Strain Fitness in Escherichia coli BW25113 around b3995

Experiment: D-Glucose (C) MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiC and rsd are separated by 232 nucleotidesrsd and nudC are separated by 94 nucleotidesnudC and hemE are separated by 39 nucleotides b3994: thiC - thiamine biosynthesis protein ThiC (NCBI), at 4,192,227 to 4,194,122 thiC b3995: rsd - stationary phase protein, binds sigma 70 RNA polymerase subunit (NCBI), at 4,194,355 to 4,194,831 rsd b3996: nudC - NADH pyrophosphatase (NCBI), at 4,194,926 to 4,195,699 nudC b3997: hemE - uroporphyrinogen decarboxylase (NCBI), at 4,195,739 to 4,196,803 hemE Position (kb) 4194 4195Strain fitness (log2 ratio) -3 -2 -1 0 1at 4193.598 kb on - strand, within thiCat 4193.678 kb on - strand, within thiCat 4193.678 kb on - strand, within thiCat 4193.679 kb on + strand, within thiCat 4193.679 kb on + strand, within thiCat 4193.714 kb on + strand, within thiCat 4193.756 kb on - strand, within thiCat 4193.756 kb on - strand, within thiCat 4193.793 kb on + strand, within thiCat 4193.890 kb on + strand, within thiCat 4193.896 kb on - strand, within thiCat 4193.952 kb on + strandat 4193.970 kb on + strandat 4193.980 kb on - strandat 4193.980 kb on - strandat 4194.014 kb on - strandat 4194.054 kb on + strandat 4194.061 kb on - strandat 4194.061 kb on - strandat 4194.118 kb on + strandat 4194.164 kb on + strandat 4194.165 kb on + strandat 4194.165 kb on + strandat 4194.173 kb on - strandat 4194.515 kb on - strand, within rsdat 4194.516 kb on + strand, within rsdat 4194.602 kb on - strand, within rsdat 4194.602 kb on - strand, within rsdat 4194.726 kb on + strand, within rsdat 4194.726 kb on + strand, within rsdat 4194.728 kb on - strand, within rsdat 4194.728 kb on - strand, within rsdat 4194.732 kb on + strand, within rsdat 4194.732 kb on + strand, within rsdat 4194.738 kb on - strand, within rsdat 4194.751 kb on + strand, within rsdat 4194.755 kb on + strand, within rsdat 4194.812 kb on - strandat 4194.822 kb on + strandat 4194.905 kb on + strandat 4194.911 kb on - strandat 4194.923 kb on + strandat 4194.923 kb on + strandat 4194.965 kb on + strandat 4195.102 kb on - strand, within nudCat 4195.102 kb on - strand, within nudCat 4195.124 kb on - strand, within nudCat 4195.125 kb on + strand, within nudCat 4195.143 kb on - strand, within nudCat 4195.178 kb on + strand, within nudCat 4195.178 kb on + strand, within nudCat 4195.216 kb on - strand, within nudCat 4195.216 kb on - strand, within nudCat 4195.218 kb on + strand, within nudCat 4195.218 kb on + strand, within nudCat 4195.260 kb on + strand, within nudCat 4195.260 kb on + strand, within nudCat 4195.270 kb on + strand, within nudCat 4195.278 kb on - strand, within nudCat 4195.296 kb on - strand, within nudCat 4195.305 kb on - strand, within nudCat 4195.312 kb on + strand, within nudCat 4195.312 kb on + strand, within nudCat 4195.315 kb on - strand, within nudCat 4195.355 kb on + strand, within nudCat 4195.380 kb on - strand, within nudCat 4195.386 kb on + strand, within nudCat 4195.389 kb on - strand, within nudCat 4195.391 kb on - strand, within nudCat 4195.391 kb on - strand, within nudCat 4195.394 kb on - strand, within nudCat 4195.448 kb on - strand, within nudCat 4195.448 kb on - strand, within nudCat 4195.449 kb on + strand, within nudCat 4195.457 kb on - strand, within nudCat 4195.457 kb on - strand, within nudCat 4195.472 kb on - strand, within nudCat 4195.494 kb on + strand, within nudCat 4195.494 kb on - strand, within nudCat 4195.607 kb on - strand, within nudCat 4195.619 kb on + strand, within nudCat 4195.619 kb on + strand, within nudCat 4195.641 kb on - strandat 4195.641 kb on - strandat 4195.651 kb on - strandat 4195.661 kb on - strandat 4195.661 kb on - strandat 4195.665 kb on + strandat 4195.670 kb on + strandat 4195.680 kb on + strandat 4195.686 kb on - strandat 4195.688 kb on + strandat 4195.688 kb on + strandat 4195.688 kb on + strandat 4195.690 kb on + strandat 4195.699 kb on + strandat 4195.699 kb on + strandat 4195.699 kb on + strandat 4195.707 kb on - strandat 4195.707 kb on - strandat 4195.707 kb on - strandat 4195.707 kb on - strandat 4195.707 kb on - strandat 4195.707 kb on - strandat 4195.707 kb on - strandat 4195.709 kb on - strandat 4195.723 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C) MOPS
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4,193,598 - thiC b3994 0.72 -0.6
4,193,678 - thiC b3994 0.77 +0.2
4,193,678 - thiC b3994 0.77 -1.8
4,193,679 + thiC b3994 0.77 -1.6
4,193,679 + thiC b3994 0.77 -2.7
4,193,714 + thiC b3994 0.78 -0.2
4,193,756 - thiC b3994 0.81 -0.8
4,193,756 - thiC b3994 0.81 -1.6
4,193,793 + thiC b3994 0.83 +0.2
4,193,890 + thiC b3994 0.88 -2.2
4,193,896 - thiC b3994 0.88 -1.8
4,193,952 + -2.4
4,193,970 + -0.4
4,193,980 - -2.3
4,193,980 - -1.2
4,194,014 - -2.0
4,194,054 + +0.2
4,194,061 - -2.1
4,194,061 - -1.3
4,194,118 + -1.7
4,194,164 + -1.2
4,194,165 + -3.2
4,194,165 + -2.6
4,194,173 - +0.6
4,194,515 - rsd b3995 0.34 -0.0
4,194,516 + rsd b3995 0.34 -0.4
4,194,602 - rsd b3995 0.52 +0.9
4,194,602 - rsd b3995 0.52 -0.8
4,194,726 + rsd b3995 0.78 +0.4
4,194,726 + rsd b3995 0.78 -1.3
4,194,728 - rsd b3995 0.78 +0.9
4,194,728 - rsd b3995 0.78 -0.3
4,194,732 + rsd b3995 0.79 -0.4
4,194,732 + rsd b3995 0.79 -0.7
4,194,738 - rsd b3995 0.80 -0.5
4,194,751 + rsd b3995 0.83 +1.6
4,194,755 + rsd b3995 0.84 -1.3
4,194,812 - -0.0
4,194,822 + +0.1
4,194,905 + +0.0
4,194,911 - -0.2
4,194,923 + -1.2
4,194,923 + -0.6
4,194,965 + -0.0
4,195,102 - nudC b3996 0.23 -0.1
4,195,102 - nudC b3996 0.23 -0.8
4,195,124 - nudC b3996 0.26 +0.7
4,195,125 + nudC b3996 0.26 -0.9
4,195,143 - nudC b3996 0.28 +0.2
4,195,178 + nudC b3996 0.33 +0.3
4,195,178 + nudC b3996 0.33 -0.6
4,195,216 - nudC b3996 0.37 +0.3
4,195,216 - nudC b3996 0.37 +1.2
4,195,218 + nudC b3996 0.38 -1.4
4,195,218 + nudC b3996 0.38 -1.0
4,195,260 + nudC b3996 0.43 -0.4
4,195,260 + nudC b3996 0.43 +0.1
4,195,270 + nudC b3996 0.44 -0.8
4,195,278 - nudC b3996 0.45 -0.8
4,195,296 - nudC b3996 0.48 -0.1
4,195,305 - nudC b3996 0.49 -0.8
4,195,312 + nudC b3996 0.50 -0.2
4,195,312 + nudC b3996 0.50 -0.4
4,195,315 - nudC b3996 0.50 +1.3
4,195,355 + nudC b3996 0.55 +1.2
4,195,380 - nudC b3996 0.59 +1.0
4,195,386 + nudC b3996 0.59 -0.1
4,195,389 - nudC b3996 0.60 +1.2
4,195,391 - nudC b3996 0.60 +0.3
4,195,391 - nudC b3996 0.60 -0.2
4,195,394 - nudC b3996 0.60 +0.1
4,195,448 - nudC b3996 0.67 -0.2
4,195,448 - nudC b3996 0.67 +0.6
4,195,449 + nudC b3996 0.68 -0.4
4,195,457 - nudC b3996 0.69 +0.2
4,195,457 - nudC b3996 0.69 +0.2
4,195,472 - nudC b3996 0.71 +0.2
4,195,494 + nudC b3996 0.73 -0.3
4,195,494 - nudC b3996 0.73 -0.3
4,195,607 - nudC b3996 0.88 -0.2
4,195,619 + nudC b3996 0.90 +0.3
4,195,619 + nudC b3996 0.90 +1.0
4,195,641 - -0.3
4,195,641 - +0.2
4,195,651 - +0.3
4,195,661 - +0.4
4,195,661 - +0.6
4,195,665 + +0.4
4,195,670 + -0.3
4,195,680 + +0.1
4,195,686 - -1.5
4,195,688 + -0.1
4,195,688 + -0.6
4,195,688 + +0.2
4,195,690 + +0.2
4,195,699 + +0.5
4,195,699 + -1.8
4,195,699 + -0.6
4,195,707 - +1.6
4,195,707 - +0.4
4,195,707 - +0.3
4,195,707 - +0.2
4,195,707 - -0.4
4,195,707 - +1.6
4,195,707 - -0.3
4,195,709 - +1.2
4,195,723 - +1.6

Or see this region's nucleotide sequence