Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS02310 and A4249_RS02315 are separated by 221 nucleotides A4249_RS02315 and A4249_RS02320 are separated by 83 nucleotides A4249_RS02320 and A4249_RS02325 are separated by 200 nucleotides A4249_RS02325 and A4249_RS02330 are separated by 87 nucleotides A4249_RS02330 and A4249_RS02335 are separated by 87 nucleotides A4249_RS02335 and A4249_RS02340 are separated by 172 nucleotides
A4249_RS02310: A4249_RS02310 - 16S ribosomal RNA, at 488,282 to 489,741
_RS02310
A4249_RS02315: A4249_RS02315 - tRNA-Ile, at 489,963 to 490,039
_RS02315
A4249_RS02320: A4249_RS02320 - tRNA-Ala, at 490,123 to 490,198
_RS02320
A4249_RS02325: A4249_RS02325 - 23S ribosomal RNA, at 490,399 to 493,177
_RS02325
A4249_RS02330: A4249_RS02330 - 5S ribosomal RNA, at 493,265 to 493,379
_RS02330
A4249_RS02335: A4249_RS02335 - tRNA-Met, at 493,467 to 493,543
_RS02335
A4249_RS02340: A4249_RS02340 - helicase-related protein, at 493,716 to 497,021
_RS02340
Position (kb)
490
491
492
493
494 Strain fitness (log2 ratio)
-1
0
1 at 491.424 kb on + strand, within A4249_RS02325 at 491.445 kb on - strand, within A4249_RS02325 at 491.850 kb on + strand, within A4249_RS02325 at 491.865 kb on + strand, within A4249_RS02325 at 491.865 kb on + strand, within A4249_RS02325 at 493.611 kb on - strand at 493.638 kb on - strand at 493.638 kb on - strand at 493.638 kb on - strand at 494.062 kb on + strand, within A4249_RS02340 at 494.062 kb on + strand, within A4249_RS02340 at 494.062 kb on + strand, within A4249_RS02340 at 494.062 kb on + strand, within A4249_RS02340 at 494.062 kb on + strand, within A4249_RS02340 at 494.062 kb on + strand, within A4249_RS02340 at 494.063 kb on - strand, within A4249_RS02340
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 491,424 + A4249_RS02325 0.37 -0.9 491,445 - A4249_RS02325 0.38 +0.1 491,850 + A4249_RS02325 0.52 -1.0 491,865 + A4249_RS02325 0.53 -0.0 491,865 + A4249_RS02325 0.53 -1.2 493,611 - -0.3 493,638 - +0.3 493,638 - -0.7 493,638 - -0.4 494,062 + A4249_RS02340 0.10 +0.2 494,062 + A4249_RS02340 0.10 -0.1 494,062 + A4249_RS02340 0.10 +0.1 494,062 + A4249_RS02340 0.10 +0.3 494,062 + A4249_RS02340 0.10 -0.8 494,062 + A4249_RS02340 0.10 -0.4 494,063 - A4249_RS02340 0.10 -1.7
Or see this region's nucleotide sequence