Experiment: D-Galacturonic Acid (C) M9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sstT and ygjV are separated by 4 nucleotides ygjV and uxaA are separated by 82 nucleotides
b3089: sstT - sodium:serine/threonine symporter (NCBI), at 3,237,966 to 3,239,210
sstT
b3090: ygjV - conserved inner membrane protein (NCBI), at 3,239,215 to 3,239,766
ygjV
b3091: uxaA - altronate hydrolase (NCBI), at 3,239,849 to 3,241,336
uxaA
Position (kb)
3239
3240 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3238.232 kb on + strand, within sstT at 3238.233 kb on - strand, within sstT at 3238.233 kb on - strand, within sstT at 3238.246 kb on + strand, within sstT at 3238.259 kb on - strand, within sstT at 3238.259 kb on - strand, within sstT at 3238.291 kb on - strand, within sstT at 3238.357 kb on + strand, within sstT at 3238.365 kb on - strand, within sstT at 3238.374 kb on + strand, within sstT at 3238.395 kb on + strand, within sstT at 3238.397 kb on + strand, within sstT at 3238.419 kb on + strand, within sstT at 3238.430 kb on - strand, within sstT at 3238.430 kb on - strand, within sstT at 3238.484 kb on + strand, within sstT at 3238.484 kb on + strand, within sstT at 3238.492 kb on - strand, within sstT at 3238.607 kb on - strand, within sstT at 3238.607 kb on - strand, within sstT at 3238.647 kb on - strand, within sstT at 3238.702 kb on + strand, within sstT at 3238.717 kb on + strand, within sstT at 3238.717 kb on + strand, within sstT at 3238.740 kb on + strand, within sstT at 3238.748 kb on - strand, within sstT at 3238.748 kb on - strand, within sstT at 3238.748 kb on - strand, within sstT at 3238.751 kb on - strand, within sstT at 3238.792 kb on - strand, within sstT at 3238.824 kb on + strand, within sstT at 3238.825 kb on + strand, within sstT at 3238.878 kb on + strand, within sstT at 3238.878 kb on + strand, within sstT at 3238.917 kb on - strand, within sstT at 3238.937 kb on + strand, within sstT at 3238.949 kb on + strand, within sstT at 3239.032 kb on + strand, within sstT at 3239.040 kb on - strand, within sstT at 3239.040 kb on - strand, within sstT at 3239.064 kb on - strand, within sstT at 3239.064 kb on - strand, within sstT at 3239.088 kb on + strand at 3239.100 kb on + strand at 3239.100 kb on + strand at 3239.102 kb on - strand at 3239.102 kb on - strand at 3239.152 kb on - strand at 3239.152 kb on - strand at 3239.348 kb on + strand, within ygjV at 3239.360 kb on + strand, within ygjV at 3239.368 kb on + strand, within ygjV at 3239.400 kb on + strand, within ygjV at 3239.400 kb on + strand, within ygjV at 3239.424 kb on - strand, within ygjV at 3239.487 kb on + strand, within ygjV at 3239.526 kb on - strand, within ygjV at 3239.544 kb on + strand, within ygjV at 3239.544 kb on + strand, within ygjV at 3239.552 kb on - strand, within ygjV at 3239.585 kb on + strand, within ygjV at 3239.585 kb on + strand, within ygjV at 3239.653 kb on + strand, within ygjV at 3239.741 kb on + strand at 3239.741 kb on + strand at 3239.807 kb on + strand at 3239.899 kb on - strand at 3239.899 kb on - strand at 3239.937 kb on - strand at 3239.973 kb on + strand at 3239.986 kb on - strand at 3240.082 kb on - strand, within uxaA at 3240.094 kb on - strand, within uxaA at 3240.094 kb on - strand, within uxaA at 3240.096 kb on + strand, within uxaA at 3240.096 kb on + strand, within uxaA at 3240.099 kb on - strand, within uxaA at 3240.106 kb on + strand, within uxaA at 3240.114 kb on - strand, within uxaA at 3240.128 kb on + strand, within uxaA at 3240.166 kb on + strand, within uxaA at 3240.166 kb on + strand, within uxaA at 3240.176 kb on + strand, within uxaA at 3240.176 kb on + strand, within uxaA at 3240.255 kb on - strand, within uxaA at 3240.262 kb on - strand, within uxaA at 3240.277 kb on + strand, within uxaA at 3240.277 kb on + strand, within uxaA at 3240.277 kb on + strand, within uxaA at 3240.281 kb on + strand, within uxaA at 3240.347 kb on - strand, within uxaA at 3240.347 kb on - strand, within uxaA at 3240.347 kb on - strand, within uxaA at 3240.359 kb on + strand, within uxaA at 3240.359 kb on + strand, within uxaA at 3240.415 kb on - strand, within uxaA at 3240.415 kb on - strand, within uxaA at 3240.454 kb on + strand, within uxaA at 3240.454 kb on + strand, within uxaA at 3240.470 kb on - strand, within uxaA at 3240.556 kb on - strand, within uxaA at 3240.614 kb on - strand, within uxaA at 3240.661 kb on - strand, within uxaA at 3240.661 kb on - strand, within uxaA at 3240.743 kb on + strand, within uxaA at 3240.743 kb on + strand, within uxaA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galacturonic Acid (C) M9 remove 3,238,232 + sstT b3089 0.21 -1.0 3,238,233 - sstT b3089 0.21 -1.0 3,238,233 - sstT b3089 0.21 +0.4 3,238,246 + sstT b3089 0.22 +0.5 3,238,259 - sstT b3089 0.24 +1.2 3,238,259 - sstT b3089 0.24 -0.7 3,238,291 - sstT b3089 0.26 -0.1 3,238,357 + sstT b3089 0.31 +0.5 3,238,365 - sstT b3089 0.32 -0.4 3,238,374 + sstT b3089 0.33 -0.2 3,238,395 + sstT b3089 0.34 -0.6 3,238,397 + sstT b3089 0.35 -0.0 3,238,419 + sstT b3089 0.36 +0.7 3,238,430 - sstT b3089 0.37 -0.6 3,238,430 - sstT b3089 0.37 -0.2 3,238,484 + sstT b3089 0.42 -0.4 3,238,484 + sstT b3089 0.42 +1.0 3,238,492 - sstT b3089 0.42 -0.6 3,238,607 - sstT b3089 0.51 -0.0 3,238,607 - sstT b3089 0.51 +0.6 3,238,647 - sstT b3089 0.55 -0.1 3,238,702 + sstT b3089 0.59 -0.5 3,238,717 + sstT b3089 0.60 +0.7 3,238,717 + sstT b3089 0.60 +0.6 3,238,740 + sstT b3089 0.62 +0.5 3,238,748 - sstT b3089 0.63 +0.7 3,238,748 - sstT b3089 0.63 -0.4 3,238,748 - sstT b3089 0.63 +0.0 3,238,751 - sstT b3089 0.63 +1.0 3,238,792 - sstT b3089 0.66 +0.2 3,238,824 + sstT b3089 0.69 -0.7 3,238,825 + sstT b3089 0.69 -0.1 3,238,878 + sstT b3089 0.73 +0.8 3,238,878 + sstT b3089 0.73 +0.5 3,238,917 - sstT b3089 0.76 -0.2 3,238,937 + sstT b3089 0.78 -0.4 3,238,949 + sstT b3089 0.79 +0.1 3,239,032 + sstT b3089 0.86 +1.7 3,239,040 - sstT b3089 0.86 +1.0 3,239,040 - sstT b3089 0.86 +0.7 3,239,064 - sstT b3089 0.88 -0.6 3,239,064 - sstT b3089 0.88 +1.9 3,239,088 + +0.6 3,239,100 + +0.5 3,239,100 + -0.8 3,239,102 - +0.2 3,239,102 - +0.2 3,239,152 - +0.6 3,239,152 - +0.6 3,239,348 + ygjV b3090 0.24 +0.3 3,239,360 + ygjV b3090 0.26 -1.1 3,239,368 + ygjV b3090 0.28 -0.2 3,239,400 + ygjV b3090 0.34 +0.1 3,239,400 + ygjV b3090 0.34 -0.8 3,239,424 - ygjV b3090 0.38 +0.2 3,239,487 + ygjV b3090 0.49 +0.2 3,239,526 - ygjV b3090 0.56 +0.1 3,239,544 + ygjV b3090 0.60 +0.6 3,239,544 + ygjV b3090 0.60 -0.2 3,239,552 - ygjV b3090 0.61 +0.1 3,239,585 + ygjV b3090 0.67 -0.6 3,239,585 + ygjV b3090 0.67 +0.3 3,239,653 + ygjV b3090 0.79 +0.2 3,239,741 + -0.0 3,239,741 + +0.2 3,239,807 + -0.8 3,239,899 - -2.0 3,239,899 - -2.7 3,239,937 - -3.6 3,239,973 + -1.3 3,239,986 - -2.0 3,240,082 - uxaA b3091 0.16 -4.8 3,240,094 - uxaA b3091 0.16 -3.4 3,240,094 - uxaA b3091 0.16 -3.2 3,240,096 + uxaA b3091 0.17 -2.8 3,240,096 + uxaA b3091 0.17 -3.2 3,240,099 - uxaA b3091 0.17 -0.6 3,240,106 + uxaA b3091 0.17 -1.6 3,240,114 - uxaA b3091 0.18 -1.7 3,240,128 + uxaA b3091 0.19 -4.1 3,240,166 + uxaA b3091 0.21 -1.6 3,240,166 + uxaA b3091 0.21 +1.0 3,240,176 + uxaA b3091 0.22 -4.6 3,240,176 + uxaA b3091 0.22 -4.8 3,240,255 - uxaA b3091 0.27 -3.1 3,240,262 - uxaA b3091 0.28 -2.2 3,240,277 + uxaA b3091 0.29 -2.0 3,240,277 + uxaA b3091 0.29 -2.3 3,240,277 + uxaA b3091 0.29 -3.2 3,240,281 + uxaA b3091 0.29 -2.6 3,240,347 - uxaA b3091 0.33 -1.8 3,240,347 - uxaA b3091 0.33 +1.0 3,240,347 - uxaA b3091 0.33 -1.8 3,240,359 + uxaA b3091 0.34 -1.8 3,240,359 + uxaA b3091 0.34 -3.1 3,240,415 - uxaA b3091 0.38 -2.9 3,240,415 - uxaA b3091 0.38 -1.8 3,240,454 + uxaA b3091 0.41 -2.8 3,240,454 + uxaA b3091 0.41 -2.7 3,240,470 - uxaA b3091 0.42 -2.2 3,240,556 - uxaA b3091 0.48 -2.0 3,240,614 - uxaA b3091 0.51 -2.7 3,240,661 - uxaA b3091 0.55 -2.3 3,240,661 - uxaA b3091 0.55 -1.3 3,240,743 + uxaA b3091 0.60 -3.3 3,240,743 + uxaA b3091 0.60 -3.2
Or see this region's nucleotide sequence