Strain Fitness in Escherichia coli BW25113 around b2585

Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfiQ and pssA are separated by 113 nucleotidespssA and yfiM are separated by 45 nucleotidesyfiM and kgtP overlap by 4 nucleotides b2584: yfiQ - fused predicted acyl-CoA synthetase: NAD(P)-binding subunit/ATP-binding subunit (NCBI), at 2,717,975 to 2,720,635 yfiQ b2585: pssA - phosphatidylserine synthase; phospholipid synthesis (VIMSS), at 2,720,749 to 2,722,104 pssA b2586: yfiM - predicted protein (RefSeq), at 2,722,150 to 2,722,473 yfiM b2587: kgtP - alpha-ketoglutarate transporter (NCBI), at 2,722,470 to 2,723,768 kgtP Position (kb) 2720 2721 2722 2723Strain fitness (log2 ratio) -2 -1 0 1at 2719.852 kb on - strand, within yfiQat 2719.931 kb on - strand, within yfiQat 2720.013 kb on + strand, within yfiQat 2720.013 kb on + strand, within yfiQat 2720.093 kb on - strand, within yfiQat 2720.105 kb on - strand, within yfiQat 2720.235 kb on - strand, within yfiQat 2720.288 kb on - strand, within yfiQat 2720.288 kb on - strand, within yfiQat 2720.368 kb on + strand, within yfiQat 2720.434 kb on - strandat 2720.438 kb on + strandat 2720.440 kb on - strandat 2720.440 kb on - strandat 2720.545 kb on + strandat 2720.545 kb on + strandat 2720.642 kb on - strandat 2721.348 kb on + strand, within pssAat 2722.110 kb on + strandat 2722.113 kb on - strandat 2722.115 kb on + strandat 2722.171 kb on + strandat 2722.227 kb on + strand, within yfiMat 2722.332 kb on + strand, within yfiMat 2722.387 kb on - strand, within yfiMat 2722.415 kb on + strand, within yfiMat 2722.435 kb on + strand, within yfiMat 2722.539 kb on - strandat 2722.539 kb on - strandat 2722.557 kb on + strandat 2722.557 kb on + strandat 2722.570 kb on + strandat 2722.604 kb on - strand, within kgtPat 2722.604 kb on - strand, within kgtPat 2722.633 kb on + strand, within kgtPat 2722.641 kb on - strand, within kgtPat 2722.783 kb on + strand, within kgtPat 2722.801 kb on + strand, within kgtPat 2722.943 kb on + strand, within kgtPat 2722.943 kb on + strand, within kgtPat 2723.060 kb on - strand, within kgtPat 2723.060 kb on - strand, within kgtP

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM
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2,719,852 - yfiQ b2584 0.71 +0.2
2,719,931 - yfiQ b2584 0.74 +0.6
2,720,013 + yfiQ b2584 0.77 +1.2
2,720,013 + yfiQ b2584 0.77 +0.2
2,720,093 - yfiQ b2584 0.80 -1.3
2,720,105 - yfiQ b2584 0.80 +0.7
2,720,235 - yfiQ b2584 0.85 -1.8
2,720,288 - yfiQ b2584 0.87 +0.6
2,720,288 - yfiQ b2584 0.87 -0.4
2,720,368 + yfiQ b2584 0.90 +1.4
2,720,434 - -0.2
2,720,438 + +1.7
2,720,440 - +0.6
2,720,440 - -1.4
2,720,545 + +1.2
2,720,545 + +1.7
2,720,642 - +0.2
2,721,348 + pssA b2585 0.44 +0.2
2,722,110 + -0.2
2,722,113 - -1.5
2,722,115 + -1.5
2,722,171 + +0.8
2,722,227 + yfiM b2586 0.24 +0.9
2,722,332 + yfiM b2586 0.56 -0.8
2,722,387 - yfiM b2586 0.73 +1.2
2,722,415 + yfiM b2586 0.82 +0.3
2,722,435 + yfiM b2586 0.88 -0.5
2,722,539 - -1.6
2,722,539 - -2.0
2,722,557 + +0.4
2,722,557 + +0.6
2,722,570 + -1.0
2,722,604 - kgtP b2587 0.10 -0.2
2,722,604 - kgtP b2587 0.10 +1.2
2,722,633 + kgtP b2587 0.13 -0.4
2,722,641 - kgtP b2587 0.13 +0.3
2,722,783 + kgtP b2587 0.24 +1.7
2,722,801 + kgtP b2587 0.25 +0.9
2,722,943 + kgtP b2587 0.36 +1.5
2,722,943 + kgtP b2587 0.36 +0.3
2,723,060 - kgtP b2587 0.45 +0.4
2,723,060 - kgtP b2587 0.45 -0.2

Or see this region's nucleotide sequence