Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_50

Experiment: nitrate 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_49 and PfGW456L13_50 are separated by 132 nucleotidesPfGW456L13_50 and PfGW456L13_51 are separated by 24 nucleotidesPfGW456L13_51 and PfGW456L13_52 are separated by 17 nucleotides PfGW456L13_49 - Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8), at 58,067 to 59,812 _49 PfGW456L13_50 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 59,945 to 60,400 _50 PfGW456L13_51 - Biotin carboxyl carrier protein of acetyl-CoA carboxylase, at 60,425 to 60,889 _51 PfGW456L13_52 - Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14), at 60,907 to 62,265 _52 Position (kb) 59 60 61Strain fitness (log2 ratio) -3 -2 -1 0 1at 59.006 kb on + strand, within PfGW456L13_49at 59.006 kb on + strand, within PfGW456L13_49at 59.007 kb on - strand, within PfGW456L13_49at 59.007 kb on - strand, within PfGW456L13_49at 59.007 kb on - strand, within PfGW456L13_49at 59.007 kb on - strand, within PfGW456L13_49at 59.007 kb on - strand, within PfGW456L13_49at 59.007 kb on - strand, within PfGW456L13_49at 59.141 kb on + strand, within PfGW456L13_49at 59.141 kb on + strand, within PfGW456L13_49at 59.141 kb on + strand, within PfGW456L13_49at 59.141 kb on + strand, within PfGW456L13_49at 59.285 kb on + strand, within PfGW456L13_49at 59.285 kb on + strand, within PfGW456L13_49at 59.286 kb on - strand, within PfGW456L13_49at 59.286 kb on - strand, within PfGW456L13_49at 59.288 kb on - strand, within PfGW456L13_49at 59.339 kb on + strand, within PfGW456L13_49at 59.358 kb on - strand, within PfGW456L13_49at 59.534 kb on + strand, within PfGW456L13_49at 59.577 kb on + strand, within PfGW456L13_49at 59.578 kb on - strand, within PfGW456L13_49at 59.578 kb on - strand, within PfGW456L13_49at 59.678 kb on + strandat 59.787 kb on + strandat 59.787 kb on + strandat 59.787 kb on + strandat 59.788 kb on - strandat 59.788 kb on - strandat 59.810 kb on + strandat 59.826 kb on + strandat 59.826 kb on + strandat 59.826 kb on + strandat 59.827 kb on - strandat 59.827 kb on - strandat 59.928 kb on + strandat 59.928 kb on + strandat 59.928 kb on + strandat 59.928 kb on + strandat 59.929 kb on - strandat 59.933 kb on + strandat 59.933 kb on + strandat 59.933 kb on + strandat 59.933 kb on + strandat 59.933 kb on + strandat 59.933 kb on + strandat 59.933 kb on + strandat 59.934 kb on - strandat 59.934 kb on - strandat 59.934 kb on - strandat 59.934 kb on - strandat 59.934 kb on - strandat 60.011 kb on + strand, within PfGW456L13_50at 60.012 kb on - strand, within PfGW456L13_50at 60.012 kb on - strand, within PfGW456L13_50at 60.181 kb on + strand, within PfGW456L13_50at 60.181 kb on + strand, within PfGW456L13_50at 60.181 kb on + strand, within PfGW456L13_50at 60.181 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.187 kb on + strand, within PfGW456L13_50at 60.201 kb on + strand, within PfGW456L13_50at 60.201 kb on + strand, within PfGW456L13_50at 60.201 kb on + strand, within PfGW456L13_50at 60.201 kb on + strand, within PfGW456L13_50at 60.290 kb on + strand, within PfGW456L13_50at 60.290 kb on + strand, within PfGW456L13_50at 60.290 kb on + strand, within PfGW456L13_50at 60.290 kb on + strand, within PfGW456L13_50at 60.290 kb on + strand, within PfGW456L13_50

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 200 mM
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59,006 + PfGW456L13_49 0.54 -0.2
59,006 + PfGW456L13_49 0.54 +0.9
59,007 - PfGW456L13_49 0.54 -0.4
59,007 - PfGW456L13_49 0.54 -0.3
59,007 - PfGW456L13_49 0.54 +0.9
59,007 - PfGW456L13_49 0.54 +0.0
59,007 - PfGW456L13_49 0.54 +0.7
59,007 - PfGW456L13_49 0.54 +0.3
59,141 + PfGW456L13_49 0.62 +0.5
59,141 + PfGW456L13_49 0.62 -0.9
59,141 + PfGW456L13_49 0.62 +0.1
59,141 + PfGW456L13_49 0.62 -0.2
59,285 + PfGW456L13_49 0.70 -0.3
59,285 + PfGW456L13_49 0.70 -0.4
59,286 - PfGW456L13_49 0.70 +0.8
59,286 - PfGW456L13_49 0.70 -1.7
59,288 - PfGW456L13_49 0.70 -0.6
59,339 + PfGW456L13_49 0.73 -0.7
59,358 - PfGW456L13_49 0.74 +0.6
59,534 + PfGW456L13_49 0.84 +0.0
59,577 + PfGW456L13_49 0.86 +0.1
59,578 - PfGW456L13_49 0.87 -0.1
59,578 - PfGW456L13_49 0.87 +0.1
59,678 + -0.2
59,787 + -1.2
59,787 + -0.2
59,787 + -0.6
59,788 - +0.5
59,788 - +0.3
59,810 + +0.6
59,826 + -0.0
59,826 + +0.5
59,826 + -1.2
59,827 - -0.3
59,827 - +0.5
59,928 + -0.7
59,928 + -0.8
59,928 + -0.5
59,928 + -0.4
59,929 - -1.3
59,933 + +1.0
59,933 + -0.0
59,933 + +0.5
59,933 + +0.4
59,933 + -0.6
59,933 + +0.6
59,933 + -0.3
59,934 - -0.7
59,934 - -2.7
59,934 - -1.6
59,934 - -0.2
59,934 - -0.1
60,011 + PfGW456L13_50 0.14 -0.9
60,012 - PfGW456L13_50 0.15 -0.2
60,012 - PfGW456L13_50 0.15 -0.3
60,181 + PfGW456L13_50 0.52 -2.1
60,181 + PfGW456L13_50 0.52 -0.6
60,181 + PfGW456L13_50 0.52 -0.2
60,181 + PfGW456L13_50 0.52 -3.1
60,187 + PfGW456L13_50 0.53 -2.8
60,187 + PfGW456L13_50 0.53 -3.1
60,187 + PfGW456L13_50 0.53 -2.2
60,187 + PfGW456L13_50 0.53 -0.7
60,187 + PfGW456L13_50 0.53 -2.5
60,187 + PfGW456L13_50 0.53 -1.5
60,187 + PfGW456L13_50 0.53 -1.1
60,187 + PfGW456L13_50 0.53 -1.9
60,187 + PfGW456L13_50 0.53 -2.1
60,187 + PfGW456L13_50 0.53 -3.0
60,187 + PfGW456L13_50 0.53 +1.1
60,187 + PfGW456L13_50 0.53 -3.1
60,187 + PfGW456L13_50 0.53 -3.5
60,187 + PfGW456L13_50 0.53 -2.4
60,187 + PfGW456L13_50 0.53 -1.6
60,201 + PfGW456L13_50 0.56 -0.3
60,201 + PfGW456L13_50 0.56 -2.9
60,201 + PfGW456L13_50 0.56 -3.4
60,201 + PfGW456L13_50 0.56 -0.6
60,290 + PfGW456L13_50 0.76 -1.8
60,290 + PfGW456L13_50 0.76 -1.0
60,290 + PfGW456L13_50 0.76 -1.7
60,290 + PfGW456L13_50 0.76 -1.5
60,290 + PfGW456L13_50 0.76 -1.6

Or see this region's nucleotide sequence