Strain Fitness in Escherichia coli BW25113 around b1086

Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrne and b1085 are separated by 134 nucleotidesb1085 and rluC are separated by 117 nucleotidesrluC and yceF are separated by 111 nucleotidesyceF and sraB overlap by 7 nucleotidessraB and yceD are separated by 36 nucleotides b1084: rne - fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI), at 1,140,405 to 1,143,590 rne b1085: b1085 - orf, hypothetical protein (VIMSS), at 1,143,725 to 1,144,045 b1085 b1086: rluC - 23S rRNA pseudouridylate synthase (NCBI), at 1,144,163 to 1,145,122 rluC b1087: yceF - orf, hypothetical protein (VIMSS), at 1,145,234 to 1,145,818 yceF b4418: sraB - unknown RNA (NCBI), at 1,145,812 to 1,145,980 sraB b1088: yceD - hypothetical protein (NCBI), at 1,146,017 to 1,146,538 yceD Position (kb) 1144 1145 1146Strain fitness (log2 ratio) -1 0 1 2 3at 1143.725 kb on - strandat 1143.731 kb on - strandat 1143.821 kb on - strand, within b1085at 1143.969 kb on - strand, within b1085at 1143.969 kb on - strand, within b1085at 1144.032 kb on - strandat 1144.039 kb on + strandat 1144.083 kb on + strandat 1144.109 kb on - strandat 1144.145 kb on - strandat 1144.295 kb on + strand, within rluCat 1144.438 kb on - strand, within rluCat 1144.509 kb on - strand, within rluCat 1144.575 kb on - strand, within rluCat 1144.592 kb on + strand, within rluCat 1144.610 kb on - strand, within rluCat 1144.655 kb on + strand, within rluCat 1144.655 kb on + strand, within rluCat 1144.699 kb on + strand, within rluCat 1144.699 kb on + strand, within rluCat 1144.867 kb on + strand, within rluCat 1144.867 kb on + strand, within rluCat 1144.899 kb on + strand, within rluCat 1144.988 kb on + strand, within rluCat 1144.988 kb on + strand, within rluCat 1144.998 kb on + strand, within rluCat 1144.998 kb on + strand, within rluCat 1145.011 kb on - strand, within rluCat 1145.011 kb on - strand, within rluCat 1145.035 kb on + strandat 1145.058 kb on - strandat 1145.145 kb on - strandat 1145.279 kb on - strandat 1145.381 kb on + strand, within yceFat 1145.381 kb on + strand, within yceFat 1145.381 kb on + strand, within yceFat 1145.381 kb on + strand, within yceFat 1145.389 kb on - strand, within yceFat 1145.389 kb on - strand, within yceFat 1145.389 kb on - strand, within yceFat 1145.395 kb on + strand, within yceFat 1145.511 kb on - strand, within yceFat 1145.516 kb on - strand, within yceFat 1145.516 kb on - strand, within yceFat 1145.576 kb on - strand, within yceFat 1145.610 kb on - strand, within yceFat 1145.614 kb on - strand, within yceFat 1145.673 kb on - strand, within yceFat 1145.724 kb on - strand, within yceFat 1145.734 kb on - strand, within yceFat 1145.734 kb on - strand, within yceFat 1145.841 kb on + strand, within sraBat 1145.847 kb on - strand, within sraBat 1146.042 kb on + strandat 1146.052 kb on + strandat 1146.096 kb on + strand, within yceDat 1146.096 kb on + strand, within yceDat 1146.117 kb on + strand, within yceD

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM
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1,143,725 - +0.0
1,143,731 - +0.0
1,143,821 - b1085 b1085 0.30 -1.2
1,143,969 - b1085 b1085 0.76 +0.7
1,143,969 - b1085 b1085 0.76 +0.0
1,144,032 - +0.3
1,144,039 + +1.0
1,144,083 + -0.2
1,144,109 - +1.0
1,144,145 - -0.6
1,144,295 + rluC b1086 0.14 -0.2
1,144,438 - rluC b1086 0.29 -0.9
1,144,509 - rluC b1086 0.36 +0.3
1,144,575 - rluC b1086 0.43 -0.2
1,144,592 + rluC b1086 0.45 -0.6
1,144,610 - rluC b1086 0.47 +0.7
1,144,655 + rluC b1086 0.51 -1.2
1,144,655 + rluC b1086 0.51 +1.2
1,144,699 + rluC b1086 0.56 -1.7
1,144,699 + rluC b1086 0.56 -1.0
1,144,867 + rluC b1086 0.73 +0.3
1,144,867 + rluC b1086 0.73 +1.8
1,144,899 + rluC b1086 0.77 +0.8
1,144,988 + rluC b1086 0.86 +0.7
1,144,988 + rluC b1086 0.86 +0.3
1,144,998 + rluC b1086 0.87 -0.7
1,144,998 + rluC b1086 0.87 +0.2
1,145,011 - rluC b1086 0.88 -0.4
1,145,011 - rluC b1086 0.88 +0.8
1,145,035 + -0.3
1,145,058 - -0.5
1,145,145 - +0.5
1,145,279 - -0.1
1,145,381 + yceF b1087 0.25 +0.2
1,145,381 + yceF b1087 0.25 +0.1
1,145,381 + yceF b1087 0.25 -0.2
1,145,381 + yceF b1087 0.25 +0.8
1,145,389 - yceF b1087 0.26 +3.4
1,145,389 - yceF b1087 0.26 +2.2
1,145,389 - yceF b1087 0.26 +0.3
1,145,395 + yceF b1087 0.28 +1.0
1,145,511 - yceF b1087 0.47 -0.4
1,145,516 - yceF b1087 0.48 +1.8
1,145,516 - yceF b1087 0.48 +1.7
1,145,576 - yceF b1087 0.58 +0.0
1,145,610 - yceF b1087 0.64 +0.4
1,145,614 - yceF b1087 0.65 -0.3
1,145,673 - yceF b1087 0.75 +2.4
1,145,724 - yceF b1087 0.84 +1.4
1,145,734 - yceF b1087 0.85 -0.4
1,145,734 - yceF b1087 0.85 +0.4
1,145,841 + sraB b4418 0.17 +0.7
1,145,847 - sraB b4418 0.21 -0.7
1,146,042 + +0.1
1,146,052 + +1.8
1,146,096 + yceD b1088 0.15 +0.8
1,146,096 + yceD b1088 0.15 -1.3
1,146,117 + yceD b1088 0.19 +0.4

Or see this region's nucleotide sequence