Strain Fitness in Escherichia coli BW25113 around b2950

Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyqgE and yqgF overlap by 1 nucleotidesyqgF and yggR are separated by 183 nucleotidesyggR and yggS are separated by 17 nucleotidesyggS and yggT are separated by 17 nucleotides b2948: yqgE - orf, hypothetical protein (VIMSS), at 3,090,959 to 3,091,522 yqgE b2949: yqgF - Holliday junction resolvase-like protein (NCBI), at 3,091,522 to 3,091,938 yqgF b2950: yggR - putative protein transport (VIMSS), at 3,092,122 to 3,093,102 yggR b2951: yggS - predicted enzyme (NCBI), at 3,093,120 to 3,093,824 yggS b2952: yggT - predicted inner membrane protein (NCBI), at 3,093,842 to 3,094,408 yggT Position (kb) 3092 3093 3094Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3091.140 kb on + strand, within yqgEat 3091.140 kb on + strand, within yqgEat 3091.271 kb on - strand, within yqgEat 3091.271 kb on - strand, within yqgEat 3091.328 kb on + strand, within yqgEat 3091.534 kb on + strandat 3091.929 kb on + strandat 3091.942 kb on + strandat 3091.973 kb on - strandat 3091.973 kb on - strandat 3091.982 kb on - strandat 3092.008 kb on - strandat 3092.032 kb on + strandat 3092.032 kb on + strandat 3092.034 kb on + strandat 3092.053 kb on + strandat 3092.156 kb on - strandat 3092.156 kb on - strandat 3092.336 kb on - strand, within yggRat 3092.338 kb on + strand, within yggRat 3092.338 kb on + strand, within yggRat 3092.357 kb on - strand, within yggRat 3092.395 kb on + strand, within yggRat 3092.398 kb on + strand, within yggRat 3092.525 kb on - strand, within yggRat 3092.671 kb on - strand, within yggRat 3092.671 kb on - strand, within yggRat 3092.780 kb on + strand, within yggRat 3092.927 kb on + strand, within yggRat 3092.927 kb on + strandat 3092.930 kb on - strand, within yggRat 3092.953 kb on + strand, within yggRat 3093.045 kb on + strandat 3093.052 kb on - strandat 3093.052 kb on - strandat 3093.325 kb on + strand, within yggSat 3093.345 kb on + strand, within yggSat 3093.345 kb on + strand, within yggSat 3093.377 kb on + strand, within yggSat 3093.416 kb on + strand, within yggSat 3093.416 kb on + strand, within yggSat 3093.416 kb on + strand, within yggSat 3093.604 kb on + strand, within yggSat 3093.604 kb on + strand, within yggSat 3093.675 kb on + strand, within yggSat 3093.729 kb on - strand, within yggSat 3093.855 kb on - strandat 3093.855 kb on - strandat 3093.883 kb on + strandat 3093.891 kb on - strandat 3093.930 kb on - strand, within yggTat 3093.930 kb on - strand, within yggTat 3093.940 kb on - strand, within yggTat 3094.022 kb on + strand, within yggTat 3094.067 kb on - strand, within yggTat 3094.089 kb on - strand, within yggTat 3094.089 kb on - strand, within yggT

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM
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3,091,140 + yqgE b2948 0.32 +0.1
3,091,140 + yqgE b2948 0.32 +0.0
3,091,271 - yqgE b2948 0.55 +1.0
3,091,271 - yqgE b2948 0.55 -0.6
3,091,328 + yqgE b2948 0.65 +0.3
3,091,534 + -1.1
3,091,929 + +0.0
3,091,942 + -0.1
3,091,973 - +0.3
3,091,973 - +0.4
3,091,982 - -0.2
3,092,008 - +0.4
3,092,032 + +0.0
3,092,032 + -0.3
3,092,034 + +0.2
3,092,053 + -0.5
3,092,156 - -0.9
3,092,156 - -1.2
3,092,336 - yggR b2950 0.22 +1.2
3,092,338 + yggR b2950 0.22 +0.7
3,092,338 + yggR b2950 0.22 +1.0
3,092,357 - yggR b2950 0.24 -0.9
3,092,395 + yggR b2950 0.28 -1.6
3,092,398 + yggR b2950 0.28 -0.4
3,092,525 - yggR b2950 0.41 +0.0
3,092,671 - yggR b2950 0.56 -1.0
3,092,671 - yggR b2950 0.56 -0.6
3,092,780 + yggR b2950 0.67 -0.6
3,092,927 + yggR b2950 0.82 -1.7
3,092,927 + +1.0
3,092,930 - yggR b2950 0.82 -0.1
3,092,953 + yggR b2950 0.85 -0.6
3,093,045 + -1.3
3,093,052 - -0.2
3,093,052 - -0.2
3,093,325 + yggS b2951 0.29 +2.8
3,093,345 + yggS b2951 0.32 +3.2
3,093,345 + yggS b2951 0.32 +3.0
3,093,377 + yggS b2951 0.36 +1.2
3,093,416 + yggS b2951 0.42 +0.4
3,093,416 + yggS b2951 0.42 +1.9
3,093,416 + yggS b2951 0.42 +2.3
3,093,604 + yggS b2951 0.69 +1.2
3,093,604 + yggS b2951 0.69 -0.2
3,093,675 + yggS b2951 0.79 +0.3
3,093,729 - yggS b2951 0.86 +2.8
3,093,855 - +1.0
3,093,855 - -1.8
3,093,883 + -0.3
3,093,891 - +0.2
3,093,930 - yggT b2952 0.16 +0.3
3,093,930 - yggT b2952 0.16 +1.0
3,093,940 - yggT b2952 0.17 +1.9
3,094,022 + yggT b2952 0.32 +0.1
3,094,067 - yggT b2952 0.40 +0.7
3,094,089 - yggT b2952 0.44 +0.0
3,094,089 - yggT b2952 0.44 +1.2

Or see this region's nucleotide sequence