Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS01340 and A4249_RS01345 overlap by 4 nucleotides A4249_RS01345 and A4249_RS01350 are separated by 13 nucleotides A4249_RS01350 and A4249_RS01355 are separated by 166 nucleotides A4249_RS01355 and A4249_RS01360 are separated by 34 nucleotides A4249_RS01360 and A4249_RS01365 overlap by 4 nucleotides A4249_RS01365 and A4249_RS01370 overlap by 4 nucleotides
A4249_RS01340: A4249_RS01340 - phosphoadenosine phosphosulfate reductase family protein, at 254,334 to 255,479
_RS01340
A4249_RS01345: A4249_RS01345 - helix-turn-helix domain-containing protein, at 255,476 to 255,892
_RS01345
A4249_RS01350: A4249_RS01350 - hypothetical protein, at 255,906 to 256,256
_RS01350
A4249_RS01355: A4249_RS01355 - hypothetical protein, at 256,423 to 257,073
_RS01355
A4249_RS01360: A4249_RS01360 - hypothetical protein, at 257,108 to 257,509
_RS01360
A4249_RS01365: A4249_RS01365 - hypothetical protein, at 257,506 to 257,952
_RS01365
A4249_RS01370: A4249_RS01370 - DUF6525 family protein, at 257,949 to 258,197
_RS01370
Position (kb) 256
257
258 Strain fitness (log2 ratio) -1
0
1 at 255.561 kb on + strand, within A4249_RS01345 at 256.335 kb on + strand at 256.335 kb on + strand at 256.335 kb on + strand at 256.335 kb on + strand at 256.335 kb on + strand at 256.335 kb on + strand at 256.410 kb on + strand at 256.410 kb on + strand at 256.410 kb on + strand at 256.410 kb on + strand at 256.410 kb on + strand at 256.734 kb on + strand, within A4249_RS01355 at 256.734 kb on + strand, within A4249_RS01355 at 257.075 kb on + strand at 257.075 kb on + strand at 257.076 kb on - strand at 257.076 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 255,561 + A4249_RS01345 0.20 +1.0 256,335 + +1.0 256,335 + +0.1 256,335 + -1.6 256,335 + -0.4 256,335 + -0.7 256,335 + -0.0 256,410 + -0.6 256,410 + -0.6 256,410 + +0.1 256,410 + -0.3 256,410 + +0.3 256,734 + A4249_RS01355 0.48 -0.9 256,734 + A4249_RS01355 0.48 -0.1 257,075 + +0.5 257,075 + +0.2 257,076 - -0.7 257,076 - -1.5
Or see this region's nucleotide sequence