Experiment: D-Galacturonic Acid (C) MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rluA and hepA are separated by 11 nucleotides hepA and polB are separated by 164 nucleotides
b0058: rluA - pseudouridine synthase for 23S rRNA (position 746) and tRNAphe(position 32) (NCBI), at 59,687 to 60,346
rluA
b0059: hepA - ATP-dependent helicase HepA (NCBI), at 60,358 to 63,264
hepA
b0060: polB - DNA polymerase II (NCBI), at 63,429 to 65,780
polB
Position (kb) 60
61
62
63
64 Strain fitness (log2 ratio) -2
-1
0
1
2 at 59.365 kb on - strand at 59.375 kb on - strand at 59.376 kb on - strand at 59.376 kb on - strand at 59.377 kb on + strand at 59.378 kb on - strand at 59.378 kb on - strand at 59.380 kb on + strand at 59.383 kb on + strand at 59.386 kb on - strand at 59.388 kb on - strand at 59.395 kb on + strand at 59.395 kb on + strand at 59.395 kb on + strand at 59.395 kb on + strand at 59.395 kb on + strand at 59.398 kb on - strand at 59.400 kb on + strand at 59.400 kb on + strand at 59.400 kb on + strand at 59.400 kb on - strand at 59.400 kb on - strand at 59.403 kb on - strand at 59.403 kb on - strand at 59.407 kb on + strand at 59.408 kb on - strand at 59.408 kb on - strand at 59.408 kb on - strand at 59.408 kb on - strand at 59.411 kb on + strand at 59.414 kb on + strand at 59.414 kb on + strand at 59.419 kb on - strand at 59.422 kb on - strand at 59.424 kb on - strand at 59.432 kb on - strand at 59.446 kb on + strand at 59.446 kb on - strand at 59.450 kb on + strand at 59.452 kb on + strand at 59.469 kb on + strand at 59.469 kb on + strand at 59.477 kb on - strand at 59.477 kb on - strand at 59.477 kb on - strand at 59.477 kb on - strand at 59.484 kb on + strand at 59.503 kb on - strand at 59.505 kb on + strand at 59.557 kb on - strand at 59.640 kb on + strand at 59.648 kb on - strand at 59.653 kb on + strand at 59.655 kb on + strand at 59.660 kb on - strand at 59.660 kb on - strand at 59.661 kb on - strand at 59.661 kb on - strand at 59.661 kb on - strand at 59.668 kb on - strand at 59.685 kb on + strand at 59.767 kb on - strand, within rluA at 59.841 kb on + strand, within rluA at 59.855 kb on - strand, within rluA at 59.880 kb on + strand, within rluA at 59.895 kb on - strand, within rluA at 59.909 kb on - strand, within rluA at 59.910 kb on + strand, within rluA at 59.926 kb on - strand, within rluA at 59.935 kb on - strand, within rluA at 59.935 kb on - strand, within rluA at 59.941 kb on + strand, within rluA at 59.968 kb on + strand, within rluA at 59.968 kb on + strand, within rluA at 60.042 kb on + strand, within rluA at 60.143 kb on - strand, within rluA at 60.225 kb on - strand, within rluA at 60.236 kb on + strand, within rluA at 60.264 kb on + strand, within rluA at 60.274 kb on + strand, within rluA at 60.282 kb on - strand at 60.282 kb on - strand at 60.304 kb on - strand at 60.304 kb on - strand at 60.363 kb on - strand at 60.363 kb on - strand at 60.389 kb on + strand at 60.389 kb on + strand at 60.411 kb on + strand at 60.411 kb on + strand at 60.419 kb on - strand at 60.440 kb on - strand at 60.452 kb on - strand at 60.490 kb on + strand at 60.490 kb on + strand at 60.551 kb on + strand at 60.580 kb on - strand at 60.686 kb on - strand, within hepA at 60.686 kb on - strand, within hepA at 60.738 kb on + strand, within hepA at 60.738 kb on + strand, within hepA at 60.774 kb on + strand, within hepA at 60.897 kb on + strand, within hepA at 60.898 kb on + strand, within hepA at 60.940 kb on - strand, within hepA at 61.005 kb on + strand, within hepA at 61.005 kb on + strand, within hepA at 61.005 kb on + strand, within hepA at 61.013 kb on - strand, within hepA at 61.026 kb on + strand, within hepA at 61.034 kb on - strand, within hepA at 61.049 kb on - strand, within hepA at 61.052 kb on + strand, within hepA at 61.052 kb on + strand, within hepA at 61.055 kb on - strand, within hepA at 61.101 kb on + strand, within hepA at 61.102 kb on + strand, within hepA at 61.109 kb on - strand, within hepA at 61.109 kb on - strand, within hepA at 61.130 kb on - strand, within hepA at 61.162 kb on + strand, within hepA at 61.162 kb on + strand, within hepA at 61.228 kb on + strand, within hepA at 61.228 kb on + strand, within hepA at 61.277 kb on - strand, within hepA at 61.374 kb on + strand, within hepA at 61.378 kb on - strand, within hepA at 61.378 kb on - strand, within hepA at 61.378 kb on - strand, within hepA at 61.455 kb on + strand, within hepA at 61.469 kb on - strand, within hepA at 61.470 kb on + strand, within hepA at 61.526 kb on - strand, within hepA at 61.538 kb on - strand, within hepA at 61.550 kb on + strand, within hepA at 61.582 kb on + strand, within hepA at 61.584 kb on + strand, within hepA at 61.585 kb on + strand, within hepA at 61.585 kb on + strand, within hepA at 61.591 kb on - strand, within hepA at 61.591 kb on - strand, within hepA at 61.658 kb on - strand, within hepA at 61.658 kb on - strand, within hepA at 61.675 kb on + strand, within hepA at 61.693 kb on + strand, within hepA at 61.713 kb on + strand, within hepA at 61.781 kb on - strand, within hepA at 61.856 kb on + strand, within hepA at 61.856 kb on + strand, within hepA at 61.859 kb on - strand, within hepA at 61.864 kb on - strand, within hepA at 61.953 kb on + strand, within hepA at 61.994 kb on - strand, within hepA at 62.011 kb on + strand, within hepA at 62.129 kb on + strand, within hepA at 62.147 kb on + strand, within hepA at 62.191 kb on - strand, within hepA at 62.191 kb on - strand, within hepA at 62.303 kb on + strand, within hepA at 62.303 kb on + strand, within hepA at 62.321 kb on - strand, within hepA at 62.324 kb on + strand, within hepA at 62.370 kb on - strand, within hepA at 62.370 kb on - strand, within hepA at 62.406 kb on + strand, within hepA at 62.406 kb on + strand, within hepA at 62.440 kb on + strand, within hepA at 62.541 kb on - strand, within hepA at 62.541 kb on - strand, within hepA at 62.545 kb on + strand, within hepA at 62.558 kb on + strand, within hepA at 62.558 kb on + strand, within hepA at 62.558 kb on + strand, within hepA at 62.566 kb on - strand, within hepA at 62.570 kb on + strand, within hepA at 62.578 kb on - strand, within hepA at 62.625 kb on + strand, within hepA at 62.625 kb on + strand, within hepA at 62.626 kb on - strand, within hepA at 62.631 kb on - strand, within hepA at 62.694 kb on + strand, within hepA at 62.810 kb on + strand, within hepA at 62.810 kb on + strand, within hepA at 62.810 kb on + strand, within hepA at 62.813 kb on - strand, within hepA at 62.926 kb on + strand, within hepA at 62.934 kb on - strand, within hepA at 62.976 kb on + strand at 62.976 kb on + strand at 62.981 kb on + strand at 63.103 kb on + strand at 63.260 kb on - strand at 63.318 kb on + strand at 63.324 kb on - strand at 63.451 kb on + strand at 63.524 kb on + strand at 63.528 kb on - strand at 63.528 kb on - strand at 63.532 kb on + strand at 63.545 kb on - strand at 63.577 kb on + strand at 63.577 kb on + strand at 63.627 kb on + strand at 63.683 kb on + strand, within polB at 63.727 kb on + strand, within polB at 63.821 kb on + strand, within polB at 63.821 kb on + strand, within polB at 63.830 kb on - strand, within polB at 63.920 kb on - strand, within polB at 63.948 kb on + strand, within polB at 63.994 kb on + strand, within polB at 63.994 kb on + strand, within polB at 64.007 kb on + strand, within polB at 64.120 kb on - strand, within polB
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galacturonic Acid (C) MOPS remove 59,365 - -0.3 59,375 - -0.7 59,376 - +0.9 59,376 - +0.9 59,377 + -0.0 59,378 - +0.2 59,378 - +0.9 59,380 + -0.5 59,383 + -0.9 59,386 - -0.5 59,388 - -1.9 59,395 + +0.4 59,395 + +0.9 59,395 + -1.0 59,395 + +0.2 59,395 + +0.1 59,398 - -0.2 59,400 + -1.2 59,400 + -0.1 59,400 + -0.3 59,400 - +1.1 59,400 - -0.1 59,403 - +1.8 59,403 - +0.8 59,407 + -0.1 59,408 - +0.3 59,408 - -1.1 59,408 - -0.1 59,408 - +1.4 59,411 + -0.4 59,414 + +0.3 59,414 + +0.9 59,419 - +0.1 59,422 - -1.9 59,424 - -0.0 59,432 - +0.3 59,446 + +0.1 59,446 - +0.9 59,450 + -0.2 59,452 + -0.6 59,469 + -0.1 59,469 + +1.2 59,477 - -0.7 59,477 - +0.0 59,477 - +0.3 59,477 - +1.0 59,484 + -0.1 59,503 - +0.8 59,505 + +2.5 59,557 - -0.6 59,640 + -0.8 59,648 - -0.3 59,653 + -0.6 59,655 + -0.4 59,660 - -0.1 59,660 - +0.6 59,661 - +0.4 59,661 - -0.4 59,661 - -0.7 59,668 - +1.4 59,685 + +0.9 59,767 - rluA b0058 0.12 -0.3 59,841 + rluA b0058 0.23 +0.8 59,855 - rluA b0058 0.25 -0.2 59,880 + rluA b0058 0.29 -1.0 59,895 - rluA b0058 0.32 -0.1 59,909 - rluA b0058 0.34 -0.5 59,910 + rluA b0058 0.34 -0.1 59,926 - rluA b0058 0.36 -0.2 59,935 - rluA b0058 0.38 -0.1 59,935 - rluA b0058 0.38 -0.5 59,941 + rluA b0058 0.38 +1.0 59,968 + rluA b0058 0.43 -0.7 59,968 + rluA b0058 0.43 -1.1 60,042 + rluA b0058 0.54 +1.0 60,143 - rluA b0058 0.69 +0.5 60,225 - rluA b0058 0.82 +0.5 60,236 + rluA b0058 0.83 +0.1 60,264 + rluA b0058 0.87 +0.2 60,274 + rluA b0058 0.89 +0.0 60,282 - +0.9 60,282 - -0.1 60,304 - -1.4 60,304 - -0.5 60,363 - +0.5 60,363 - -0.5 60,389 + +1.4 60,389 + +1.4 60,411 + -0.2 60,411 + +1.4 60,419 - -0.2 60,440 - +0.6 60,452 - +1.4 60,490 + -0.0 60,490 + +0.4 60,551 + +0.7 60,580 - -0.1 60,686 - hepA b0059 0.11 +0.3 60,686 - hepA b0059 0.11 +1.5 60,738 + hepA b0059 0.13 +0.3 60,738 + hepA b0059 0.13 +0.7 60,774 + hepA b0059 0.14 +0.9 60,897 + hepA b0059 0.19 -0.4 60,898 + hepA b0059 0.19 +1.0 60,940 - hepA b0059 0.20 +0.2 61,005 + hepA b0059 0.22 -0.0 61,005 + hepA b0059 0.22 +0.3 61,005 + hepA b0059 0.22 +0.6 61,013 - hepA b0059 0.23 +0.9 61,026 + hepA b0059 0.23 +0.8 61,034 - hepA b0059 0.23 +0.2 61,049 - hepA b0059 0.24 +0.7 61,052 + hepA b0059 0.24 +0.7 61,052 + hepA b0059 0.24 +0.7 61,055 - hepA b0059 0.24 +0.2 61,101 + hepA b0059 0.26 +0.4 61,102 + hepA b0059 0.26 +1.0 61,109 - hepA b0059 0.26 -0.2 61,109 - hepA b0059 0.26 +0.5 61,130 - hepA b0059 0.27 +0.3 61,162 + hepA b0059 0.28 +0.3 61,162 + hepA b0059 0.28 +0.5 61,228 + hepA b0059 0.30 +0.3 61,228 + hepA b0059 0.30 +1.3 61,277 - hepA b0059 0.32 -0.5 61,374 + hepA b0059 0.35 -0.4 61,378 - hepA b0059 0.35 +1.3 61,378 - hepA b0059 0.35 +0.4 61,378 - hepA b0059 0.35 +0.5 61,455 + hepA b0059 0.38 -0.2 61,469 - hepA b0059 0.38 +0.6 61,470 + hepA b0059 0.38 +0.0 61,526 - hepA b0059 0.40 +1.1 61,538 - hepA b0059 0.41 +0.6 61,550 + hepA b0059 0.41 -0.1 61,582 + hepA b0059 0.42 -0.0 61,584 + hepA b0059 0.42 +0.8 61,585 + hepA b0059 0.42 -0.8 61,585 + hepA b0059 0.42 +1.9 61,591 - hepA b0059 0.42 +1.1 61,591 - hepA b0059 0.42 -0.4 61,658 - hepA b0059 0.45 -2.2 61,658 - hepA b0059 0.45 -0.1 61,675 + hepA b0059 0.45 +1.1 61,693 + hepA b0059 0.46 +0.4 61,713 + hepA b0059 0.47 +0.9 61,781 - hepA b0059 0.49 -0.5 61,856 + hepA b0059 0.52 -0.1 61,856 + hepA b0059 0.52 +0.9 61,859 - hepA b0059 0.52 +0.9 61,864 - hepA b0059 0.52 -0.9 61,953 + hepA b0059 0.55 -0.2 61,994 - hepA b0059 0.56 +1.9 62,011 + hepA b0059 0.57 +0.7 62,129 + hepA b0059 0.61 -0.1 62,147 + hepA b0059 0.62 +0.2 62,191 - hepA b0059 0.63 +0.3 62,191 - hepA b0059 0.63 +2.0 62,303 + hepA b0059 0.67 +0.8 62,303 + hepA b0059 0.67 +1.1 62,321 - hepA b0059 0.68 +0.6 62,324 + hepA b0059 0.68 -1.2 62,370 - hepA b0059 0.69 +2.0 62,370 - hepA b0059 0.69 +0.9 62,406 + hepA b0059 0.70 +0.9 62,406 + hepA b0059 0.70 +0.7 62,440 + hepA b0059 0.72 +0.7 62,541 - hepA b0059 0.75 +0.0 62,541 - hepA b0059 0.75 +1.1 62,545 + hepA b0059 0.75 +0.3 62,558 + hepA b0059 0.76 +1.4 62,558 + hepA b0059 0.76 +0.9 62,558 + hepA b0059 0.76 +0.3 62,566 - hepA b0059 0.76 -0.0 62,570 + hepA b0059 0.76 +1.8 62,578 - hepA b0059 0.76 +1.9 62,625 + hepA b0059 0.78 +0.1 62,625 + hepA b0059 0.78 +0.1 62,626 - hepA b0059 0.78 +0.5 62,631 - hepA b0059 0.78 +1.0 62,694 + hepA b0059 0.80 +0.4 62,810 + hepA b0059 0.84 -0.4 62,810 + hepA b0059 0.84 +0.9 62,810 + hepA b0059 0.84 +0.6 62,813 - hepA b0059 0.84 +0.3 62,926 + hepA b0059 0.88 +0.8 62,934 - hepA b0059 0.89 +0.7 62,976 + +0.5 62,976 + +0.9 62,981 + -0.2 63,103 + +0.1 63,260 - +1.1 63,318 + -0.3 63,324 - -0.7 63,451 + -0.1 63,524 + -1.3 63,528 - -0.1 63,528 - +0.5 63,532 + +1.2 63,545 - +0.6 63,577 + +0.5 63,577 + +0.3 63,627 + +1.3 63,683 + polB b0060 0.11 -0.5 63,727 + polB b0060 0.13 +0.1 63,821 + polB b0060 0.17 +2.2 63,821 + polB b0060 0.17 +0.9 63,830 - polB b0060 0.17 +0.9 63,920 - polB b0060 0.21 +0.9 63,948 + polB b0060 0.22 +0.8 63,994 + polB b0060 0.24 +0.9 63,994 + polB b0060 0.24 -0.1 64,007 + polB b0060 0.25 -0.0 64,120 - polB b0060 0.29 +0.3
Or see this region's nucleotide sequence