Strain Fitness in Escherichia coli BW25113 around b1085

Experiment: D-Galacturonic Acid (C) MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrne and b1085 are separated by 134 nucleotidesb1085 and rluC are separated by 117 nucleotides b1084: rne - fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI), at 1,140,405 to 1,143,590 rne b1085: b1085 - orf, hypothetical protein (VIMSS), at 1,143,725 to 1,144,045 b1085 b1086: rluC - 23S rRNA pseudouridylate synthase (NCBI), at 1,144,163 to 1,145,122 rluC Position (kb) 1143 1144 1145Strain fitness (log2 ratio) -1 0 1 2at 1143.725 kb on - strandat 1143.731 kb on - strandat 1143.821 kb on - strand, within b1085at 1143.969 kb on - strand, within b1085at 1143.969 kb on - strand, within b1085at 1144.032 kb on - strandat 1144.039 kb on + strandat 1144.083 kb on + strandat 1144.109 kb on - strandat 1144.145 kb on - strandat 1144.295 kb on + strand, within rluCat 1144.438 kb on - strand, within rluCat 1144.509 kb on - strand, within rluCat 1144.575 kb on - strand, within rluCat 1144.592 kb on + strand, within rluCat 1144.610 kb on - strand, within rluCat 1144.655 kb on + strand, within rluCat 1144.655 kb on + strand, within rluCat 1144.699 kb on + strand, within rluCat 1144.699 kb on + strand, within rluCat 1144.867 kb on + strand, within rluCat 1144.867 kb on + strand, within rluCat 1144.899 kb on + strand, within rluCat 1144.988 kb on + strand, within rluCat 1144.988 kb on + strand, within rluCat 1144.998 kb on + strand, within rluCat 1144.998 kb on + strand, within rluCat 1145.011 kb on - strand, within rluCat 1145.011 kb on - strand, within rluCat 1145.035 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Galacturonic Acid (C) MOPS
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1,143,725 - +1.5
1,143,731 - +2.4
1,143,821 - b1085 b1085 0.30 +1.6
1,143,969 - b1085 b1085 0.76 +0.3
1,143,969 - b1085 b1085 0.76 +0.9
1,144,032 - +1.2
1,144,039 + +0.2
1,144,083 + +0.1
1,144,109 - +1.4
1,144,145 - +0.7
1,144,295 + rluC b1086 0.14 +0.1
1,144,438 - rluC b1086 0.29 -0.2
1,144,509 - rluC b1086 0.36 -0.1
1,144,575 - rluC b1086 0.43 -0.0
1,144,592 + rluC b1086 0.45 -0.0
1,144,610 - rluC b1086 0.47 +1.1
1,144,655 + rluC b1086 0.51 +0.8
1,144,655 + rluC b1086 0.51 +0.5
1,144,699 + rluC b1086 0.56 -0.1
1,144,699 + rluC b1086 0.56 -0.8
1,144,867 + rluC b1086 0.73 -0.1
1,144,867 + rluC b1086 0.73 +1.5
1,144,899 + rluC b1086 0.77 -1.1
1,144,988 + rluC b1086 0.86 +0.1
1,144,988 + rluC b1086 0.86 +1.2
1,144,998 + rluC b1086 0.87 -0.1
1,144,998 + rluC b1086 0.87 -1.5
1,145,011 - rluC b1086 0.88 -0.5
1,145,011 - rluC b1086 0.88 +2.1
1,145,035 + +0.5

Or see this region's nucleotide sequence