Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_58 and PfGW456L13_59 are separated by 127 nucleotides PfGW456L13_59 and PfGW456L13_60 are separated by 65 nucleotides PfGW456L13_60 and PfGW456L13_61 are separated by 187 nucleotides
PfGW456L13_58 - Phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 68,061 to 69,356
_58
PfGW456L13_59 - Sensor histidine kinase/response regulator, at 69,484 to 72,234
_59
PfGW456L13_60 - membrane protein, MarC family, at 72,300 to 72,896
_60
PfGW456L13_61 - hypothetical protein, at 73,084 to 73,407
_61
Position (kb)
69
70
71
72
73 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 69.355 kb on - strand at 69.355 kb on - strand at 69.355 kb on - strand at 69.355 kb on - strand at 69.359 kb on + strand at 69.359 kb on + strand at 69.359 kb on + strand at 69.360 kb on - strand at 69.389 kb on + strand at 69.390 kb on - strand at 69.400 kb on + strand at 69.421 kb on + strand at 69.421 kb on + strand at 69.422 kb on - strand at 69.422 kb on - strand at 69.422 kb on - strand at 69.423 kb on + strand at 69.423 kb on + strand at 69.423 kb on + strand at 69.424 kb on - strand at 69.424 kb on - strand at 69.424 kb on - strand at 69.424 kb on - strand at 69.439 kb on + strand at 69.439 kb on + strand at 69.440 kb on - strand at 69.479 kb on + strand at 69.479 kb on + strand at 69.480 kb on - strand at 69.519 kb on - strand at 69.607 kb on + strand at 69.607 kb on + strand at 69.608 kb on - strand at 69.754 kb on + strand at 69.754 kb on + strand at 69.754 kb on + strand at 69.755 kb on - strand at 69.881 kb on + strand, within PfGW456L13_59 at 69.882 kb on - strand, within PfGW456L13_59 at 69.882 kb on - strand, within PfGW456L13_59 at 69.882 kb on - strand, within PfGW456L13_59 at 69.882 kb on - strand, within PfGW456L13_59 at 69.919 kb on + strand, within PfGW456L13_59 at 69.919 kb on + strand, within PfGW456L13_59 at 69.920 kb on - strand, within PfGW456L13_59 at 69.920 kb on - strand, within PfGW456L13_59 at 69.920 kb on - strand, within PfGW456L13_59 at 69.920 kb on - strand, within PfGW456L13_59 at 69.976 kb on + strand, within PfGW456L13_59 at 69.976 kb on + strand, within PfGW456L13_59 at 69.977 kb on - strand, within PfGW456L13_59 at 70.030 kb on + strand at 70.179 kb on - strand, within PfGW456L13_59 at 70.179 kb on - strand, within PfGW456L13_59 at 70.179 kb on - strand, within PfGW456L13_59 at 70.311 kb on + strand, within PfGW456L13_59 at 70.336 kb on + strand, within PfGW456L13_59 at 70.337 kb on - strand, within PfGW456L13_59 at 70.346 kb on + strand, within PfGW456L13_59 at 70.759 kb on + strand, within PfGW456L13_59 at 70.764 kb on - strand, within PfGW456L13_59 at 71.522 kb on - strand, within PfGW456L13_59 at 71.531 kb on + strand, within PfGW456L13_59 at 71.531 kb on + strand, within PfGW456L13_59 at 71.531 kb on + strand, within PfGW456L13_59 at 71.531 kb on + strand, within PfGW456L13_59 at 71.531 kb on + strand, within PfGW456L13_59 at 71.532 kb on - strand, within PfGW456L13_59 at 71.532 kb on - strand, within PfGW456L13_59 at 71.532 kb on - strand, within PfGW456L13_59 at 72.085 kb on - strand at 72.100 kb on - strand at 72.259 kb on + strand at 72.259 kb on + strand at 72.259 kb on + strand at 72.260 kb on - strand at 72.325 kb on - strand at 72.346 kb on - strand at 72.393 kb on + strand, within PfGW456L13_60 at 72.430 kb on + strand, within PfGW456L13_60 at 72.465 kb on + strand, within PfGW456L13_60 at 72.465 kb on + strand, within PfGW456L13_60 at 72.465 kb on + strand, within PfGW456L13_60 at 72.465 kb on + strand, within PfGW456L13_60 at 72.466 kb on - strand, within PfGW456L13_60 at 72.466 kb on - strand, within PfGW456L13_60 at 72.466 kb on - strand, within PfGW456L13_60 at 72.504 kb on - strand, within PfGW456L13_60 at 72.743 kb on + strand, within PfGW456L13_60 at 72.743 kb on + strand, within PfGW456L13_60 at 72.744 kb on - strand, within PfGW456L13_60 at 73.028 kb on - strand at 73.061 kb on + strand at 73.061 kb on + strand at 73.061 kb on + strand at 73.061 kb on + strand at 73.062 kb on - strand at 73.062 kb on - strand at 73.062 kb on - strand at 73.062 kb on - strand at 73.156 kb on + strand, within PfGW456L13_61 at 73.157 kb on - strand, within PfGW456L13_61
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 69,355 - -0.1 69,355 - -0.1 69,355 - +0.8 69,355 - +0.3 69,359 + -0.2 69,359 + +1.2 69,359 + +0.1 69,360 - -0.2 69,389 + +0.4 69,390 - +0.6 69,400 + -0.3 69,421 + +1.6 69,421 + +0.4 69,422 - -0.1 69,422 - +0.5 69,422 - +0.2 69,423 + -0.6 69,423 + +1.1 69,423 + +0.3 69,424 - -2.1 69,424 - -0.1 69,424 - -0.3 69,424 - -0.2 69,439 + -0.4 69,439 + +2.1 69,440 - +0.1 69,479 + -2.5 69,479 + +0.4 69,480 - -0.2 69,519 - +0.2 69,607 + -0.9 69,607 + +0.1 69,608 - -0.1 69,754 + -0.1 69,754 + -2.1 69,754 + -1.2 69,755 - -1.2 69,881 + PfGW456L13_59 0.14 +0.1 69,882 - PfGW456L13_59 0.14 -2.8 69,882 - PfGW456L13_59 0.14 -1.2 69,882 - PfGW456L13_59 0.14 -1.4 69,882 - PfGW456L13_59 0.14 -1.0 69,919 + PfGW456L13_59 0.16 -1.7 69,919 + PfGW456L13_59 0.16 -2.8 69,920 - PfGW456L13_59 0.16 -0.7 69,920 - PfGW456L13_59 0.16 -1.1 69,920 - PfGW456L13_59 0.16 -1.1 69,920 - PfGW456L13_59 0.16 -2.4 69,976 + PfGW456L13_59 0.18 -0.6 69,976 + PfGW456L13_59 0.18 -3.3 69,977 - PfGW456L13_59 0.18 +0.1 70,030 + -0.2 70,179 - PfGW456L13_59 0.25 -2.6 70,179 - PfGW456L13_59 0.25 -1.1 70,179 - PfGW456L13_59 0.25 -1.5 70,311 + PfGW456L13_59 0.30 -2.5 70,336 + PfGW456L13_59 0.31 -2.5 70,337 - PfGW456L13_59 0.31 -1.5 70,346 + PfGW456L13_59 0.31 -2.7 70,759 + PfGW456L13_59 0.46 -2.7 70,764 - PfGW456L13_59 0.47 -0.9 71,522 - PfGW456L13_59 0.74 -2.4 71,531 + PfGW456L13_59 0.74 -1.5 71,531 + PfGW456L13_59 0.74 -0.9 71,531 + PfGW456L13_59 0.74 -1.1 71,531 + PfGW456L13_59 0.74 -1.9 71,531 + PfGW456L13_59 0.74 +0.7 71,532 - PfGW456L13_59 0.74 +0.5 71,532 - PfGW456L13_59 0.74 -0.7 71,532 - PfGW456L13_59 0.74 -1.2 72,085 - -3.0 72,100 - -1.6 72,259 + +0.2 72,259 + -0.1 72,259 + -0.6 72,260 - +0.8 72,325 - -0.4 72,346 - -0.5 72,393 + PfGW456L13_60 0.16 -0.1 72,430 + PfGW456L13_60 0.22 -0.3 72,465 + PfGW456L13_60 0.28 -0.4 72,465 + PfGW456L13_60 0.28 -0.1 72,465 + PfGW456L13_60 0.28 -0.1 72,465 + PfGW456L13_60 0.28 +1.3 72,466 - PfGW456L13_60 0.28 -0.3 72,466 - PfGW456L13_60 0.28 -0.3 72,466 - PfGW456L13_60 0.28 -0.7 72,504 - PfGW456L13_60 0.34 +1.2 72,743 + PfGW456L13_60 0.74 -0.3 72,743 + PfGW456L13_60 0.74 +0.1 72,744 - PfGW456L13_60 0.74 -0.5 73,028 - +1.2 73,061 + +1.8 73,061 + +1.3 73,061 + -1.1 73,061 + -0.2 73,062 - +0.0 73,062 - -1.3 73,062 - -2.7 73,062 - -1.4 73,156 + PfGW456L13_61 0.22 -0.0 73,157 - PfGW456L13_61 0.23 -0.1
Or see this region's nucleotide sequence