Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS11305 and A4249_RS11310 are separated by 36 nucleotides A4249_RS11310 and A4249_RS11315 overlap by 46 nucleotides A4249_RS11315 and A4249_RS11320 are separated by 10 nucleotides
A4249_RS11305: A4249_RS11305 - bifunctional folylpolyglutamate synthase/dihydrofolate synthase, at 2,280,451 to 2,281,737
_RS11305
A4249_RS11310: A4249_RS11310 - DoxX family protein, at 2,281,774 to 2,282,217
_RS11310
A4249_RS11315: A4249_RS11315 - acetyl-CoA carboxylase, carboxyltransferase subunit beta, at 2,282,172 to 2,283,062
_RS11315
A4249_RS11320: A4249_RS11320 - tryptophan synthase subunit alpha, at 2,283,073 to 2,283,915
_RS11320
Position (kb)
2281
2282
2283 Strain fitness (log2 ratio)
-1
0
1 at 2281.715 kb on - strand at 2281.715 kb on - strand at 2281.715 kb on - strand at 2281.715 kb on - strand at 2282.052 kb on + strand, within A4249_RS11310 at 2282.052 kb on + strand, within A4249_RS11310 at 2282.052 kb on + strand, within A4249_RS11310 at 2282.053 kb on - strand, within A4249_RS11310 at 2282.053 kb on - strand, within A4249_RS11310 at 2282.053 kb on - strand, within A4249_RS11310 at 2282.083 kb on + strand, within A4249_RS11310 at 2282.083 kb on + strand, within A4249_RS11310 at 2282.083 kb on + strand, within A4249_RS11310 at 2282.084 kb on - strand, within A4249_RS11310 at 2282.084 kb on - strand, within A4249_RS11310 at 2282.084 kb on - strand, within A4249_RS11310 at 2283.075 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 2,281,715 - +0.1 2,281,715 - -0.4 2,281,715 - +0.4 2,281,715 - -0.1 2,282,052 + A4249_RS11310 0.63 -0.6 2,282,052 + A4249_RS11310 0.63 +1.5 2,282,052 + A4249_RS11310 0.63 +0.4 2,282,053 - A4249_RS11310 0.63 -0.6 2,282,053 - A4249_RS11310 0.63 -0.0 2,282,053 - A4249_RS11310 0.63 -0.9 2,282,083 + A4249_RS11310 0.70 -0.4 2,282,083 + A4249_RS11310 0.70 -0.0 2,282,083 + A4249_RS11310 0.70 -1.1 2,282,084 - A4249_RS11310 0.70 +0.1 2,282,084 - A4249_RS11310 0.70 +0.2 2,282,084 - A4249_RS11310 0.70 +1.1 2,283,075 - +0.7
Or see this region's nucleotide sequence