Strain Fitness in Escherichia coli BW25113 around b2594

Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntclpB and ryfD overlap by 21 nucleotidesryfD and yfiH are separated by 7 nucleotidesyfiH and rluD overlap by 4 nucleotidesrluD and yfiO are separated by 134 nucleotides b2592: clpB - protein disaggregation chaperone (NCBI), at 2,729,622 to 2,732,195 clpB b4609: ryfD - Novel sRNA, function unknown (RefSeq), at 2,732,175 to 2,732,317 ryfD b2593: yfiH - hypothetical protein (NCBI), at 2,732,325 to 2,733,056 yfiH b2594: rluD - 23S rRNA pseudouridine synthase (NCBI), at 2,733,053 to 2,734,033 rluD b2595: yfiO - predicted lipoprotein (NCBI), at 2,734,168 to 2,734,905 yfiO Position (kb) 2733 2734 2735Strain fitness (log2 ratio) -1 0 1 2at 2732.140 kb on - strandat 2732.202 kb on + strand, within ryfDat 2732.205 kb on - strand, within ryfDat 2732.212 kb on + strand, within ryfDat 2732.212 kb on + strand, within ryfDat 2732.251 kb on + strand, within ryfDat 2732.277 kb on - strand, within ryfDat 2732.277 kb on - strand, within ryfDat 2732.323 kb on + strandat 2732.323 kb on + strandat 2732.456 kb on - strand, within yfiHat 2732.499 kb on - strand, within yfiHat 2732.499 kb on - strand, within yfiHat 2732.502 kb on - strand, within yfiHat 2732.502 kb on - strand, within yfiHat 2732.563 kb on - strand, within yfiHat 2732.563 kb on - strand, within yfiHat 2732.594 kb on + strand, within yfiHat 2732.602 kb on - strand, within yfiHat 2732.648 kb on + strand, within yfiHat 2732.659 kb on + strand, within yfiHat 2732.669 kb on + strand, within yfiHat 2732.748 kb on + strand, within yfiHat 2732.756 kb on - strand, within yfiHat 2732.763 kb on - strand, within yfiHat 2732.763 kb on - strand, within yfiHat 2732.778 kb on + strand, within yfiHat 2732.813 kb on - strand, within yfiHat 2732.813 kb on - strand, within yfiHat 2732.852 kb on - strand, within yfiHat 2732.854 kb on - strand, within yfiHat 2732.926 kb on + strand, within yfiHat 2732.943 kb on - strand, within yfiHat 2732.969 kb on - strand, within yfiHat 2732.969 kb on - strand, within yfiHat 2732.994 kb on + strandat 2733.052 kb on + strandat 2733.060 kb on - strandat 2733.062 kb on - strandat 2733.072 kb on + strandat 2733.079 kb on - strandat 2733.079 kb on - strandat 2734.040 kb on - strandat 2734.040 kb on - strandat 2734.042 kb on - strandat 2734.078 kb on + strandat 2734.095 kb on + strandat 2734.095 kb on + strandat 2734.103 kb on - strandat 2734.165 kb on + strandat 2734.833 kb on + strandat 2734.848 kb on - strandat 2734.979 kb on + strandat 2734.996 kb on - strandat 2734.996 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM
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2,732,140 - -0.7
2,732,202 + ryfD b4609 0.19 -0.6
2,732,205 - ryfD b4609 0.21 -0.1
2,732,212 + ryfD b4609 0.26 -1.3
2,732,212 + ryfD b4609 0.26 -1.2
2,732,251 + ryfD b4609 0.53 +0.2
2,732,277 - ryfD b4609 0.71 +2.2
2,732,277 - ryfD b4609 0.71 -0.8
2,732,323 + +1.4
2,732,323 + -0.7
2,732,456 - yfiH b2593 0.18 +1.2
2,732,499 - yfiH b2593 0.24 -0.5
2,732,499 - yfiH b2593 0.24 -1.2
2,732,502 - yfiH b2593 0.24 +1.6
2,732,502 - yfiH b2593 0.24 +0.2
2,732,563 - yfiH b2593 0.33 +1.2
2,732,563 - yfiH b2593 0.33 +1.2
2,732,594 + yfiH b2593 0.37 -0.1
2,732,602 - yfiH b2593 0.38 -0.2
2,732,648 + yfiH b2593 0.44 +0.1
2,732,659 + yfiH b2593 0.46 -1.4
2,732,669 + yfiH b2593 0.47 +0.1
2,732,748 + yfiH b2593 0.58 -0.0
2,732,756 - yfiH b2593 0.59 -1.1
2,732,763 - yfiH b2593 0.60 +1.2
2,732,763 - yfiH b2593 0.60 +0.2
2,732,778 + yfiH b2593 0.62 -0.6
2,732,813 - yfiH b2593 0.67 +0.3
2,732,813 - yfiH b2593 0.67 -0.1
2,732,852 - yfiH b2593 0.72 +0.3
2,732,854 - yfiH b2593 0.72 -0.1
2,732,926 + yfiH b2593 0.82 +1.6
2,732,943 - yfiH b2593 0.84 -0.4
2,732,969 - yfiH b2593 0.88 +0.4
2,732,969 - yfiH b2593 0.88 -0.2
2,732,994 + +0.2
2,733,052 + +0.0
2,733,060 - -0.4
2,733,062 - -0.2
2,733,072 + +0.9
2,733,079 - -0.3
2,733,079 - +0.8
2,734,040 - +0.2
2,734,040 - -1.3
2,734,042 - +0.7
2,734,078 + +1.2
2,734,095 + +0.7
2,734,095 + +0.9
2,734,103 - -0.2
2,734,165 + -0.8
2,734,833 + +1.7
2,734,848 - -0.4
2,734,979 + -0.3
2,734,996 - +0.5
2,734,996 - -0.4

Or see this region's nucleotide sequence