Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yebB and ruvC are separated by 1 nucleotides ruvC and yebC are separated by 34 nucleotides yebC and nudB are separated by 28 nucleotides nudB and aspS are separated by 117 nucleotides
b1862: yebB - orf, hypothetical protein (VIMSS), at 1,944,275 to 1,944,877
yebB
b1863: ruvC - Holliday junction resolvase (NCBI), at 1,944,879 to 1,945,400
ruvC
b1864: yebC - hypothetical protein (NCBI), at 1,945,435 to 1,946,175
yebC
b1865: nudB - dATP pyrophosphohydrolase (NCBI), at 1,946,204 to 1,946,656
nudB
b1866: aspS - aspartyl-tRNA synthetase (NCBI), at 1,946,774 to 1,948,546
aspS
Position (kb)
1945
1946
1947 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1944.511 kb on - strand, within yebB at 1944.560 kb on - strand, within yebB at 1944.582 kb on + strand, within yebB at 1944.622 kb on + strand, within yebB at 1944.625 kb on - strand, within yebB at 1944.625 kb on - strand, within yebB at 1944.625 kb on - strand, within yebB at 1944.669 kb on - strand, within yebB at 1944.674 kb on - strand, within yebB at 1944.723 kb on - strand, within yebB at 1944.818 kb on + strand at 1944.818 kb on + strand at 1944.826 kb on - strand at 1944.826 kb on - strand at 1944.829 kb on + strand at 1944.846 kb on - strand at 1944.885 kb on - strand at 1944.885 kb on - strand at 1944.895 kb on - strand at 1944.908 kb on + strand at 1944.916 kb on - strand at 1945.037 kb on - strand, within ruvC at 1945.078 kb on - strand, within ruvC at 1945.219 kb on + strand, within ruvC at 1945.255 kb on - strand, within ruvC at 1945.260 kb on - strand, within ruvC at 1945.307 kb on + strand, within ruvC at 1945.323 kb on - strand, within ruvC at 1945.396 kb on - strand at 1945.455 kb on + strand at 1945.524 kb on - strand, within yebC at 1945.557 kb on - strand, within yebC at 1945.557 kb on - strand, within yebC at 1945.623 kb on - strand, within yebC at 1945.623 kb on - strand, within yebC at 1945.640 kb on - strand, within yebC at 1945.640 kb on - strand, within yebC at 1945.652 kb on + strand, within yebC at 1945.663 kb on - strand, within yebC at 1945.726 kb on - strand, within yebC at 1945.975 kb on + strand, within yebC at 1945.975 kb on + strand, within yebC at 1945.997 kb on + strand, within yebC at 1946.123 kb on - strand at 1946.247 kb on + strand at 1946.247 kb on + strand at 1946.435 kb on + strand, within nudB at 1946.471 kb on - strand, within nudB at 1946.471 kb on - strand, within nudB at 1946.636 kb on + strand at 1946.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 1,944,511 - yebB b1862 0.39 -0.7 1,944,560 - yebB b1862 0.47 -0.6 1,944,582 + yebB b1862 0.51 +0.5 1,944,622 + yebB b1862 0.58 -0.3 1,944,625 - yebB b1862 0.58 +0.3 1,944,625 - yebB b1862 0.58 +0.1 1,944,625 - yebB b1862 0.58 +0.7 1,944,669 - yebB b1862 0.65 -0.3 1,944,674 - yebB b1862 0.66 -0.6 1,944,723 - yebB b1862 0.74 -0.6 1,944,818 + +2.3 1,944,818 + +2.3 1,944,826 - +1.1 1,944,826 - +1.7 1,944,829 + +1.3 1,944,846 - -0.6 1,944,885 - -0.5 1,944,885 - +0.2 1,944,895 - -0.2 1,944,908 + -0.8 1,944,916 - +0.6 1,945,037 - ruvC b1863 0.30 -1.3 1,945,078 - ruvC b1863 0.38 -0.8 1,945,219 + ruvC b1863 0.65 -0.4 1,945,255 - ruvC b1863 0.72 +0.2 1,945,260 - ruvC b1863 0.73 -0.3 1,945,307 + ruvC b1863 0.82 -3.3 1,945,323 - ruvC b1863 0.85 -1.6 1,945,396 - -1.3 1,945,455 + -1.5 1,945,524 - yebC b1864 0.12 -1.2 1,945,557 - yebC b1864 0.16 -1.1 1,945,557 - yebC b1864 0.16 -0.5 1,945,623 - yebC b1864 0.25 +0.0 1,945,623 - yebC b1864 0.25 -0.7 1,945,640 - yebC b1864 0.28 -0.8 1,945,640 - yebC b1864 0.28 -0.7 1,945,652 + yebC b1864 0.29 -1.3 1,945,663 - yebC b1864 0.31 +0.5 1,945,726 - yebC b1864 0.39 -0.8 1,945,975 + yebC b1864 0.73 -1.1 1,945,975 + yebC b1864 0.73 +0.7 1,945,997 + yebC b1864 0.76 -0.7 1,946,123 - +0.7 1,946,247 + +3.9 1,946,247 + +3.7 1,946,435 + nudB b1865 0.51 +3.3 1,946,471 - nudB b1865 0.59 +4.0 1,946,471 - nudB b1865 0.59 +4.4 1,946,636 + +1.1 1,946,644 - +1.6
Or see this region's nucleotide sequence