Strain Fitness in Escherichia coli BW25113 around b0097

Experiment: D-Galacturonic Acid (C) MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlpxC and yacA are separated by 230 nucleotidesyacA and secA are separated by 61 nucleotides b0096: lpxC - UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (NCBI), at 106,557 to 107,474 lpxC b0097: yacA - orf, hypothetical protein (VIMSS), at 107,705 to 108,217 yacA b0098: secA - translocase (NCBI), at 108,279 to 110,984 secA Position (kb) 107 108 109Strain fitness (log2 ratio) -1 0 1 2at 107.420 kb on + strandat 107.425 kb on + strandat 107.435 kb on + strandat 107.458 kb on + strandat 107.461 kb on - strandat 107.471 kb on - strandat 107.565 kb on + strandat 107.565 kb on + strandat 107.635 kb on + strandat 107.640 kb on + strandat 107.772 kb on - strand, within yacAat 107.822 kb on + strand, within yacAat 107.951 kb on + strand, within yacAat 108.083 kb on + strand, within yacAat 108.083 kb on + strand, within yacAat 108.192 kb on + strandat 108.192 kb on + strandat 108.198 kb on + strandat 108.198 kb on + strandat 108.213 kb on + strandat 108.213 kb on + strandat 108.221 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Galacturonic Acid (C) MOPS
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107,420 + +1.9
107,425 + -0.5
107,435 + +0.3
107,458 + +0.9
107,461 - +0.2
107,471 - -1.7
107,565 + -1.0
107,565 + -0.6
107,635 + +0.3
107,640 + +0.8
107,772 - yacA b0097 0.13 +1.3
107,822 + yacA b0097 0.23 +1.3
107,951 + yacA b0097 0.48 +0.9
108,083 + yacA b0097 0.74 -0.0
108,083 + yacA b0097 0.74 -0.1
108,192 + -0.9
108,192 + -0.1
108,198 + -1.1
108,198 + +0.4
108,213 + +1.5
108,213 + -0.5
108,221 + +0.8

Or see this region's nucleotide sequence