Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybjP and ybjQ are separated by 125 nucleotides ybjQ and ybjR overlap by 4 nucleotides ybjR and b0868 overlap by 4 nucleotides
b0865: ybjP - predicted lipoprotein (NCBI), at 903,175 to 903,690
ybjP
b0866: ybjQ - hypothetical protein (NCBI), at 903,816 to 904,139
ybjQ
b0867: ybjR - predicted amidase and lipoprotein (NCBI), at 904,136 to 904,966
ybjR
b0868: b0868 - putative nucleotide di-P-sugar epimerase or dehydratase (VIMSS), at 904,963 to 905,976
b0868
Position (kb) 904
905 Strain fitness (log2 ratio) -2
-1
0
1 at 903.275 kb on + strand, within ybjP at 903.299 kb on + strand, within ybjP at 903.473 kb on + strand, within ybjP at 903.473 kb on + strand, within ybjP at 903.597 kb on - strand, within ybjP at 903.597 kb on - strand, within ybjP at 903.641 kb on + strand at 903.651 kb on + strand at 903.695 kb on - strand at 903.842 kb on + strand at 904.093 kb on + strand, within ybjQ at 904.156 kb on + strand at 904.183 kb on + strand at 904.271 kb on - strand, within ybjR at 904.282 kb on + strand, within ybjR at 904.298 kb on + strand, within ybjR at 904.352 kb on - strand, within ybjR at 904.465 kb on + strand, within ybjR at 904.477 kb on - strand, within ybjR at 904.477 kb on - strand, within ybjR at 904.479 kb on + strand, within ybjR at 904.558 kb on + strand, within ybjR at 904.597 kb on - strand, within ybjR at 904.765 kb on - strand, within ybjR at 904.775 kb on - strand, within ybjR at 904.812 kb on - strand, within ybjR at 904.812 kb on - strand, within ybjR at 904.812 kb on - strand, within ybjR at 904.824 kb on + strand, within ybjR at 904.830 kb on + strand, within ybjR at 904.830 kb on + strand, within ybjR at 904.838 kb on - strand, within ybjR at 904.935 kb on + strand at 904.968 kb on - strand at 905.027 kb on - strand at 905.245 kb on + strand, within b0868 at 905.246 kb on - strand, within b0868 at 905.246 kb on - strand, within b0868 at 905.248 kb on + strand, within b0868 at 905.253 kb on + strand, within b0868 at 905.261 kb on - strand, within b0868 at 905.261 kb on - strand, within b0868 at 905.275 kb on - strand, within b0868 at 905.278 kb on + strand, within b0868 at 905.278 kb on + strand, within b0868 at 905.284 kb on - strand, within b0868 at 905.286 kb on - strand, within b0868 at 905.357 kb on - strand, within b0868 at 905.357 kb on - strand, within b0868 at 905.376 kb on - strand, within b0868 at 905.402 kb on + strand, within b0868 at 905.468 kb on + strand, within b0868 at 905.489 kb on - strand, within b0868 at 905.525 kb on - strand, within b0868 at 905.543 kb on + strand, within b0868 at 905.734 kb on + strand, within b0868 at 905.734 kb on + strand, within b0868 at 905.735 kb on + strand, within b0868 at 905.735 kb on + strand, within b0868 at 905.746 kb on + strand, within b0868 at 905.964 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM remove 903,275 + ybjP b0865 0.19 +0.5 903,299 + ybjP b0865 0.24 +0.1 903,473 + ybjP b0865 0.58 -0.3 903,473 + ybjP b0865 0.58 +1.3 903,597 - ybjP b0865 0.82 -0.6 903,597 - ybjP b0865 0.82 +1.5 903,641 + +0.2 903,651 + -1.0 903,695 - +0.3 903,842 + +0.7 904,093 + ybjQ b0866 0.85 +0.1 904,156 + +0.4 904,183 + -0.3 904,271 - ybjR b0867 0.16 -0.5 904,282 + ybjR b0867 0.18 -0.2 904,298 + ybjR b0867 0.19 -0.4 904,352 - ybjR b0867 0.26 -0.1 904,465 + ybjR b0867 0.40 +1.1 904,477 - ybjR b0867 0.41 -0.3 904,477 - ybjR b0867 0.41 -1.9 904,479 + ybjR b0867 0.41 +0.2 904,558 + ybjR b0867 0.51 +0.0 904,597 - ybjR b0867 0.55 -0.6 904,765 - ybjR b0867 0.76 +0.1 904,775 - ybjR b0867 0.77 -0.0 904,812 - ybjR b0867 0.81 -0.0 904,812 - ybjR b0867 0.81 +0.5 904,812 - ybjR b0867 0.81 -0.3 904,824 + ybjR b0867 0.83 -0.6 904,830 + ybjR b0867 0.84 +0.4 904,830 + ybjR b0867 0.84 -0.3 904,838 - ybjR b0867 0.84 +0.5 904,935 + +0.5 904,968 - +0.5 905,027 - -0.2 905,245 + b0868 b0868 0.28 -0.4 905,246 - b0868 b0868 0.28 +0.8 905,246 - b0868 b0868 0.28 +1.1 905,248 + b0868 b0868 0.28 -1.0 905,253 + b0868 b0868 0.29 +0.8 905,261 - b0868 b0868 0.29 +1.1 905,261 - b0868 b0868 0.29 -0.1 905,275 - b0868 b0868 0.31 +0.1 905,278 + b0868 b0868 0.31 +0.3 905,278 + b0868 b0868 0.31 -0.2 905,284 - b0868 b0868 0.32 -0.7 905,286 - b0868 b0868 0.32 +0.9 905,357 - b0868 b0868 0.39 -0.7 905,357 - b0868 b0868 0.39 -0.4 905,376 - b0868 b0868 0.41 -0.2 905,402 + b0868 b0868 0.43 +0.3 905,468 + b0868 b0868 0.50 -2.1 905,489 - b0868 b0868 0.52 -0.3 905,525 - b0868 b0868 0.55 +0.2 905,543 + b0868 b0868 0.57 -0.9 905,734 + b0868 b0868 0.76 +0.5 905,734 + b0868 b0868 0.76 +0.5 905,735 + b0868 b0868 0.76 +1.1 905,735 + b0868 b0868 0.76 +1.1 905,746 + b0868 b0868 0.77 -0.0 905,964 + -0.2
Or see this region's nucleotide sequence