Strain Fitness in Escherichia coli BW25113 around b2314

Experiment: D-Galacturonic Acid (C) MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpurF and cvpA are separated by 36 nucleotidescvpA and dedD are separated by 258 nucleotidesdedD and folC overlap by 11 nucleotides b2312: purF - amidophosphoribosyltransferase (NCBI), at 2,426,743 to 2,428,260 purF b2313: cvpA - membrane protein required for colicin V production (NCBI), at 2,428,297 to 2,428,785 cvpA b2314: dedD - putative lipoprotein (VIMSS), at 2,429,044 to 2,429,706 dedD b2315: folC - bifunctional folylpolyglutamate synthase/ dihydrofolate synthase (NCBI), at 2,429,696 to 2,430,964 folC Position (kb) 2429 2430Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2428.063 kb on + strand, within purFat 2428.064 kb on - strand, within purFat 2428.064 kb on - strand, within purFat 2428.146 kb on - strandat 2428.157 kb on - strandat 2428.194 kb on + strandat 2428.194 kb on + strandat 2428.202 kb on - strandat 2428.202 kb on - strandat 2428.203 kb on - strandat 2428.385 kb on - strand, within cvpAat 2428.458 kb on - strand, within cvpAat 2428.510 kb on - strand, within cvpAat 2428.649 kb on + strand, within cvpAat 2428.657 kb on - strand, within cvpAat 2428.657 kb on - strand, within cvpAat 2428.684 kb on - strand, within cvpAat 2428.723 kb on + strand, within cvpAat 2428.775 kb on + strandat 2428.775 kb on + strandat 2428.816 kb on - strandat 2428.818 kb on - strandat 2428.818 kb on - strandat 2428.986 kb on - strandat 2429.015 kb on - strandat 2429.016 kb on + strandat 2429.072 kb on - strandat 2429.111 kb on - strand, within dedDat 2429.281 kb on - strand, within dedDat 2429.281 kb on - strand, within dedDat 2429.287 kb on - strand, within dedDat 2429.461 kb on - strand, within dedDat 2429.480 kb on + strand, within dedDat 2429.596 kb on - strand, within dedDat 2429.596 kb on - strand, within dedDat 2429.646 kb on - strandat 2429.646 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Galacturonic Acid (C) MOPS
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2,428,063 + purF b2312 0.87 -2.8
2,428,064 - purF b2312 0.87 -2.2
2,428,064 - purF b2312 0.87 -1.4
2,428,146 - -3.8
2,428,157 - -3.8
2,428,194 + -3.2
2,428,194 + -3.5
2,428,202 - -1.7
2,428,202 - -1.7
2,428,203 - -2.2
2,428,385 - cvpA b2313 0.18 -2.3
2,428,458 - cvpA b2313 0.33 -0.9
2,428,510 - cvpA b2313 0.44 -0.1
2,428,649 + cvpA b2313 0.72 -1.8
2,428,657 - cvpA b2313 0.74 +0.1
2,428,657 - cvpA b2313 0.74 -0.9
2,428,684 - cvpA b2313 0.79 -1.0
2,428,723 + cvpA b2313 0.87 -2.7
2,428,775 + -0.1
2,428,775 + -0.0
2,428,816 - -1.9
2,428,818 - +0.1
2,428,818 - -0.4
2,428,986 - -0.4
2,429,015 - +1.3
2,429,016 + -0.1
2,429,072 - -0.9
2,429,111 - dedD b2314 0.10 +0.1
2,429,281 - dedD b2314 0.36 +0.4
2,429,281 - dedD b2314 0.36 +0.9
2,429,287 - dedD b2314 0.37 +0.1
2,429,461 - dedD b2314 0.63 +0.2
2,429,480 + dedD b2314 0.66 +0.5
2,429,596 - dedD b2314 0.83 +0.8
2,429,596 - dedD b2314 0.83 +1.0
2,429,646 - +0.2
2,429,646 - +0.0

Or see this region's nucleotide sequence