Experiment: Rhamnogalacturonan - from potato (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3141 and Atu3142 overlap by 4 nucleotides Atu3142 and Atu3143 are separated by 138 nucleotides Atu3143 and Atu3144 are separated by 89 nucleotides Atu3144 and Atu3145 are separated by 50 nucleotides
Atu3141: Atu3141 - 2-deoxy-D-gluconate 3-dehydrogenase, at 149,169 to 149,912
Atu3141
Atu3142: Atu3142 - 5-keto-4-deoxyuronate isomerase, at 149,909 to 150,733
Atu3142
Atu3143: Atu3143 - hypothetical protein, at 150,872 to 151,669
Atu3143
Atu3144: Atu3144 - GntR family transcriptional regulator, at 151,759 to 152,496
Atu3144
Atu3145: Atu3145 - pectin degradation protein, at 152,547 to 152,864
Atu3145
Position (kb)
150
151
152 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 149.920 kb on - strand at 149.964 kb on - strand at 149.978 kb on + strand at 150.173 kb on + strand, within Atu3142 at 150.173 kb on + strand, within Atu3142 at 150.201 kb on + strand, within Atu3142 at 150.201 kb on + strand, within Atu3142 at 150.201 kb on + strand, within Atu3142 at 150.209 kb on - strand, within Atu3142 at 150.209 kb on - strand, within Atu3142 at 150.214 kb on - strand, within Atu3142 at 150.261 kb on - strand, within Atu3142 at 150.291 kb on + strand, within Atu3142 at 150.325 kb on - strand, within Atu3142 at 150.351 kb on + strand, within Atu3142 at 150.359 kb on - strand, within Atu3142 at 150.359 kb on - strand, within Atu3142 at 150.363 kb on + strand, within Atu3142 at 150.373 kb on + strand, within Atu3142 at 150.381 kb on - strand, within Atu3142 at 150.422 kb on - strand, within Atu3142 at 150.564 kb on + strand, within Atu3142 at 150.564 kb on + strand, within Atu3142 at 150.572 kb on - strand, within Atu3142 at 150.572 kb on - strand, within Atu3142 at 150.589 kb on - strand, within Atu3142 at 150.604 kb on - strand, within Atu3142 at 150.606 kb on + strand, within Atu3142 at 150.606 kb on + strand, within Atu3142 at 150.606 kb on + strand, within Atu3142 at 150.614 kb on - strand, within Atu3142 at 150.614 kb on - strand, within Atu3142 at 150.614 kb on - strand, within Atu3142 at 150.614 kb on - strand, within Atu3142 at 150.630 kb on + strand, within Atu3142 at 150.675 kb on - strand at 150.817 kb on + strand at 150.817 kb on - strand at 150.835 kb on - strand at 150.835 kb on - strand at 150.837 kb on + strand at 150.930 kb on + strand at 150.970 kb on + strand, within Atu3143 at 150.992 kb on - strand, within Atu3143 at 151.065 kb on + strand, within Atu3143 at 151.073 kb on - strand, within Atu3143 at 151.128 kb on - strand, within Atu3143 at 151.164 kb on + strand, within Atu3143 at 151.172 kb on - strand, within Atu3143 at 151.223 kb on - strand, within Atu3143 at 151.247 kb on - strand, within Atu3143 at 151.337 kb on - strand, within Atu3143 at 151.454 kb on + strand, within Atu3143 at 151.546 kb on + strand, within Atu3143 at 151.546 kb on + strand, within Atu3143 at 151.554 kb on - strand, within Atu3143 at 151.570 kb on + strand, within Atu3143 at 151.621 kb on + strand at 151.756 kb on - strand at 151.775 kb on + strand at 151.789 kb on + strand at 151.797 kb on + strand at 151.800 kb on - strand at 151.805 kb on - strand at 151.950 kb on + strand, within Atu3144 at 151.965 kb on + strand, within Atu3144 at 152.044 kb on - strand, within Atu3144 at 152.207 kb on - strand, within Atu3144 at 152.404 kb on - strand, within Atu3144 at 152.422 kb on + strand, within Atu3144 at 152.445 kb on + strand at 152.476 kb on + strand at 152.523 kb on + strand at 152.639 kb on - strand, within Atu3145
Per-strain Table
Position Strand Gene LocusTag Fraction Rhamnogalacturonan - from potato (C) remove 149,920 - +1.8 149,964 - -0.1 149,978 + +0.3 150,173 + Atu3142 0.32 +0.3 150,173 + Atu3142 0.32 +0.3 150,201 + Atu3142 0.35 -0.5 150,201 + Atu3142 0.35 +1.4 150,201 + Atu3142 0.35 -0.1 150,209 - Atu3142 0.36 +0.4 150,209 - Atu3142 0.36 -0.6 150,214 - Atu3142 0.37 +1.4 150,261 - Atu3142 0.43 -0.2 150,291 + Atu3142 0.46 -0.7 150,325 - Atu3142 0.50 -0.1 150,351 + Atu3142 0.54 +0.6 150,359 - Atu3142 0.55 +0.5 150,359 - Atu3142 0.55 -1.8 150,363 + Atu3142 0.55 -0.4 150,373 + Atu3142 0.56 -0.3 150,381 - Atu3142 0.57 -0.6 150,422 - Atu3142 0.62 -0.8 150,564 + Atu3142 0.79 -0.2 150,564 + Atu3142 0.79 +0.6 150,572 - Atu3142 0.80 -0.6 150,572 - Atu3142 0.80 +0.0 150,589 - Atu3142 0.82 +0.8 150,604 - Atu3142 0.84 -0.2 150,606 + Atu3142 0.84 -0.4 150,606 + Atu3142 0.84 -0.3 150,606 + Atu3142 0.84 -0.2 150,614 - Atu3142 0.85 +1.1 150,614 - Atu3142 0.85 -0.3 150,614 - Atu3142 0.85 -0.6 150,614 - Atu3142 0.85 -0.2 150,630 + Atu3142 0.87 -1.5 150,675 - +0.1 150,817 + -2.6 150,817 - -3.1 150,835 - -4.4 150,835 - -3.8 150,837 + -1.8 150,930 + -5.0 150,970 + Atu3143 0.12 -4.0 150,992 - Atu3143 0.15 -3.4 151,065 + Atu3143 0.24 -0.8 151,073 - Atu3143 0.25 -3.5 151,128 - Atu3143 0.32 -5.3 151,164 + Atu3143 0.37 -2.3 151,172 - Atu3143 0.38 -4.8 151,223 - Atu3143 0.44 -3.1 151,247 - Atu3143 0.47 -2.1 151,337 - Atu3143 0.58 +1.8 151,454 + Atu3143 0.73 -5.8 151,546 + Atu3143 0.84 -3.1 151,546 + Atu3143 0.84 -1.8 151,554 - Atu3143 0.85 -3.0 151,570 + Atu3143 0.87 -2.9 151,621 + -3.0 151,756 - +0.7 151,775 + +0.7 151,789 + +1.0 151,797 + +1.2 151,800 - +0.2 151,805 - -0.1 151,950 + Atu3144 0.26 -1.4 151,965 + Atu3144 0.28 +0.2 152,044 - Atu3144 0.39 +0.0 152,207 - Atu3144 0.61 +1.8 152,404 - Atu3144 0.87 -0.3 152,422 + Atu3144 0.90 -0.4 152,445 + -0.2 152,476 + -0.1 152,523 + -0.1 152,639 - Atu3145 0.29 -0.2
Or see this region's nucleotide sequence