Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS05590 and A4249_RS05595 are separated by 33 nucleotides A4249_RS05595 and A4249_RS05600 are separated by 2 nucleotides A4249_RS05600 and A4249_RS05605 overlap by 4 nucleotides
A4249_RS05590: A4249_RS05590 - amino acid ABC transporter substrate-binding protein, at 1,143,844 to 1,144,935
_RS05590
A4249_RS05595: A4249_RS05595 - dicarboxylate/amino acid:cation symporter, at 1,144,969 to 1,146,291
_RS05595
A4249_RS05600: A4249_RS05600 - 3-mercaptopyruvate sulfurtransferase, at 1,146,294 to 1,147,118
_RS05600
A4249_RS05605: A4249_RS05605 - cyclopropane-fatty-acyl-phospholipid synthase family protein, at 1,147,115 to 1,148,335
_RS05605
Position (kb)
1144
1145
1146
1147 Strain fitness (log2 ratio)
-1
0
1
2 at 1144.051 kb on + strand, within A4249_RS05590 at 1144.052 kb on - strand, within A4249_RS05590 at 1144.273 kb on + strand, within A4249_RS05590 at 1144.273 kb on + strand, within A4249_RS05590 at 1144.274 kb on - strand, within A4249_RS05590 at 1144.274 kb on - strand, within A4249_RS05590 at 1144.276 kb on + strand, within A4249_RS05590 at 1144.462 kb on + strand, within A4249_RS05590 at 1144.462 kb on + strand, within A4249_RS05590 at 1144.463 kb on - strand, within A4249_RS05590 at 1144.463 kb on - strand, within A4249_RS05590 at 1144.463 kb on - strand, within A4249_RS05590 at 1144.816 kb on + strand, within A4249_RS05590 at 1144.817 kb on - strand, within A4249_RS05590 at 1144.915 kb on + strand at 1145.289 kb on - strand, within A4249_RS05595 at 1145.379 kb on + strand, within A4249_RS05595 at 1145.692 kb on + strand, within A4249_RS05595 at 1145.693 kb on - strand, within A4249_RS05595 at 1145.693 kb on - strand, within A4249_RS05595 at 1145.693 kb on - strand, within A4249_RS05595 at 1145.693 kb on - strand, within A4249_RS05595 at 1145.693 kb on - strand, within A4249_RS05595 at 1145.693 kb on - strand, within A4249_RS05595 at 1145.741 kb on - strand, within A4249_RS05595 at 1146.042 kb on + strand, within A4249_RS05595 at 1146.123 kb on - strand, within A4249_RS05595 at 1146.352 kb on + strand at 1146.352 kb on + strand at 1146.352 kb on + strand at 1146.352 kb on + strand at 1146.352 kb on + strand at 1146.353 kb on - strand at 1146.353 kb on - strand at 1146.353 kb on - strand at 1146.353 kb on - strand at 1146.396 kb on + strand, within A4249_RS05600 at 1146.396 kb on + strand, within A4249_RS05600 at 1146.396 kb on + strand, within A4249_RS05600 at 1146.397 kb on - strand, within A4249_RS05600 at 1146.497 kb on + strand, within A4249_RS05600 at 1146.497 kb on + strand, within A4249_RS05600 at 1146.497 kb on + strand, within A4249_RS05600 at 1146.497 kb on + strand, within A4249_RS05600 at 1146.497 kb on + strand, within A4249_RS05600 at 1146.497 kb on + strand, within A4249_RS05600 at 1146.497 kb on + strand, within A4249_RS05600 at 1146.497 kb on + strand, within A4249_RS05600 at 1146.497 kb on + strand, within A4249_RS05600 at 1146.498 kb on - strand, within A4249_RS05600 at 1146.498 kb on - strand, within A4249_RS05600 at 1146.498 kb on - strand, within A4249_RS05600 at 1146.498 kb on - strand, within A4249_RS05600 at 1146.498 kb on - strand, within A4249_RS05600 at 1146.513 kb on + strand, within A4249_RS05600 at 1146.514 kb on - strand, within A4249_RS05600 at 1146.526 kb on + strand, within A4249_RS05600 at 1146.526 kb on + strand, within A4249_RS05600 at 1146.527 kb on - strand, within A4249_RS05600 at 1146.551 kb on + strand, within A4249_RS05600 at 1146.551 kb on + strand, within A4249_RS05600 at 1146.551 kb on + strand, within A4249_RS05600 at 1146.551 kb on + strand, within A4249_RS05600 at 1146.551 kb on + strand, within A4249_RS05600 at 1146.551 kb on + strand, within A4249_RS05600 at 1146.552 kb on - strand, within A4249_RS05600 at 1146.851 kb on + strand, within A4249_RS05600 at 1146.851 kb on + strand, within A4249_RS05600 at 1146.851 kb on + strand, within A4249_RS05600 at 1146.851 kb on + strand, within A4249_RS05600 at 1146.851 kb on + strand, within A4249_RS05600 at 1146.851 kb on + strand, within A4249_RS05600 at 1146.851 kb on + strand, within A4249_RS05600 at 1146.852 kb on - strand, within A4249_RS05600 at 1146.852 kb on - strand, within A4249_RS05600 at 1146.852 kb on - strand, within A4249_RS05600 at 1146.852 kb on - strand, within A4249_RS05600 at 1146.856 kb on + strand, within A4249_RS05600 at 1146.856 kb on + strand, within A4249_RS05600 at 1146.856 kb on + strand, within A4249_RS05600 at 1146.856 kb on + strand, within A4249_RS05600 at 1146.856 kb on + strand, within A4249_RS05600 at 1146.857 kb on - strand, within A4249_RS05600 at 1146.857 kb on - strand, within A4249_RS05600 at 1146.857 kb on - strand, within A4249_RS05600 at 1146.857 kb on - strand, within A4249_RS05600 at 1146.857 kb on - strand, within A4249_RS05600 at 1146.857 kb on - strand, within A4249_RS05600 at 1146.857 kb on - strand, within A4249_RS05600 at 1146.857 kb on - strand, within A4249_RS05600 at 1146.857 kb on - strand, within A4249_RS05600 at 1146.857 kb on - strand, within A4249_RS05600 at 1146.996 kb on - strand, within A4249_RS05600 at 1147.179 kb on + strand at 1147.180 kb on - strand at 1147.188 kb on + strand at 1147.188 kb on + strand at 1147.188 kb on + strand at 1147.188 kb on + strand at 1147.188 kb on + strand at 1147.188 kb on + strand at 1147.189 kb on - strand at 1147.189 kb on - strand at 1147.189 kb on - strand at 1147.189 kb on - strand at 1147.189 kb on - strand at 1147.189 kb on - strand at 1147.189 kb on - strand at 1147.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 1,144,051 + A4249_RS05590 0.19 -1.2 1,144,052 - A4249_RS05590 0.19 -1.2 1,144,273 + A4249_RS05590 0.39 +0.0 1,144,273 + A4249_RS05590 0.39 -0.3 1,144,274 - A4249_RS05590 0.39 +0.8 1,144,274 - A4249_RS05590 0.39 -1.4 1,144,276 + A4249_RS05590 0.40 +1.5 1,144,462 + A4249_RS05590 0.57 -0.9 1,144,462 + A4249_RS05590 0.57 +0.3 1,144,463 - A4249_RS05590 0.57 +0.1 1,144,463 - A4249_RS05590 0.57 +1.1 1,144,463 - A4249_RS05590 0.57 -0.7 1,144,816 + A4249_RS05590 0.89 -0.2 1,144,817 - A4249_RS05590 0.89 -0.5 1,144,915 + +0.8 1,145,289 - A4249_RS05595 0.24 -0.7 1,145,379 + A4249_RS05595 0.31 -0.2 1,145,692 + A4249_RS05595 0.55 +0.2 1,145,693 - A4249_RS05595 0.55 +0.1 1,145,693 - A4249_RS05595 0.55 +0.1 1,145,693 - A4249_RS05595 0.55 +0.1 1,145,693 - A4249_RS05595 0.55 -0.7 1,145,693 - A4249_RS05595 0.55 -1.2 1,145,693 - A4249_RS05595 0.55 -0.4 1,145,741 - A4249_RS05595 0.58 -0.1 1,146,042 + A4249_RS05595 0.81 -1.2 1,146,123 - A4249_RS05595 0.87 -0.3 1,146,352 + +0.7 1,146,352 + +0.5 1,146,352 + +0.2 1,146,352 + -0.1 1,146,352 + +0.1 1,146,353 - +0.7 1,146,353 - +0.1 1,146,353 - +0.6 1,146,353 - -0.2 1,146,396 + A4249_RS05600 0.12 +0.5 1,146,396 + A4249_RS05600 0.12 -0.6 1,146,396 + A4249_RS05600 0.12 +1.3 1,146,397 - A4249_RS05600 0.12 -0.9 1,146,497 + A4249_RS05600 0.25 +0.4 1,146,497 + A4249_RS05600 0.25 -0.3 1,146,497 + A4249_RS05600 0.25 -0.5 1,146,497 + A4249_RS05600 0.25 +0.3 1,146,497 + A4249_RS05600 0.25 -1.2 1,146,497 + A4249_RS05600 0.25 +0.1 1,146,497 + A4249_RS05600 0.25 +1.2 1,146,497 + A4249_RS05600 0.25 -0.4 1,146,497 + A4249_RS05600 0.25 -0.1 1,146,498 - A4249_RS05600 0.25 +0.1 1,146,498 - A4249_RS05600 0.25 +1.4 1,146,498 - A4249_RS05600 0.25 -0.9 1,146,498 - A4249_RS05600 0.25 +0.8 1,146,498 - A4249_RS05600 0.25 -0.6 1,146,513 + A4249_RS05600 0.27 -0.1 1,146,514 - A4249_RS05600 0.27 -0.5 1,146,526 + A4249_RS05600 0.28 +0.2 1,146,526 + A4249_RS05600 0.28 -1.3 1,146,527 - A4249_RS05600 0.28 -0.1 1,146,551 + A4249_RS05600 0.31 +0.1 1,146,551 + A4249_RS05600 0.31 -0.1 1,146,551 + A4249_RS05600 0.31 -0.1 1,146,551 + A4249_RS05600 0.31 +0.3 1,146,551 + A4249_RS05600 0.31 -0.4 1,146,551 + A4249_RS05600 0.31 +0.7 1,146,552 - A4249_RS05600 0.31 -0.1 1,146,851 + A4249_RS05600 0.68 +0.3 1,146,851 + A4249_RS05600 0.68 +2.2 1,146,851 + A4249_RS05600 0.68 -1.0 1,146,851 + A4249_RS05600 0.68 +0.7 1,146,851 + A4249_RS05600 0.68 +0.1 1,146,851 + A4249_RS05600 0.68 +1.1 1,146,851 + A4249_RS05600 0.68 +0.0 1,146,852 - A4249_RS05600 0.68 -0.8 1,146,852 - A4249_RS05600 0.68 +0.5 1,146,852 - A4249_RS05600 0.68 +0.2 1,146,852 - A4249_RS05600 0.68 +0.5 1,146,856 + A4249_RS05600 0.68 -0.3 1,146,856 + A4249_RS05600 0.68 +0.1 1,146,856 + A4249_RS05600 0.68 +0.7 1,146,856 + A4249_RS05600 0.68 +1.4 1,146,856 + A4249_RS05600 0.68 +0.5 1,146,857 - A4249_RS05600 0.68 -0.4 1,146,857 - A4249_RS05600 0.68 -1.0 1,146,857 - A4249_RS05600 0.68 +0.4 1,146,857 - A4249_RS05600 0.68 +1.4 1,146,857 - A4249_RS05600 0.68 -0.1 1,146,857 - A4249_RS05600 0.68 -0.5 1,146,857 - A4249_RS05600 0.68 +0.9 1,146,857 - A4249_RS05600 0.68 -0.5 1,146,857 - A4249_RS05600 0.68 +0.3 1,146,857 - A4249_RS05600 0.68 +0.6 1,146,996 - A4249_RS05600 0.85 -0.4 1,147,179 + +0.1 1,147,180 - +1.8 1,147,188 + -0.9 1,147,188 + +0.5 1,147,188 + +0.4 1,147,188 + +0.5 1,147,188 + +0.6 1,147,188 + -0.1 1,147,189 - +0.0 1,147,189 - +0.3 1,147,189 - +0.8 1,147,189 - +0.2 1,147,189 - +0.3 1,147,189 - -0.0 1,147,189 - +0.5 1,147,189 - -0.7
Or see this region's nucleotide sequence