Strain Fitness in Escherichia coli BW25113 around b2393

Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmntH and nupC are separated by 335 nucleotidesnupC and yi81_3 are separated by 86 nucleotides b2392: mntH - manganese transport protein MntH (NCBI), at 2,509,490 to 2,510,728 mntH b2393: nupC - nucleoside (except guanosine) transporter (NCBI), at 2,511,064 to 2,512,266 nupC b2394: yi81_3 - IS186 hypothetical protein (VIMSS), at 2,512,353 to 2,513,465 _3 Position (kb) 2511 2512 2513Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 2510.130 kb on + strand, within mntHat 2510.130 kb on + strand, within mntHat 2510.198 kb on + strand, within mntHat 2510.243 kb on + strand, within mntHat 2510.243 kb on + strand, within mntHat 2510.251 kb on - strand, within mntHat 2510.435 kb on - strand, within mntHat 2510.454 kb on - strand, within mntHat 2510.533 kb on - strand, within mntHat 2510.595 kb on - strand, within mntHat 2510.607 kb on - strandat 2510.643 kb on - strandat 2510.708 kb on - strandat 2510.892 kb on + strandat 2510.921 kb on - strandat 2510.921 kb on - strandat 2510.972 kb on + strandat 2510.972 kb on + strandat 2510.974 kb on + strandat 2510.974 kb on + strandat 2510.974 kb on + strandat 2511.001 kb on + strandat 2511.001 kb on + strandat 2511.024 kb on - strandat 2511.061 kb on + strandat 2511.061 kb on + strandat 2511.118 kb on + strandat 2511.169 kb on - strandat 2511.250 kb on - strand, within nupCat 2511.284 kb on + strand, within nupCat 2511.365 kb on + strand, within nupCat 2511.504 kb on + strand, within nupCat 2511.504 kb on + strand, within nupCat 2511.564 kb on + strand, within nupCat 2511.623 kb on - strand, within nupCat 2511.623 kb on - strand, within nupCat 2511.690 kb on + strand, within nupCat 2511.730 kb on - strand, within nupCat 2511.918 kb on + strand, within nupCat 2511.930 kb on - strand, within nupCat 2511.993 kb on - strand, within nupCat 2511.995 kb on + strand, within nupCat 2511.995 kb on + strand, within nupCat 2512.099 kb on - strand, within nupCat 2512.099 kb on - strand, within nupCat 2512.222 kb on - strandat 2512.222 kb on - strandat 2512.227 kb on - strandat 2512.228 kb on + strandat 2512.331 kb on - strandat 2512.331 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM
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2,510,130 + mntH b2392 0.52 -0.2
2,510,130 + mntH b2392 0.52 +0.2
2,510,198 + mntH b2392 0.57 -0.8
2,510,243 + mntH b2392 0.61 +1.6
2,510,243 + mntH b2392 0.61 -1.5
2,510,251 - mntH b2392 0.61 -0.1
2,510,435 - mntH b2392 0.76 +0.8
2,510,454 - mntH b2392 0.78 -0.4
2,510,533 - mntH b2392 0.84 -0.7
2,510,595 - mntH b2392 0.89 -0.9
2,510,607 - +0.1
2,510,643 - -0.8
2,510,708 - +0.4
2,510,892 + -2.2
2,510,921 - +0.5
2,510,921 - +0.3
2,510,972 + -1.3
2,510,972 + -0.5
2,510,974 + -0.8
2,510,974 + -1.8
2,510,974 + +0.1
2,511,001 + -1.2
2,511,001 + +0.6
2,511,024 - +3.4
2,511,061 + +3.2
2,511,061 + +3.4
2,511,118 + +3.7
2,511,169 - +3.6
2,511,250 - nupC b2393 0.15 +3.2
2,511,284 + nupC b2393 0.18 +3.6
2,511,365 + nupC b2393 0.25 +3.4
2,511,504 + nupC b2393 0.37 +5.3
2,511,504 + nupC b2393 0.37 +3.8
2,511,564 + nupC b2393 0.42 +4.1
2,511,623 - nupC b2393 0.46 +3.2
2,511,623 - nupC b2393 0.46 +3.8
2,511,690 + nupC b2393 0.52 +3.5
2,511,730 - nupC b2393 0.55 +1.6
2,511,918 + nupC b2393 0.71 +3.7
2,511,930 - nupC b2393 0.72 +4.5
2,511,993 - nupC b2393 0.77 +2.1
2,511,995 + nupC b2393 0.77 +4.1
2,511,995 + nupC b2393 0.77 +3.8
2,512,099 - nupC b2393 0.86 +3.1
2,512,099 - nupC b2393 0.86 +4.1
2,512,222 - +2.8
2,512,222 - +3.8
2,512,227 - +3.6
2,512,228 + +3.7
2,512,331 - +3.4
2,512,331 - +3.3

Or see this region's nucleotide sequence