Strain Fitness in Escherichia coli BW25113 around b1035

Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycdW and ycdX are separated by 54 nucleotidesycdX and ycdY are separated by 23 nucleotidesycdY and ycdZ are separated by 101 nucleotidesycdZ and csgG are separated by 63 nucleotides b1033: ycdW - putative dehydrogenase (VIMSS), at 1,097,109 to 1,098,047 ycdW b1034: ycdX - hypothetical protein (NCBI), at 1,098,102 to 1,098,839 ycdX b1035: ycdY - hypothetical protein (NCBI), at 1,098,863 to 1,099,417 ycdY b1036: ycdZ - orf, hypothetical protein (VIMSS), at 1,099,519 to 1,100,010 ycdZ b1037: csgG - outer membrane lipoprotein (NCBI), at 1,100,074 to 1,100,907 csgG Position (kb) 1098 1099 1100Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 1097.934 kb on - strand, within ycdWat 1097.941 kb on + strand, within ycdWat 1098.011 kb on + strandat 1098.107 kb on - strandat 1098.107 kb on - strandat 1098.140 kb on + strandat 1098.174 kb on + strandat 1098.250 kb on + strand, within ycdXat 1098.274 kb on - strand, within ycdXat 1098.295 kb on + strand, within ycdXat 1098.303 kb on - strand, within ycdXat 1098.304 kb on - strand, within ycdXat 1098.304 kb on - strand, within ycdXat 1098.390 kb on - strand, within ycdXat 1098.390 kb on - strand, within ycdXat 1098.447 kb on - strand, within ycdXat 1098.459 kb on + strand, within ycdXat 1098.465 kb on - strand, within ycdXat 1098.465 kb on - strand, within ycdXat 1098.656 kb on - strand, within ycdXat 1098.800 kb on + strandat 1098.805 kb on + strandat 1098.805 kb on + strandat 1098.810 kb on + strandat 1098.813 kb on - strandat 1098.813 kb on - strandat 1098.826 kb on + strandat 1098.888 kb on - strandat 1098.892 kb on + strandat 1098.924 kb on + strand, within ycdYat 1098.998 kb on + strand, within ycdYat 1099.046 kb on + strand, within ycdYat 1099.106 kb on - strand, within ycdYat 1099.106 kb on - strand, within ycdYat 1099.189 kb on + strand, within ycdYat 1099.270 kb on - strand, within ycdYat 1099.290 kb on + strand, within ycdYat 1099.293 kb on - strand, within ycdYat 1099.293 kb on - strand, within ycdYat 1099.313 kb on + strand, within ycdYat 1099.313 kb on + strand, within ycdYat 1099.355 kb on - strand, within ycdYat 1099.588 kb on + strand, within ycdZat 1099.623 kb on - strand, within ycdZat 1099.702 kb on + strand, within ycdZat 1099.702 kb on + strand, within ycdZat 1099.710 kb on - strand, within ycdZat 1099.727 kb on + strand, within ycdZat 1099.758 kb on + strand, within ycdZat 1099.812 kb on - strand, within ycdZat 1099.880 kb on - strand, within ycdZat 1099.962 kb on + strandat 1099.987 kb on - strandat 1099.987 kb on - strandat 1100.096 kb on - strandat 1100.152 kb on + strandat 1100.152 kb on + strandat 1100.172 kb on - strand, within csgGat 1100.172 kb on - strand, within csgGat 1100.172 kb on - strand, within csgGat 1100.221 kb on - strand, within csgGat 1100.223 kb on + strand, within csgGat 1100.223 kb on + strand, within csgGat 1100.235 kb on + strand, within csgGat 1100.249 kb on - strand, within csgGat 1100.251 kb on - strand, within csgGat 1100.265 kb on - strand, within csgGat 1100.363 kb on - strand, within csgGat 1100.410 kb on - strand, within csgGat 1100.410 kb on - strand, within csgG

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM
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1,097,934 - ycdW b1033 0.88 -0.1
1,097,941 + ycdW b1033 0.89 +1.3
1,098,011 + +1.2
1,098,107 - -0.1
1,098,107 - +0.6
1,098,140 + +2.4
1,098,174 + +2.2
1,098,250 + ycdX b1034 0.20 +0.6
1,098,274 - ycdX b1034 0.23 +0.2
1,098,295 + ycdX b1034 0.26 +1.1
1,098,303 - ycdX b1034 0.27 +0.6
1,098,304 - ycdX b1034 0.27 +1.3
1,098,304 - ycdX b1034 0.27 +0.8
1,098,390 - ycdX b1034 0.39 -0.3
1,098,390 - ycdX b1034 0.39 +0.9
1,098,447 - ycdX b1034 0.47 +0.6
1,098,459 + ycdX b1034 0.48 +1.7
1,098,465 - ycdX b1034 0.49 +0.8
1,098,465 - ycdX b1034 0.49 +0.6
1,098,656 - ycdX b1034 0.75 -0.1
1,098,800 + +1.9
1,098,805 + +1.1
1,098,805 + +1.1
1,098,810 + +0.8
1,098,813 - +0.6
1,098,813 - +2.2
1,098,826 + +1.3
1,098,888 - +2.6
1,098,892 + +1.1
1,098,924 + ycdY b1035 0.11 +1.5
1,098,998 + ycdY b1035 0.24 +0.4
1,099,046 + ycdY b1035 0.33 +0.9
1,099,106 - ycdY b1035 0.44 -0.0
1,099,106 - ycdY b1035 0.44 +0.6
1,099,189 + ycdY b1035 0.59 +1.3
1,099,270 - ycdY b1035 0.73 -0.7
1,099,290 + ycdY b1035 0.77 +1.8
1,099,293 - ycdY b1035 0.77 -0.5
1,099,293 - ycdY b1035 0.77 +0.3
1,099,313 + ycdY b1035 0.81 +2.9
1,099,313 + ycdY b1035 0.81 +2.0
1,099,355 - ycdY b1035 0.89 +0.2
1,099,588 + ycdZ b1036 0.14 +4.3
1,099,623 - ycdZ b1036 0.21 +5.7
1,099,702 + ycdZ b1036 0.37 +4.4
1,099,702 + ycdZ b1036 0.37 +4.1
1,099,710 - ycdZ b1036 0.39 +4.8
1,099,727 + ycdZ b1036 0.42 +3.7
1,099,758 + ycdZ b1036 0.49 +4.8
1,099,812 - ycdZ b1036 0.60 +4.3
1,099,880 - ycdZ b1036 0.73 +4.6
1,099,962 + +4.6
1,099,987 - +3.6
1,099,987 - +4.5
1,100,096 - -0.5
1,100,152 + +0.1
1,100,152 + +1.0
1,100,172 - csgG b1037 0.12 +0.1
1,100,172 - csgG b1037 0.12 +0.7
1,100,172 - csgG b1037 0.12 +0.4
1,100,221 - csgG b1037 0.18 +0.8
1,100,223 + csgG b1037 0.18 +0.6
1,100,223 + csgG b1037 0.18 +0.1
1,100,235 + csgG b1037 0.19 +0.1
1,100,249 - csgG b1037 0.21 +0.2
1,100,251 - csgG b1037 0.21 +0.3
1,100,265 - csgG b1037 0.23 +0.5
1,100,363 - csgG b1037 0.35 +1.6
1,100,410 - csgG b1037 0.40 -0.4
1,100,410 - csgG b1037 0.40 +2.5

Or see this region's nucleotide sequence