Experiment: D-Galacturonic Acid (C) MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insL-1 and mokC are separated by 193 nucleotides mokC and hokC overlap by 210 nucleotides hokC and sokC are separated by 48 nucleotides sokC and nhaA are separated by 482 nucleotides
b0016: insL-1 - IS186/IS421 transposase (NCBI), at 15,445 to 16,557
insL-1
b0018: mokC - regulatory protein for HokC, overlaps CDS of hokC (NCBI), at 16,751 to 16,960
mokC
b4412: hokC - toxic membrane protein, small (NCBI), at 16,751 to 16,903
hokC
b4413: sokC - regulatory antisense RNA (NCBI), at 16,952 to 17,006
sokC
b0019: nhaA - pH-dependent sodium/proton antiporter (NCBI), at 17,489 to 18,655
nhaA
Position (kb)
16
17 Strain fitness (log2 ratio)
-1
0
1
2 at 16.703 kb on + strand at 16.729 kb on + strand at 16.729 kb on + strand at 16.895 kb on - strand at 16.895 kb on - strand at 16.920 kb on + strand, within mokC at 16.942 kb on + strand at 16.950 kb on - strand at 16.950 kb on - strand at 16.950 kb on - strand at 16.950 kb on - strand at 16.950 kb on - strand at 16.950 kb on - strand at 16.955 kb on - strand at 16.957 kb on + strand at 16.957 kb on + strand at 16.957 kb on + strand at 16.976 kb on - strand, within sokC at 17.024 kb on - strand at 17.032 kb on + strand at 17.037 kb on + strand at 17.037 kb on + strand at 17.058 kb on + strand at 17.058 kb on + strand at 17.058 kb on + strand at 17.060 kb on + strand at 17.060 kb on + strand at 17.096 kb on + strand at 17.097 kb on + strand at 17.104 kb on + strand at 17.104 kb on + strand at 17.112 kb on - strand at 17.306 kb on + strand at 17.318 kb on - strand at 17.330 kb on - strand at 17.339 kb on - strand at 17.339 kb on - strand at 17.503 kb on - strand at 17.517 kb on - strand at 17.517 kb on - strand at 17.517 kb on - strand at 17.537 kb on + strand at 17.555 kb on + strand at 17.581 kb on - strand at 17.600 kb on + strand at 17.621 kb on + strand, within nhaA at 17.671 kb on + strand, within nhaA at 17.676 kb on - strand at 17.749 kb on - strand, within nhaA at 17.749 kb on - strand, within nhaA at 17.765 kb on - strand, within nhaA at 17.765 kb on - strand, within nhaA at 17.813 kb on + strand, within nhaA at 17.879 kb on + strand, within nhaA at 17.887 kb on - strand, within nhaA at 17.902 kb on - strand, within nhaA at 17.904 kb on + strand, within nhaA at 17.917 kb on + strand, within nhaA at 17.925 kb on - strand, within nhaA at 17.925 kb on - strand, within nhaA at 17.925 kb on - strand, within nhaA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galacturonic Acid (C) MOPS remove 16,703 + -0.0 16,729 + -1.1 16,729 + -1.7 16,895 - -0.1 16,895 - -0.5 16,920 + mokC b0018 0.80 +1.5 16,942 + -1.7 16,950 - +0.5 16,950 - -0.1 16,950 - -1.5 16,950 - +0.3 16,950 - +0.4 16,950 - +0.7 16,955 - -0.1 16,957 + +0.1 16,957 + -0.5 16,957 + -0.0 16,976 - sokC b4413 0.44 +0.7 17,024 - -0.0 17,032 + -0.4 17,037 + -1.2 17,037 + +0.0 17,058 + +1.4 17,058 + -0.8 17,058 + -0.1 17,060 + -1.1 17,060 + -0.5 17,096 + +0.2 17,097 + +0.1 17,104 + +0.5 17,104 + +0.4 17,112 - -0.2 17,306 + +0.3 17,318 - -0.5 17,330 - -0.3 17,339 - -0.1 17,339 - +0.0 17,503 - +0.0 17,517 - -0.3 17,517 - -0.1 17,517 - +1.3 17,537 + -1.0 17,555 + -0.4 17,581 - +0.9 17,600 + +0.4 17,621 + nhaA b0019 0.11 -0.0 17,671 + nhaA b0019 0.16 +0.9 17,676 - -0.1 17,749 - nhaA b0019 0.22 +0.6 17,749 - nhaA b0019 0.22 -1.6 17,765 - nhaA b0019 0.24 -0.9 17,765 - nhaA b0019 0.24 +2.2 17,813 + nhaA b0019 0.28 +1.1 17,879 + nhaA b0019 0.33 -0.1 17,887 - nhaA b0019 0.34 +0.9 17,902 - nhaA b0019 0.35 -0.8 17,904 + nhaA b0019 0.36 -0.1 17,917 + nhaA b0019 0.37 -1.1 17,925 - nhaA b0019 0.37 -0.8 17,925 - nhaA b0019 0.37 -0.1 17,925 - nhaA b0019 0.37 +0.3
Or see this region's nucleotide sequence