Experiment: D-Galacturonic Acid (C) MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrA and thrB are separated by 1 nucleotides thrB and thrC are separated by 0 nucleotides
b0002: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI), at 337 to 2,799
thrA
b0003: thrB - homoserine kinase (NCBI), at 2,801 to 3,733
thrB
b0004: thrC - threonine synthase (NCBI), at 3,734 to 5,020
thrC
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1.868 kb on + strand, within thrA at 1.868 kb on + strand, within thrA at 1.871 kb on + strand, within thrA at 1.876 kb on - strand, within thrA at 1.881 kb on - strand, within thrA at 1.881 kb on - strand, within thrA at 1.929 kb on + strand, within thrA at 1.929 kb on + strand, within thrA at 1.937 kb on - strand, within thrA at 1.937 kb on - strand, within thrA at 2.076 kb on + strand, within thrA at 2.104 kb on + strand, within thrA at 2.104 kb on + strand, within thrA at 2.266 kb on - strand, within thrA at 2.266 kb on - strand, within thrA at 2.292 kb on - strand, within thrA at 2.294 kb on + strand, within thrA at 2.370 kb on - strand, within thrA at 2.370 kb on - strand, within thrA at 2.449 kb on - strand, within thrA at 2.590 kb on - strand at 2.675 kb on + strand at 2.682 kb on + strand at 2.682 kb on + strand at 2.684 kb on + strand at 2.717 kb on + strand at 2.744 kb on - strand at 2.787 kb on - strand at 2.787 kb on - strand at 2.829 kb on + strand at 2.847 kb on + strand at 2.847 kb on + strand at 2.906 kb on - strand, within thrB at 2.934 kb on + strand, within thrB at 2.963 kb on - strand, within thrB at 3.020 kb on - strand, within thrB at 3.023 kb on + strand, within thrB at 3.131 kb on + strand, within thrB at 3.144 kb on - strand, within thrB at 3.186 kb on + strand, within thrB at 3.246 kb on + strand, within thrB at 3.248 kb on + strand, within thrB at 3.256 kb on + strand, within thrB at 3.293 kb on + strand, within thrB at 3.301 kb on - strand, within thrB at 3.318 kb on - strand, within thrB at 3.411 kb on + strand, within thrB at 3.444 kb on - strand, within thrB at 3.444 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.509 kb on - strand, within thrB at 3.511 kb on + strand, within thrB at 3.607 kb on + strand, within thrB at 3.634 kb on - strand, within thrB at 3.638 kb on + strand, within thrB at 3.638 kb on + strand, within thrB at 3.650 kb on + strand at 3.769 kb on + strand at 3.769 kb on + strand at 3.792 kb on - strand at 3.805 kb on - strand at 3.953 kb on - strand, within thrC at 3.953 kb on - strand, within thrC at 4.108 kb on - strand, within thrC at 4.174 kb on - strand, within thrC at 4.180 kb on + strand, within thrC at 4.180 kb on + strand, within thrC at 4.216 kb on + strand, within thrC at 4.253 kb on - strand, within thrC at 4.253 kb on - strand, within thrC at 4.464 kb on - strand, within thrC at 4.466 kb on + strand, within thrC at 4.619 kb on + strand, within thrC at 4.721 kb on + strand, within thrC at 4.729 kb on + strand, within thrC at 4.729 kb on - strand, within thrC at 4.729 kb on - strand, within thrC
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galacturonic Acid (C) MOPS remove 1,868 + thrA b0002 0.62 -1.9 1,868 + thrA b0002 0.62 -2.8 1,871 + thrA b0002 0.62 -2.6 1,876 - thrA b0002 0.62 -2.7 1,881 - thrA b0002 0.63 -2.3 1,881 - thrA b0002 0.63 -1.7 1,929 + thrA b0002 0.65 -3.4 1,929 + thrA b0002 0.65 -3.5 1,937 - thrA b0002 0.65 +0.9 1,937 - thrA b0002 0.65 +0.9 2,076 + thrA b0002 0.71 -1.7 2,104 + thrA b0002 0.72 -2.4 2,104 + thrA b0002 0.72 -2.6 2,266 - thrA b0002 0.78 -2.8 2,266 - thrA b0002 0.78 -2.1 2,292 - thrA b0002 0.79 -2.6 2,294 + thrA b0002 0.79 -1.7 2,370 - thrA b0002 0.83 -2.7 2,370 - thrA b0002 0.83 -2.1 2,449 - thrA b0002 0.86 -3.2 2,590 - -2.1 2,675 + -1.6 2,682 + -1.1 2,682 + -0.1 2,684 + -1.6 2,717 + -2.4 2,744 - -3.0 2,787 - -3.2 2,787 - -1.4 2,829 + -3.1 2,847 + -2.1 2,847 + -2.3 2,906 - thrB b0003 0.11 -1.7 2,934 + thrB b0003 0.14 -3.9 2,963 - thrB b0003 0.17 -3.0 3,020 - thrB b0003 0.23 -0.5 3,023 + thrB b0003 0.24 -2.4 3,131 + thrB b0003 0.35 -3.7 3,144 - thrB b0003 0.37 -1.4 3,186 + thrB b0003 0.41 -3.3 3,246 + thrB b0003 0.48 -2.1 3,248 + thrB b0003 0.48 -4.1 3,256 + thrB b0003 0.49 -3.7 3,293 + thrB b0003 0.53 -0.1 3,301 - thrB b0003 0.54 -1.9 3,318 - thrB b0003 0.55 -3.5 3,411 + thrB b0003 0.65 -3.2 3,444 - thrB b0003 0.69 -1.7 3,444 - thrB b0003 0.69 -0.1 3,504 - thrB b0003 0.75 -3.9 3,504 - thrB b0003 0.75 -2.1 3,509 - thrB b0003 0.76 -4.4 3,511 + thrB b0003 0.76 -2.1 3,607 + thrB b0003 0.86 -2.8 3,634 - thrB b0003 0.89 -1.9 3,638 + thrB b0003 0.90 -3.9 3,638 + thrB b0003 0.90 -1.7 3,650 + -1.9 3,769 + -1.7 3,769 + -4.2 3,792 - -3.4 3,805 - -1.4 3,953 - thrC b0004 0.17 -1.7 3,953 - thrC b0004 0.17 -3.2 4,108 - thrC b0004 0.29 -3.6 4,174 - thrC b0004 0.34 -2.3 4,180 + thrC b0004 0.35 -2.3 4,180 + thrC b0004 0.35 -2.8 4,216 + thrC b0004 0.37 -2.9 4,253 - thrC b0004 0.40 -1.9 4,253 - thrC b0004 0.40 -2.1 4,464 - thrC b0004 0.57 -1.9 4,466 + thrC b0004 0.57 -0.7 4,619 + thrC b0004 0.69 -3.4 4,721 + thrC b0004 0.77 -2.1 4,729 + thrC b0004 0.77 -2.7 4,729 - thrC b0004 0.77 -3.6 4,729 - thrC b0004 0.77 -1.7
Or see this region's nucleotide sequence