Strain Fitness in Escherichia coli BW25113 around b0001

Experiment: D-Glucose (C) M9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrL and thrA are separated by 81 nucleotides b0001: thrL - thr operon leader peptide (NCBI), at 190 to 255 thrL b0002: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI), at 337 to 2,799 thrA Position (kb) 0 1Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 0.070 kb on + strandat 0.070 kb on + strandat 0.078 kb on - strandat 0.078 kb on - strandat 0.161 kb on - strandat 0.185 kb on - strandat 0.185 kb on - strandat 0.208 kb on + strand, within thrLat 0.217 kb on - strand, within thrLat 0.257 kb on - strandat 0.257 kb on - strandat 0.440 kb on - strandat 0.440 kb on - strandat 0.454 kb on - strandat 0.518 kb on - strandat 0.518 kb on - strandat 0.528 kb on + strandat 0.592 kb on + strand, within thrAat 0.592 kb on + strand, within thrAat 0.666 kb on + strand, within thrAat 0.666 kb on + strand, within thrAat 0.675 kb on - strand, within thrAat 0.754 kb on - strand, within thrAat 0.754 kb on - strand, within thrAat 0.767 kb on + strand, within thrAat 0.775 kb on - strand, within thrAat 0.957 kb on + strand, within thrAat 0.957 kb on + strand, within thrAat 1.046 kb on + strand, within thrAat 1.136 kb on - strand, within thrAat 1.142 kb on - strand, within thrAat 1.161 kb on + strand, within thrAat 1.161 kb on + strand, within thrAat 1.161 kb on + strand, within thrA

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C) M9
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70 + -1.5
70 + -0.3
78 - +0.6
78 - -0.1
161 - -2.3
185 - -1.7
185 - -1.1
208 + thrL b0001 0.27 -1.3
217 - thrL b0001 0.41 -2.9
257 - +0.9
257 - -2.6
440 - -3.5
440 - -0.9
454 - -2.6
518 - -2.3
518 - -0.7
528 + -1.9
592 + thrA b0002 0.10 -0.4
592 + thrA b0002 0.10 -0.7
666 + thrA b0002 0.13 -1.1
666 + thrA b0002 0.13 -1.9
675 - thrA b0002 0.14 -2.6
754 - thrA b0002 0.17 -3.2
754 - thrA b0002 0.17 -0.9
767 + thrA b0002 0.17 -1.6
775 - thrA b0002 0.18 -2.1
957 + thrA b0002 0.25 -1.7
957 + thrA b0002 0.25 -0.1
1,046 + thrA b0002 0.29 -2.7
1,136 - thrA b0002 0.32 -3.4
1,142 - thrA b0002 0.33 -4.1
1,161 + thrA b0002 0.33 -1.9
1,161 + thrA b0002 0.33 -2.9
1,161 + thrA b0002 0.33 -2.8

Or see this region's nucleotide sequence