Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS01110 and ABID97_RS01115 are separated by 46 nucleotides ABID97_RS01115 and ABID97_RS01120 overlap by 2 nucleotides ABID97_RS01120 and ABID97_RS01125 are separated by 24 nucleotides ABID97_RS01125 and ABID97_RS01130 are separated by 290 nucleotides
ABID97_RS01110: ABID97_RS01110 - citrate/2-methylcitrate synthase, at 224,746 to 225,000
_RS01110
ABID97_RS01115: ABID97_RS01115 - citrate/2-methylcitrate synthase, at 225,047 to 225,946
_RS01115
ABID97_RS01120: ABID97_RS01120 - citrate synthase, at 225,945 to 227,111
_RS01120
ABID97_RS01125: ABID97_RS01125 - type 1 glutamine amidotransferase domain-containing protein, at 227,136 to 227,708
_RS01125
ABID97_RS01130: ABID97_RS01130 - tripartite tricarboxylate transporter substrate-binding protein, at 227,999 to 228,973
_RS01130
Position (kb) 225
226
227
228 Strain fitness (log2 ratio) -1
0
1
2 at 225.109 kb on - strand at 225.361 kb on - strand, within ABID97_RS01115 at 225.873 kb on + strand at 225.913 kb on + strand at 225.914 kb on - strand at 226.086 kb on + strand, within ABID97_RS01120 at 226.825 kb on - strand, within ABID97_RS01120 at 227.749 kb on + strand at 227.750 kb on - strand at 227.750 kb on - strand at 227.827 kb on + strand at 227.828 kb on - strand at 227.847 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 225,109 - -0.1 225,361 - ABID97_RS01115 0.35 -1.0 225,873 + -1.4 225,913 + +0.1 225,914 - -0.1 226,086 + ABID97_RS01120 0.12 -1.5 226,825 - ABID97_RS01120 0.75 -1.0 227,749 + +1.1 227,750 - -0.8 227,750 - -0.4 227,827 + +1.8 227,828 - +0.2 227,847 + +0.3
Or see this region's nucleotide sequence