Strain Fitness in Escherichia coli BW25113 around b2390

Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfeO and ypeC are separated by 114 nucleotidesypeC and mntH are separated by 140 nucleotides b2389: yfeO - hypothetical protein (NCBI), at 2,507,652 to 2,508,908 yfeO b2390: ypeC - hypothetical protein (NCBI), at 2,509,023 to 2,509,349 ypeC b2392: mntH - manganese transport protein MntH (NCBI), at 2,509,490 to 2,510,728 mntH Position (kb) 2509 2510Strain fitness (log2 ratio) -2 -1 0 1 2at 2508.302 kb on + strand, within yfeOat 2508.328 kb on + strand, within yfeOat 2508.368 kb on - strand, within yfeOat 2508.377 kb on + strand, within yfeOat 2508.377 kb on + strand, within yfeOat 2508.377 kb on + strand, within yfeOat 2508.457 kb on + strand, within yfeOat 2508.457 kb on + strand, within yfeOat 2508.465 kb on - strand, within yfeOat 2508.518 kb on - strand, within yfeOat 2508.537 kb on + strand, within yfeOat 2508.537 kb on + strand, within yfeOat 2508.737 kb on - strand, within yfeOat 2508.780 kb on + strand, within yfeOat 2508.791 kb on - strandat 2508.793 kb on - strandat 2508.793 kb on - strandat 2508.799 kb on + strandat 2508.812 kb on + strandat 2508.812 kb on + strandat 2508.814 kb on + strandat 2508.817 kb on - strandat 2508.844 kb on - strandat 2508.950 kb on + strandat 2508.964 kb on - strandat 2509.096 kb on + strand, within ypeCat 2509.156 kb on - strand, within ypeCat 2509.174 kb on + strand, within ypeCat 2509.182 kb on - strand, within ypeCat 2509.182 kb on - strand, within ypeCat 2509.251 kb on - strand, within ypeCat 2509.251 kb on - strandat 2509.338 kb on - strandat 2509.338 kb on - strandat 2509.375 kb on - strandat 2509.403 kb on + strandat 2509.427 kb on - strandat 2509.553 kb on + strandat 2509.581 kb on + strandat 2509.704 kb on - strand, within mntHat 2509.711 kb on - strand, within mntHat 2509.711 kb on - strand, within mntHat 2509.772 kb on - strand, within mntHat 2509.809 kb on + strand, within mntHat 2509.811 kb on + strand, within mntHat 2509.839 kb on - strand, within mntHat 2509.875 kb on - strand, within mntHat 2509.957 kb on + strand, within mntHat 2509.967 kb on - strand, within mntHat 2510.018 kb on - strand, within mntHat 2510.018 kb on - strand, within mntHat 2510.130 kb on + strand, within mntHat 2510.130 kb on + strand, within mntHat 2510.198 kb on + strand, within mntHat 2510.243 kb on + strand, within mntHat 2510.243 kb on + strand, within mntHat 2510.251 kb on - strand, within mntH

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM
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2,508,302 + yfeO b2389 0.52 +0.6
2,508,328 + yfeO b2389 0.54 +0.3
2,508,368 - yfeO b2389 0.57 -0.8
2,508,377 + yfeO b2389 0.58 +0.2
2,508,377 + yfeO b2389 0.58 -0.2
2,508,377 + yfeO b2389 0.58 -0.3
2,508,457 + yfeO b2389 0.64 +1.2
2,508,457 + yfeO b2389 0.64 +2.4
2,508,465 - yfeO b2389 0.65 -0.2
2,508,518 - yfeO b2389 0.69 +0.4
2,508,537 + yfeO b2389 0.70 +0.5
2,508,537 + yfeO b2389 0.70 -0.2
2,508,737 - yfeO b2389 0.86 +0.9
2,508,780 + yfeO b2389 0.90 -1.2
2,508,791 - -0.4
2,508,793 - -0.6
2,508,793 - +2.0
2,508,799 + +0.7
2,508,812 + +0.3
2,508,812 + +0.6
2,508,814 + -0.2
2,508,817 - +0.7
2,508,844 - +1.2
2,508,950 + +0.2
2,508,964 - +1.1
2,509,096 + ypeC b2390 0.22 +0.2
2,509,156 - ypeC b2390 0.41 -0.4
2,509,174 + ypeC b2390 0.46 +0.3
2,509,182 - ypeC b2390 0.49 -0.2
2,509,182 - ypeC b2390 0.49 +1.6
2,509,251 - ypeC b2390 0.70 -1.1
2,509,251 - -0.2
2,509,338 - +0.7
2,509,338 - +0.8
2,509,375 - +0.6
2,509,403 + +0.6
2,509,427 - +0.2
2,509,553 + -0.8
2,509,581 + -0.4
2,509,704 - mntH b2392 0.17 -1.3
2,509,711 - mntH b2392 0.18 -1.2
2,509,711 - mntH b2392 0.18 -0.2
2,509,772 - mntH b2392 0.23 -2.0
2,509,809 + mntH b2392 0.26 -0.0
2,509,811 + mntH b2392 0.26 -1.5
2,509,839 - mntH b2392 0.28 -0.2
2,509,875 - mntH b2392 0.31 -0.8
2,509,957 + mntH b2392 0.38 -0.2
2,509,967 - mntH b2392 0.38 -1.8
2,510,018 - mntH b2392 0.43 -1.0
2,510,018 - mntH b2392 0.43 -1.5
2,510,130 + mntH b2392 0.52 -0.2
2,510,130 + mntH b2392 0.52 +0.2
2,510,198 + mntH b2392 0.57 -0.8
2,510,243 + mntH b2392 0.61 +1.6
2,510,243 + mntH b2392 0.61 -1.5
2,510,251 - mntH b2392 0.61 -0.1

Or see this region's nucleotide sequence