,324,723 - A4249_RS11545 0.68 +0.0 2,324,723 - A4249_RS11545 0.68 -0.5 2,324,723 - A4249_RS11545 0.68 +0.7 2,324,724 + A4249_RS11545 0.69 -0.2 2,324,724 + A4249_RS11545 0.69 -0.4 2,324,724 + A4249_RS11545 0.69 -0.5 2,324,724 + A4249_RS11545 0.69 -0.0 2,324,725 - A4249_RS11545 0.69 -0.4 2,324,725 - A4249_RS11545 0.69 -0.2 2,324,725 - A4249_RS11545 0.69 +0.3 2,324,725 - A4249_RS11545 0.69 +0.9 2,324,824 + A4249_RS11545 0.75 -0.3 2,324,824 + A4249_RS11545 0.75 +1.2 2,324,824 + A4249_RS11545 0.75 -0.7 2,324,824 + A4249_RS11545 0.75 -0.6 2,324,824 + A4249_RS11545 0.75 +0.2 2,324,824 + A4249_RS11545 0.75 -0.2 2,324,824 + A4249_RS11545 0.75 +0.4 2,324,825 - A4249_RS11545 0.75 +0.6 2,324,825 - A4249_RS11545 0.75 +0.0 2,324,825 - A4249_RS11545 0.75 +0.9 2,324,825 - A4249_RS11545 0.75 -1.2 2,324,825 - A4249_RS11545 0.75 +0.0 2,325,040 + A4249_RS11545 0.90 +1.3 2,325,041 - A4249_RS11545 0.90 -1.8 2,325,041 - A4249_RS11545 0.90 +0.6 2,325,041 - A4249_RS11545 0.90 +0.7 2,325,041 - A4249_RS11545 0.90 -1.3 2,325,094 + -0.3 2,325,094 + +1.2 2,325,094 + +0.0 2,325,095 - +1.7 2,325,095 - -2.4 2,325,095 - +0.1 2,325,095 - +0.5 2,325,264 + A4249_RS11550 0.17 -0.5 2,325,290 + A4249_RS11550 0.24 +0.3 2,325,290 + A4249_RS11550 0.24 +1.5 2,325,290 + A4249_RS11550 0.24 -0.7 2,325,290 + A4249_RS11550 0.24 +0.2 2,325,290 + A4249_RS11550 0.24 -0.7 2,325,291 - A4249_RS11550 0.24 -0.2 2,325,291 - A4249_RS11550 0.24 +0.6 2,325,291 - A4249_RS11550 0.24 +0.2 2,325,291 - A4249_RS11550 0.24 -0.8 2,325,300 - A4249_RS11550 0.26 -0.1 2,325,300 - A4249_RS11550 0.26 -0.8 2,325,300 - A4249_RS11550 0.26 -0.8 2,325,524 + A4249_RS11550 0.83 -0.3 2,325,524 + A4249_RS11550 0.83 -1.2 2,325,525 - A4249_RS11550 0.83 +0.2 2,325,571 - -0.3 2,325,571 - -0.3 2,325,744 + A4249_RS11555 0.30 +1.2 2,325,790 - A4249_RS11555 0.41 -1.8 2,326,052 + +0.8 2,326,053 - -0.2 2,326,053 - -0.4 2,326,056 - -1.2 2,326,056 - +0.3 2,326,056 - -0.4 2,326,056 - +0.2 2,326,398 - A4249_RS11560 0.55 +1.7 2,326,398 - A4249_RS11560 0.55 -0.0 2,326,398 - A4249_RS11560 0.55 -0.2 2,326,427 + A4249_RS11560 0.60 +1.8 2,326,572 - A4249_RS11560 0.82 -0.2 2,326,704 - -0.6 2,326,704 - +0.4 2,326,704 - +0.0 2,326,727 - -0.4 2,326,766 - -0.7 2,326,980 + A4249_RS11565 0.19 -0.2 2,326,980 + A4249_RS11565 0.19 +0.3 2,326,980 + A4249_RS11565 0.19 -0.3 2,326,980 + A4249_RS11565 0.19 +0.9 2,326,981 - A4249_RS11565 0.20 +1.0 2,327,034 + A4249_RS11565 0.25 -0.0 2,327,035 - A4249_RS11565 0.25 -0.2
Or see this region's nucleotide sequence