Strain Fitness in Escherichia coli BW25113 around b1210

Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntispE and lolB overlap by 1 nucleotideslolB and hemA are separated by 213 nucleotideshemA and prfA are separated by 41 nucleotides b1208: ispE - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (NCBI), at 1,261,249 to 1,262,100 ispE b1209: lolB - outer membrane lipoprotein LolB precursor (NCBI), at 1,262,100 to 1,262,723 lolB b1210: hemA - glutamyl-tRNA reductase (NCBI), at 1,262,937 to 1,264,193 hemA b1211: prfA - peptide chain release factor 1 (NCBI), at 1,264,235 to 1,265,317 prfA Position (kb) 1262 1263 1264 1265Strain fitness (log2 ratio) -1 0 1at 1262.885 kb on + strandat 1262.934 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM
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1,262,885 + -1.2
1,262,934 + -1.2

Or see this region's nucleotide sequence