Experiment: L-Lysine 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yrdC and yrdD are separated by 4 nucleotides yrdD and smg are separated by 28 nucleotides smg and smf overlap by 29 nucleotides
b3282: yrdC - predicted ribosome maturation factor (NCBI), at 3,428,865 to 3,429,437
yrdC
b3283: yrdD - predicted DNA topoisomerase (NCBI), at 3,429,442 to 3,429,984
yrdD
b3284: smg - hypothetical protein (NCBI), at 3,430,013 to 3,430,486
smg
b4473: smf - hypothetical protein (NCBI), at 3,430,458 to 3,431,582
smf
Position (kb)
3430
3431 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3429.403 kb on + strand at 3429.447 kb on - strand at 3429.489 kb on + strand at 3429.489 kb on + strand at 3429.489 kb on + strand at 3429.530 kb on + strand, within yrdD at 3429.530 kb on + strand, within yrdD at 3429.532 kb on + strand, within yrdD at 3429.597 kb on + strand, within yrdD at 3429.598 kb on - strand, within yrdD at 3429.598 kb on - strand, within yrdD at 3429.607 kb on - strand, within yrdD at 3429.623 kb on + strand, within yrdD at 3429.634 kb on + strand, within yrdD at 3429.634 kb on + strand, within yrdD at 3429.642 kb on - strand, within yrdD at 3429.642 kb on - strand, within yrdD at 3429.642 kb on - strand, within yrdD at 3429.642 kb on - strand, within yrdD at 3429.642 kb on - strand, within yrdD at 3429.643 kb on + strand, within yrdD at 3429.643 kb on - strand, within yrdD at 3429.651 kb on - strand, within yrdD at 3429.653 kb on + strand, within yrdD at 3429.653 kb on + strand, within yrdD at 3429.726 kb on + strand, within yrdD at 3429.742 kb on + strand, within yrdD at 3429.750 kb on - strand, within yrdD at 3429.750 kb on - strand, within yrdD at 3429.765 kb on - strand, within yrdD at 3429.785 kb on + strand, within yrdD at 3429.786 kb on + strand, within yrdD at 3429.794 kb on - strand, within yrdD at 3429.794 kb on - strand, within yrdD at 3429.805 kb on + strand, within yrdD at 3429.863 kb on + strand, within yrdD at 3429.888 kb on + strand, within yrdD at 3429.891 kb on - strand, within yrdD at 3429.924 kb on - strand, within yrdD at 3429.933 kb on + strand at 3429.946 kb on - strand at 3429.956 kb on - strand at 3429.956 kb on - strand at 3429.958 kb on - strand at 3429.959 kb on + strand at 3429.959 kb on + strand at 3429.960 kb on + strand at 3429.960 kb on + strand at 3429.967 kb on - strand at 3429.970 kb on - strand at 3429.972 kb on - strand at 3429.972 kb on - strand at 3429.977 kb on - strand at 3429.996 kb on - strand at 3429.996 kb on - strand at 3430.016 kb on + strand at 3430.021 kb on + strand at 3430.024 kb on - strand at 3430.025 kb on + strand at 3430.084 kb on + strand, within smg at 3430.086 kb on + strand, within smg at 3430.113 kb on - strand, within smg at 3430.136 kb on - strand, within smg at 3430.136 kb on - strand, within smg at 3430.137 kb on - strand, within smg at 3430.148 kb on - strand, within smg at 3430.205 kb on - strand, within smg at 3430.247 kb on - strand, within smg at 3430.249 kb on + strand, within smg at 3430.249 kb on + strand, within smg at 3430.266 kb on - strand, within smg at 3430.272 kb on - strand, within smg at 3430.283 kb on - strand, within smg at 3430.289 kb on + strand, within smg at 3430.362 kb on + strand, within smg at 3430.362 kb on + strand, within smg at 3430.372 kb on - strand, within smg at 3430.415 kb on + strand, within smg at 3430.423 kb on - strand, within smg at 3430.432 kb on + strand, within smg at 3430.432 kb on + strand, within smg at 3430.432 kb on + strand, within smg at 3430.481 kb on + strand at 3430.490 kb on - strand at 3430.491 kb on + strand at 3430.512 kb on + strand at 3430.513 kb on + strand at 3430.515 kb on + strand at 3430.518 kb on + strand at 3430.518 kb on + strand at 3430.523 kb on - strand at 3430.523 kb on - strand at 3430.525 kb on + strand at 3430.531 kb on - strand at 3430.548 kb on - strand at 3430.568 kb on - strand at 3430.568 kb on - strand at 3430.570 kb on - strand at 3430.583 kb on - strand, within smf at 3430.590 kb on - strand, within smf at 3430.595 kb on + strand, within smf at 3430.595 kb on + strand, within smf at 3430.602 kb on - strand, within smf at 3430.616 kb on + strand, within smf at 3430.616 kb on + strand, within smf at 3430.616 kb on - strand, within smf at 3430.637 kb on - strand, within smf at 3430.671 kb on + strand, within smf at 3430.793 kb on + strand, within smf at 3430.801 kb on - strand, within smf at 3430.815 kb on - strand, within smf at 3430.828 kb on - strand, within smf at 3430.828 kb on - strand, within smf at 3430.871 kb on + strand, within smf at 3430.876 kb on + strand, within smf at 3430.876 kb on - strand, within smf at 3430.881 kb on - strand, within smf at 3430.891 kb on + strand, within smf at 3430.891 kb on + strand, within smf at 3430.921 kb on + strand, within smf at 3430.921 kb on + strand, within smf at 3430.927 kb on - strand, within smf at 3430.944 kb on - strand, within smf at 3430.944 kb on - strand, within smf at 3430.980 kb on - strand, within smf at 3430.984 kb on - strand, within smf at 3431.013 kb on + strand, within smf at 3431.017 kb on + strand, within smf at 3431.018 kb on + strand, within smf at 3431.065 kb on + strand, within smf at 3431.073 kb on - strand, within smf at 3431.079 kb on + strand, within smf at 3431.126 kb on + strand, within smf at 3431.134 kb on - strand, within smf at 3431.134 kb on - strand, within smf at 3431.221 kb on + strand, within smf at 3431.373 kb on + strand, within smf at 3431.376 kb on - strand, within smf at 3431.415 kb on - strand, within smf at 3431.415 kb on - strand, within smf at 3431.448 kb on + strand, within smf at 3431.448 kb on + strand, within smf at 3431.455 kb on - strand, within smf at 3431.455 kb on - strand, within smf at 3431.468 kb on + strand, within smf at 3431.474 kb on - strand at 3431.482 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 200 mM remove 3,429,403 + +0.4 3,429,447 - +0.2 3,429,489 + +1.0 3,429,489 + +0.1 3,429,489 + +1.2 3,429,530 + yrdD b3283 0.16 -0.4 3,429,530 + yrdD b3283 0.16 +0.6 3,429,532 + yrdD b3283 0.17 +1.4 3,429,597 + yrdD b3283 0.29 +0.1 3,429,598 - yrdD b3283 0.29 +0.0 3,429,598 - yrdD b3283 0.29 -0.5 3,429,607 - yrdD b3283 0.30 +0.8 3,429,623 + yrdD b3283 0.33 -0.6 3,429,634 + yrdD b3283 0.35 +0.0 3,429,634 + yrdD b3283 0.35 +0.3 3,429,642 - yrdD b3283 0.37 +0.1 3,429,642 - yrdD b3283 0.37 +0.8 3,429,642 - yrdD b3283 0.37 -0.0 3,429,642 - yrdD b3283 0.37 +0.6 3,429,642 - yrdD b3283 0.37 -1.3 3,429,643 + yrdD b3283 0.37 +0.8 3,429,643 - yrdD b3283 0.37 -0.5 3,429,651 - yrdD b3283 0.38 -0.8 3,429,653 + yrdD b3283 0.39 -0.5 3,429,653 + yrdD b3283 0.39 -0.0 3,429,726 + yrdD b3283 0.52 -0.3 3,429,742 + yrdD b3283 0.55 +1.0 3,429,750 - yrdD b3283 0.57 +0.2 3,429,750 - yrdD b3283 0.57 +0.9 3,429,765 - yrdD b3283 0.59 -0.6 3,429,785 + yrdD b3283 0.63 +0.4 3,429,786 + yrdD b3283 0.63 -0.8 3,429,794 - yrdD b3283 0.65 +1.4 3,429,794 - yrdD b3283 0.65 -0.5 3,429,805 + yrdD b3283 0.67 +0.2 3,429,863 + yrdD b3283 0.78 +0.2 3,429,888 + yrdD b3283 0.82 +0.4 3,429,891 - yrdD b3283 0.83 -0.9 3,429,924 - yrdD b3283 0.89 -0.2 3,429,933 + +0.8 3,429,946 - +0.1 3,429,956 - +0.5 3,429,956 - +0.7 3,429,958 - -0.2 3,429,959 + +0.4 3,429,959 + -0.5 3,429,960 + +0.3 3,429,960 + -1.0 3,429,967 - -1.2 3,429,970 - -0.5 3,429,972 - -0.6 3,429,972 - -0.2 3,429,977 - +0.2 3,429,996 - +0.7 3,429,996 - +1.0 3,430,016 + +0.2 3,430,021 + -0.3 3,430,024 - -0.5 3,430,025 + +1.3 3,430,084 + smg b3284 0.15 +1.4 3,430,086 + smg b3284 0.15 -0.2 3,430,113 - smg b3284 0.21 +0.7 3,430,136 - smg b3284 0.26 -0.9 3,430,136 - smg b3284 0.26 -0.2 3,430,137 - smg b3284 0.26 -0.5 3,430,148 - smg b3284 0.28 -0.6 3,430,205 - smg b3284 0.41 -0.0 3,430,247 - smg b3284 0.49 +0.0 3,430,249 + smg b3284 0.50 +0.4 3,430,249 + smg b3284 0.50 +0.2 3,430,266 - smg b3284 0.53 +0.5 3,430,272 - smg b3284 0.55 +0.2 3,430,283 - smg b3284 0.57 -0.2 3,430,289 + smg b3284 0.58 +1.2 3,430,362 + smg b3284 0.74 -0.3 3,430,362 + smg b3284 0.74 -0.8 3,430,372 - smg b3284 0.76 +0.4 3,430,415 + smg b3284 0.85 -0.5 3,430,423 - smg b3284 0.86 +0.0 3,430,432 + smg b3284 0.88 -0.0 3,430,432 + smg b3284 0.88 +0.1 3,430,432 + smg b3284 0.88 +0.3 3,430,481 + +0.3 3,430,490 - -0.0 3,430,491 + +0.2 3,430,512 + +0.4 3,430,513 + +0.1 3,430,515 + -0.8 3,430,518 + -1.2 3,430,518 + +1.7 3,430,523 - -0.3 3,430,523 - -1.7 3,430,525 + -0.3 3,430,531 - +0.4 3,430,548 - +0.0 3,430,568 - -0.0 3,430,568 - +0.0 3,430,570 - +0.4 3,430,583 - smf b4473 0.11 -0.2 3,430,590 - smf b4473 0.12 +1.0 3,430,595 + smf b4473 0.12 +0.4 3,430,595 + smf b4473 0.12 +0.2 3,430,602 - smf b4473 0.13 -0.7 3,430,616 + smf b4473 0.14 -0.0 3,430,616 + smf b4473 0.14 +0.0 3,430,616 - smf b4473 0.14 +0.7 3,430,637 - smf b4473 0.16 -1.2 3,430,671 + smf b4473 0.19 +3.2 3,430,793 + smf b4473 0.30 -0.2 3,430,801 - smf b4473 0.30 +0.4 3,430,815 - smf b4473 0.32 -0.2 3,430,828 - smf b4473 0.33 +0.0 3,430,828 - smf b4473 0.33 +0.7 3,430,871 + smf b4473 0.37 +0.1 3,430,876 + smf b4473 0.37 -0.9 3,430,876 - smf b4473 0.37 +0.1 3,430,881 - smf b4473 0.38 -1.0 3,430,891 + smf b4473 0.38 +0.3 3,430,891 + smf b4473 0.38 +0.4 3,430,921 + smf b4473 0.41 -0.4 3,430,921 + smf b4473 0.41 +0.6 3,430,927 - smf b4473 0.42 -1.2 3,430,944 - smf b4473 0.43 +0.8 3,430,944 - smf b4473 0.43 -0.4 3,430,980 - smf b4473 0.46 -0.6 3,430,984 - smf b4473 0.47 +1.3 3,431,013 + smf b4473 0.49 +0.5 3,431,017 + smf b4473 0.50 -0.8 3,431,018 + smf b4473 0.50 +0.4 3,431,065 + smf b4473 0.54 -0.2 3,431,073 - smf b4473 0.55 -0.3 3,431,079 + smf b4473 0.55 +0.3 3,431,126 + smf b4473 0.59 -1.3 3,431,134 - smf b4473 0.60 -0.2 3,431,134 - smf b4473 0.60 -0.2 3,431,221 + smf b4473 0.68 -2.4 3,431,373 + smf b4473 0.81 +0.8 3,431,376 - smf b4473 0.82 +0.6 3,431,415 - smf b4473 0.85 -0.9 3,431,415 - smf b4473 0.85 +0.7 3,431,448 + smf b4473 0.88 -1.0 3,431,448 + smf b4473 0.88 +0.3 3,431,455 - smf b4473 0.89 +0.1 3,431,455 - smf b4473 0.89 +0.4 3,431,468 + smf b4473 0.90 +0.2 3,431,474 - +1.7 3,431,482 + -0.2
Or see this region's nucleotide sequence