Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydfC and dicF are separated by 340 nucleotides dicF and dicB are separated by 174 nucleotides dicB and ydfD overlap by 4 nucleotides ydfD and ydfE are separated by 92 nucleotides ydfE and insD are separated by 2 nucleotides
b1573: ydfC - Qin prophage; predicted protein (RefSeq), at 1,646,847 to 1,647,065
ydfC
b1574: dicF - antisense RNA DicF (NCBI), at 1,647,406 to 1,647,458
dicF
b1575: dicB - Qin prophage; cell division inhibition protein (NCBI), at 1,647,633 to 1,647,821
dicB
b1576: ydfD - Qin prophage; predicted protein (NCBI), at 1,647,818 to 1,648,009
ydfD
b1577: ydfE - Qin prophage; predicted protein (RefSeq), at 1,648,102 to 1,648,866
ydfE
b1578: insD - Qin prophage; IS2 insertion element transposase InsAB', C-ter fragment (pseudogene) (RefSeq), at 1,648,869 to 1,649,561
insD
Position (kb)
1647
1648
1649 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1646.887 kb on - strand, within ydfC at 1646.997 kb on - strand, within ydfC at 1647.366 kb on - strand at 1647.500 kb on - strand at 1647.653 kb on + strand, within dicB at 1647.700 kb on + strand, within dicB at 1647.709 kb on + strand, within dicB at 1647.775 kb on - strand, within dicB at 1647.880 kb on - strand, within ydfD at 1647.900 kb on - strand, within ydfD at 1647.957 kb on - strand, within ydfD at 1648.158 kb on - strand at 1648.158 kb on - strand at 1648.173 kb on + strand at 1648.173 kb on + strand at 1648.176 kb on - strand at 1648.176 kb on - strand at 1648.191 kb on - strand, within ydfE at 1648.250 kb on - strand, within ydfE at 1648.252 kb on + strand, within ydfE at 1648.260 kb on - strand, within ydfE at 1648.260 kb on - strand, within ydfE at 1648.260 kb on - strand, within ydfE at 1648.260 kb on - strand, within ydfE at 1648.297 kb on + strand, within ydfE at 1648.301 kb on - strand, within ydfE at 1648.301 kb on - strand, within ydfE at 1648.305 kb on - strand, within ydfE at 1648.306 kb on + strand, within ydfE at 1648.333 kb on + strand, within ydfE at 1648.357 kb on + strand, within ydfE at 1648.391 kb on - strand, within ydfE at 1648.391 kb on - strand, within ydfE at 1648.446 kb on - strand, within ydfE at 1648.725 kb on + strand, within ydfE
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM remove 1,646,887 - ydfC b1573 0.18 +0.5 1,646,997 - ydfC b1573 0.68 +0.5 1,647,366 - +0.7 1,647,500 - -0.9 1,647,653 + dicB b1575 0.11 +1.8 1,647,700 + dicB b1575 0.35 -0.6 1,647,709 + dicB b1575 0.40 +0.5 1,647,775 - dicB b1575 0.75 -1.1 1,647,880 - ydfD b1576 0.32 -1.6 1,647,900 - ydfD b1576 0.43 -1.4 1,647,957 - ydfD b1576 0.72 -1.6 1,648,158 - -0.2 1,648,158 - +1.2 1,648,173 + -2.6 1,648,173 + -0.8 1,648,176 - +1.0 1,648,176 - -0.0 1,648,191 - ydfE b1577 0.12 +0.1 1,648,250 - ydfE b1577 0.19 +1.2 1,648,252 + ydfE b1577 0.20 -1.3 1,648,260 - ydfE b1577 0.21 +0.7 1,648,260 - ydfE b1577 0.21 -0.6 1,648,260 - ydfE b1577 0.21 +0.4 1,648,260 - ydfE b1577 0.21 +0.1 1,648,297 + ydfE b1577 0.25 -0.1 1,648,301 - ydfE b1577 0.26 +0.4 1,648,301 - ydfE b1577 0.26 -0.9 1,648,305 - ydfE b1577 0.27 +0.1 1,648,306 + ydfE b1577 0.27 -0.8 1,648,333 + ydfE b1577 0.30 -0.9 1,648,357 + ydfE b1577 0.33 -0.0 1,648,391 - ydfE b1577 0.38 +0.8 1,648,391 - ydfE b1577 0.38 -0.6 1,648,446 - ydfE b1577 0.45 -1.1 1,648,725 + ydfE b1577 0.81 +1.8
Or see this region's nucleotide sequence