Experiment: D-Galacturonic Acid (C) MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yegW and yegX are separated by 51 nucleotides yegX and thiD are separated by 64 nucleotides thiD and thiM overlap by 4 nucleotides
b2101: yegW - predicted DNA-binding transcriptional regulator (NCBI), at 2,180,057 to 2,180,803
yegW
b2102: yegX - orf, hypothetical protein (VIMSS), at 2,180,855 to 2,181,673
yegX
b2103: thiD - phosphomethylpyrimidine kinase (NCBI), at 2,181,738 to 2,182,538
thiD
b2104: thiM - hydroxyethylthiazole kinase (NCBI), at 2,182,535 to 2,183,323
thiM
Position (kb)
2181
2182
2183 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2180.829 kb on + strand at 2180.837 kb on - strand at 2180.886 kb on + strand at 2180.896 kb on - strand at 2180.902 kb on - strand at 2180.902 kb on - strand at 2180.904 kb on - strand at 2180.917 kb on - strand at 2180.934 kb on + strand at 2180.934 kb on + strand at 2180.961 kb on - strand, within yegX at 2181.005 kb on - strand at 2181.005 kb on - strand, within yegX at 2181.027 kb on + strand, within yegX at 2181.027 kb on + strand, within yegX at 2181.202 kb on - strand, within yegX at 2181.202 kb on - strand, within yegX at 2181.221 kb on + strand, within yegX at 2181.256 kb on - strand, within yegX at 2181.376 kb on + strand, within yegX at 2181.460 kb on + strand, within yegX at 2181.467 kb on + strand, within yegX at 2181.485 kb on - strand, within yegX at 2181.497 kb on - strand, within yegX at 2181.516 kb on - strand, within yegX at 2181.525 kb on + strand, within yegX at 2181.548 kb on + strand, within yegX at 2181.628 kb on + strand at 2181.628 kb on + strand at 2181.631 kb on + strand at 2181.660 kb on + strand at 2181.678 kb on - strand at 2181.695 kb on + strand at 2181.726 kb on + strand at 2181.731 kb on - strand at 2181.749 kb on - strand at 2181.801 kb on - strand at 2181.806 kb on - strand at 2181.875 kb on + strand, within thiD at 2181.883 kb on - strand, within thiD at 2181.883 kb on - strand, within thiD at 2181.883 kb on - strand, within thiD at 2182.009 kb on + strand, within thiD at 2182.009 kb on + strand, within thiD at 2182.012 kb on + strand, within thiD at 2182.020 kb on - strand, within thiD at 2182.119 kb on - strand, within thiD at 2182.150 kb on - strand, within thiD at 2182.161 kb on + strand, within thiD at 2182.375 kb on + strand, within thiD at 2182.375 kb on + strand, within thiD at 2182.382 kb on + strand, within thiD at 2182.546 kb on - strand at 2182.646 kb on + strand, within thiM at 2183.006 kb on - strand, within thiM at 2183.006 kb on - strand, within thiM at 2183.044 kb on + strand, within thiM at 2183.246 kb on + strand at 2183.246 kb on + strand at 2183.259 kb on - strand at 2183.259 kb on - strand at 2183.389 kb on - strand at 2183.399 kb on - strand at 2183.399 kb on - strand at 2183.399 kb on - strand at 2183.495 kb on - strand at 2183.533 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galacturonic Acid (C) MOPS remove 2,180,829 + +0.2 2,180,837 - +0.2 2,180,886 + -1.2 2,180,896 - +1.1 2,180,902 - -1.4 2,180,902 - -0.0 2,180,904 - -0.4 2,180,917 - -0.4 2,180,934 + +0.6 2,180,934 + -0.4 2,180,961 - yegX b2102 0.13 -1.4 2,181,005 - -0.0 2,181,005 - yegX b2102 0.18 -1.0 2,181,027 + yegX b2102 0.21 +1.4 2,181,027 + yegX b2102 0.21 -0.7 2,181,202 - yegX b2102 0.42 +0.8 2,181,202 - yegX b2102 0.42 +0.6 2,181,221 + yegX b2102 0.45 -0.4 2,181,256 - yegX b2102 0.49 -0.1 2,181,376 + yegX b2102 0.64 -0.8 2,181,460 + yegX b2102 0.74 -0.4 2,181,467 + yegX b2102 0.75 +0.6 2,181,485 - yegX b2102 0.77 -0.2 2,181,497 - yegX b2102 0.78 -0.5 2,181,516 - yegX b2102 0.81 +0.9 2,181,525 + yegX b2102 0.82 +0.7 2,181,548 + yegX b2102 0.85 +1.2 2,181,628 + +0.6 2,181,628 + +1.2 2,181,631 + -0.2 2,181,660 + +0.0 2,181,678 - +0.4 2,181,695 + -0.0 2,181,726 + +1.0 2,181,731 - +0.4 2,181,749 - -4.0 2,181,801 - -2.6 2,181,806 - -3.6 2,181,875 + thiD b2103 0.17 -2.5 2,181,883 - thiD b2103 0.18 -2.0 2,181,883 - thiD b2103 0.18 -2.6 2,181,883 - thiD b2103 0.18 -2.4 2,182,009 + thiD b2103 0.34 -2.2 2,182,009 + thiD b2103 0.34 -1.8 2,182,012 + thiD b2103 0.34 -3.5 2,182,020 - thiD b2103 0.35 -1.2 2,182,119 - thiD b2103 0.48 -1.3 2,182,150 - thiD b2103 0.51 -2.4 2,182,161 + thiD b2103 0.53 -2.8 2,182,375 + thiD b2103 0.80 -2.2 2,182,375 + thiD b2103 0.80 -1.0 2,182,382 + thiD b2103 0.80 -3.7 2,182,546 - -0.1 2,182,646 + thiM b2104 0.14 -1.5 2,183,006 - thiM b2104 0.60 -0.1 2,183,006 - thiM b2104 0.60 -0.8 2,183,044 + thiM b2104 0.65 -1.4 2,183,246 + -1.8 2,183,246 + -0.7 2,183,259 - +2.2 2,183,259 - -0.7 2,183,389 - -1.0 2,183,399 - +0.6 2,183,399 - -0.8 2,183,399 - +0.6 2,183,495 - -0.4 2,183,533 - -0.2
Or see this region's nucleotide sequence