Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS11215 and A4249_RS11220 are separated by 56 nucleotides A4249_RS11220 and A4249_RS11225 are separated by 43 nucleotides A4249_RS11225 and A4249_RS11230 are separated by 63 nucleotides A4249_RS11230 and A4249_RS11235 are separated by 54 nucleotides A4249_RS11235 and A4249_RS11240 are separated by 12 nucleotides
A4249_RS11215: A4249_RS11215 - isoaspartyl peptidase/L-asparaginase, at 2,258,908 to 2,259,972
_RS11215
A4249_RS11220: A4249_RS11220 - hypothetical protein, at 2,260,029 to 2,260,295
_RS11220
A4249_RS11225: A4249_RS11225 - fumarylacetoacetate hydrolase family protein, at 2,260,339 to 2,261,358
_RS11225
A4249_RS11230: A4249_RS11230 - DUF1453 family protein, at 2,261,422 to 2,261,958
_RS11230
A4249_RS11235: A4249_RS11235 - tRNA-Leu, at 2,262,013 to 2,262,097
_RS11235
A4249_RS11240: A4249_RS11240 - alpha/beta hydrolase, at 2,262,110 to 2,262,961
_RS11240
Position (kb)
2260
2261
2262 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2259.682 kb on + strand, within A4249_RS11215 at 2259.682 kb on + strand, within A4249_RS11215 at 2259.682 kb on + strand, within A4249_RS11215 at 2259.683 kb on - strand, within A4249_RS11215 at 2259.683 kb on - strand, within A4249_RS11215 at 2259.683 kb on - strand, within A4249_RS11215 at 2259.683 kb on - strand, within A4249_RS11215 at 2259.733 kb on + strand, within A4249_RS11215 at 2259.733 kb on + strand, within A4249_RS11215 at 2259.734 kb on - strand, within A4249_RS11215 at 2259.734 kb on - strand, within A4249_RS11215 at 2259.734 kb on - strand, within A4249_RS11215 at 2259.910 kb on + strand at 2259.910 kb on + strand at 2259.910 kb on + strand at 2259.910 kb on + strand at 2259.910 kb on + strand at 2259.910 kb on + strand at 2259.910 kb on + strand at 2259.910 kb on + strand at 2259.911 kb on - strand at 2259.911 kb on - strand at 2259.911 kb on - strand at 2259.911 kb on - strand at 2259.911 kb on - strand at 2259.911 kb on - strand at 2259.955 kb on + strand at 2259.955 kb on + strand at 2259.955 kb on + strand at 2259.955 kb on + strand at 2259.956 kb on - strand at 2259.956 kb on - strand at 2259.991 kb on + strand at 2259.991 kb on + strand at 2259.992 kb on - strand at 2260.000 kb on + strand at 2260.000 kb on + strand at 2260.001 kb on - strand at 2260.001 kb on - strand at 2260.001 kb on - strand at 2260.001 kb on - strand at 2260.137 kb on + strand, within A4249_RS11220 at 2260.138 kb on - strand, within A4249_RS11220 at 2260.138 kb on - strand, within A4249_RS11220 at 2260.138 kb on - strand, within A4249_RS11220 at 2260.138 kb on - strand, within A4249_RS11220 at 2260.138 kb on - strand, within A4249_RS11220 at 2260.138 kb on - strand, within A4249_RS11220 at 2260.138 kb on - strand, within A4249_RS11220 at 2260.138 kb on - strand, within A4249_RS11220 at 2260.138 kb on - strand, within A4249_RS11220 at 2260.186 kb on - strand, within A4249_RS11220 at 2260.186 kb on - strand, within A4249_RS11220 at 2260.186 kb on - strand, within A4249_RS11220 at 2260.327 kb on + strand at 2260.594 kb on + strand, within A4249_RS11225 at 2260.594 kb on + strand, within A4249_RS11225 at 2260.594 kb on + strand, within A4249_RS11225 at 2260.595 kb on - strand, within A4249_RS11225 at 2260.595 kb on - strand, within A4249_RS11225 at 2260.595 kb on - strand, within A4249_RS11225 at 2260.595 kb on - strand, within A4249_RS11225 at 2260.672 kb on + strand, within A4249_RS11225 at 2260.673 kb on - strand, within A4249_RS11225 at 2260.673 kb on - strand, within A4249_RS11225 at 2260.673 kb on - strand, within A4249_RS11225 at 2261.365 kb on + strand at 2261.365 kb on + strand at 2261.366 kb on - strand at 2261.896 kb on + strand, within A4249_RS11230 at 2261.896 kb on + strand, within A4249_RS11230 at 2261.896 kb on + strand, within A4249_RS11230 at 2261.896 kb on + strand, within A4249_RS11230 at 2261.896 kb on + strand, within A4249_RS11230 at 2261.896 kb on + strand, within A4249_RS11230 at 2261.896 kb on + strand, within A4249_RS11230 at 2261.897 kb on - strand, within A4249_RS11230 at 2261.897 kb on - strand, within A4249_RS11230 at 2261.897 kb on - strand, within A4249_RS11230 at 2261.897 kb on - strand, within A4249_RS11230 at 2261.897 kb on - strand, within A4249_RS11230 at 2262.220 kb on - strand, within A4249_RS11240
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 2,259,682 + A4249_RS11215 0.73 -0.7 2,259,682 + A4249_RS11215 0.73 +0.8 2,259,682 + A4249_RS11215 0.73 -1.8 2,259,683 - A4249_RS11215 0.73 +0.1 2,259,683 - A4249_RS11215 0.73 -0.1 2,259,683 - A4249_RS11215 0.73 +0.0 2,259,683 - A4249_RS11215 0.73 +0.7 2,259,733 + A4249_RS11215 0.77 +0.3 2,259,733 + A4249_RS11215 0.77 -0.2 2,259,734 - A4249_RS11215 0.78 +0.7 2,259,734 - A4249_RS11215 0.78 -0.5 2,259,734 - A4249_RS11215 0.78 -0.4 2,259,910 + -1.2 2,259,910 + +0.1 2,259,910 + +0.4 2,259,910 + +0.6 2,259,910 + -0.1 2,259,910 + +0.3 2,259,910 + -0.0 2,259,910 + -0.7 2,259,911 - -1.8 2,259,911 - +1.0 2,259,911 - -0.9 2,259,911 - +1.2 2,259,911 - +0.9 2,259,911 - -0.0 2,259,955 + +1.9 2,259,955 + -0.4 2,259,955 + -0.4 2,259,955 + -0.5 2,259,956 - +1.1 2,259,956 - +0.4 2,259,991 + -0.9 2,259,991 + -0.9 2,259,992 - +0.6 2,260,000 + -0.1 2,260,000 + -0.1 2,260,001 - +1.7 2,260,001 - -0.3 2,260,001 - +0.3 2,260,001 - +0.6 2,260,137 + A4249_RS11220 0.40 +0.2 2,260,138 - A4249_RS11220 0.41 -0.2 2,260,138 - A4249_RS11220 0.41 -0.4 2,260,138 - A4249_RS11220 0.41 -0.4 2,260,138 - A4249_RS11220 0.41 +0.7 2,260,138 - A4249_RS11220 0.41 -0.6 2,260,138 - A4249_RS11220 0.41 -0.1 2,260,138 - A4249_RS11220 0.41 +0.1 2,260,138 - A4249_RS11220 0.41 -1.0 2,260,138 - A4249_RS11220 0.41 +0.5 2,260,186 - A4249_RS11220 0.59 -0.3 2,260,186 - A4249_RS11220 0.59 -0.1 2,260,186 - A4249_RS11220 0.59 -0.0 2,260,327 + +0.3 2,260,594 + A4249_RS11225 0.25 -0.4 2,260,594 + A4249_RS11225 0.25 -0.3 2,260,594 + A4249_RS11225 0.25 +0.9 2,260,595 - A4249_RS11225 0.25 -0.4 2,260,595 - A4249_RS11225 0.25 -0.9 2,260,595 - A4249_RS11225 0.25 +0.6 2,260,595 - A4249_RS11225 0.25 +0.6 2,260,672 + A4249_RS11225 0.33 -1.2 2,260,673 - A4249_RS11225 0.33 -0.7 2,260,673 - A4249_RS11225 0.33 +0.3 2,260,673 - A4249_RS11225 0.33 -1.3 2,261,365 + +0.0 2,261,365 + -0.3 2,261,366 - -0.3 2,261,896 + A4249_RS11230 0.88 +0.1 2,261,896 + A4249_RS11230 0.88 +0.1 2,261,896 + A4249_RS11230 0.88 +0.0 2,261,896 + A4249_RS11230 0.88 -0.4 2,261,896 + A4249_RS11230 0.88 -0.1 2,261,896 + A4249_RS11230 0.88 +0.3 2,261,896 + A4249_RS11230 0.88 +0.3 2,261,897 - A4249_RS11230 0.88 +0.3 2,261,897 - A4249_RS11230 0.88 -2.2 2,261,897 - A4249_RS11230 0.88 -0.4 2,261,897 - A4249_RS11230 0.88 +0.0 2,261,897 - A4249_RS11230 0.88 -0.2 2,262,220 - A4249_RS11240 0.13 -1.7
Or see this region's nucleotide sequence