Strain Fitness in Escherichia coli BW25113 around b2342

Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfcX and yfcY overlap by 1 nucleotidesyfcY and b2343 are separated by 180 nucleotidesb2343 and fadL are separated by 371 nucleotides b2341: yfcX - fused enoyl-CoA hydratase and epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase (NCBI), at 2,455,037 to 2,457,181 yfcX b2342: yfcY - acetyl-CoA acetyltransferase (NCBI), at 2,457,181 to 2,458,491 yfcY b2343: b2343 - orf, hypothetical protein (VIMSS), at 2,458,672 to 2,458,956 b2343 b2344: fadL - long-chain fatty acid outer membrane transporter (RefSeq), at 2,459,328 to 2,460,668 fadL Position (kb) 2457 2458 2459Strain fitness (log2 ratio) -1 0 1 2at 2456.253 kb on + strand, within yfcXat 2456.253 kb on + strand, within yfcXat 2456.383 kb on + strand, within yfcXat 2456.402 kb on - strand, within yfcXat 2456.425 kb on + strand, within yfcXat 2456.425 kb on + strand, within yfcXat 2456.544 kb on + strand, within yfcXat 2456.614 kb on + strand, within yfcXat 2456.614 kb on + strand, within yfcXat 2456.617 kb on - strand, within yfcXat 2456.617 kb on - strand, within yfcXat 2456.663 kb on - strand, within yfcXat 2456.684 kb on + strand, within yfcXat 2456.747 kb on + strand, within yfcXat 2456.747 kb on + strand, within yfcXat 2456.771 kb on + strand, within yfcXat 2456.771 kb on + strand, within yfcXat 2457.130 kb on - strandat 2457.130 kb on - strandat 2457.241 kb on + strandat 2457.268 kb on - strandat 2457.326 kb on - strand, within yfcYat 2457.381 kb on + strand, within yfcYat 2457.473 kb on + strand, within yfcYat 2457.532 kb on + strand, within yfcYat 2457.532 kb on + strand, within yfcYat 2457.535 kb on - strand, within yfcYat 2457.539 kb on - strand, within yfcYat 2457.561 kb on + strand, within yfcYat 2457.636 kb on - strand, within yfcYat 2457.876 kb on + strand, within yfcYat 2458.221 kb on - strand, within yfcYat 2458.233 kb on - strand, within yfcYat 2458.233 kb on - strand, within yfcYat 2458.296 kb on + strand, within yfcYat 2458.452 kb on + strandat 2458.470 kb on - strandat 2458.496 kb on - strandat 2458.654 kb on + strandat 2458.859 kb on - strand, within b2343at 2458.859 kb on - strand, within b2343at 2459.436 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM
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2,456,253 + yfcX b2341 0.57 +0.6
2,456,253 + yfcX b2341 0.57 -0.5
2,456,383 + yfcX b2341 0.63 -0.2
2,456,402 - yfcX b2341 0.64 +0.5
2,456,425 + yfcX b2341 0.65 +0.4
2,456,425 + yfcX b2341 0.65 -0.5
2,456,544 + yfcX b2341 0.70 -1.5
2,456,614 + yfcX b2341 0.74 -0.4
2,456,614 + yfcX b2341 0.74 +0.1
2,456,617 - yfcX b2341 0.74 +0.4
2,456,617 - yfcX b2341 0.74 +0.3
2,456,663 - yfcX b2341 0.76 +0.9
2,456,684 + yfcX b2341 0.77 +0.5
2,456,747 + yfcX b2341 0.80 -0.3
2,456,747 + yfcX b2341 0.80 +0.7
2,456,771 + yfcX b2341 0.81 +0.8
2,456,771 + yfcX b2341 0.81 +0.8
2,457,130 - +0.1
2,457,130 - -0.4
2,457,241 + -0.2
2,457,268 - +0.7
2,457,326 - yfcY b2342 0.11 +2.5
2,457,381 + yfcY b2342 0.15 +1.7
2,457,473 + yfcY b2342 0.22 +1.5
2,457,532 + yfcY b2342 0.27 -1.7
2,457,532 + yfcY b2342 0.27 -0.6
2,457,535 - yfcY b2342 0.27 +0.3
2,457,539 - yfcY b2342 0.27 +1.2
2,457,561 + yfcY b2342 0.29 +0.5
2,457,636 - yfcY b2342 0.35 +1.2
2,457,876 + yfcY b2342 0.53 +0.1
2,458,221 - yfcY b2342 0.79 +0.4
2,458,233 - yfcY b2342 0.80 -0.8
2,458,233 - yfcY b2342 0.80 -0.4
2,458,296 + yfcY b2342 0.85 +0.0
2,458,452 + -0.2
2,458,470 - -0.0
2,458,496 - -0.6
2,458,654 + -1.5
2,458,859 - b2343 b2343 0.66 -0.4
2,458,859 - b2343 b2343 0.66 -0.0
2,459,436 + +0.8

Or see this region's nucleotide sequence