Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_275 and PfGW456L13_276 are separated by 151 nucleotides PfGW456L13_276 and PfGW456L13_277 are separated by 31 nucleotides
PfGW456L13_275 - 3-dehydroquinate synthase (EC 4.2.3.4), at 322,813 to 323,913
_275
PfGW456L13_276 - Shikimate kinase I (EC 2.7.1.71), at 324,065 to 324,556
_276
PfGW456L13_277 - Type IV pilus biogenesis protein PilQ, at 324,588 to 326,657
_277
Position (kb) 324
325 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 323.144 kb on + strand, within PfGW456L13_275 at 323.270 kb on + strand, within PfGW456L13_275 at 323.804 kb on - strand at 323.944 kb on + strand at 323.964 kb on + strand at 324.067 kb on - strand at 324.095 kb on + strand at 324.095 kb on + strand at 324.096 kb on - strand at 324.096 kb on - strand at 324.096 kb on - strand at 324.107 kb on + strand at 324.107 kb on + strand at 324.288 kb on + strand, within PfGW456L13_276 at 324.289 kb on - strand, within PfGW456L13_276 at 324.623 kb on + strand at 324.624 kb on - strand at 324.639 kb on + strand at 324.639 kb on + strand at 324.640 kb on - strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.725 kb on + strand at 324.726 kb on - strand at 324.726 kb on - strand at 324.726 kb on - strand at 324.726 kb on - strand at 324.726 kb on - strand at 324.726 kb on - strand at 324.726 kb on - strand at 324.726 kb on - strand at 324.729 kb on + strand at 324.729 kb on + strand at 324.729 kb on + strand at 324.729 kb on + strand at 324.729 kb on + strand at 324.729 kb on + strand at 324.730 kb on - strand at 324.730 kb on - strand at 324.730 kb on - strand at 324.730 kb on - strand at 324.730 kb on - strand at 324.746 kb on + strand at 324.831 kb on + strand, within PfGW456L13_277 at 324.832 kb on - strand, within PfGW456L13_277 at 324.832 kb on - strand, within PfGW456L13_277 at 325.193 kb on + strand, within PfGW456L13_277 at 325.193 kb on + strand, within PfGW456L13_277 at 325.309 kb on + strand, within PfGW456L13_277 at 325.310 kb on - strand, within PfGW456L13_277 at 325.310 kb on - strand, within PfGW456L13_277 at 325.310 kb on - strand, within PfGW456L13_277 at 325.418 kb on - strand, within PfGW456L13_277 at 325.418 kb on - strand, within PfGW456L13_277 at 325.531 kb on + strand, within PfGW456L13_277 at 325.531 kb on + strand, within PfGW456L13_277 at 325.531 kb on + strand, within PfGW456L13_277 at 325.532 kb on - strand, within PfGW456L13_277
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 323,144 + PfGW456L13_275 0.30 +0.8 323,270 + PfGW456L13_275 0.42 -0.2 323,804 - -1.5 323,944 + -0.1 323,964 + -0.9 324,067 - -1.0 324,095 + -0.8 324,095 + -0.6 324,096 - -0.7 324,096 - -1.1 324,096 - -0.9 324,107 + -0.6 324,107 + -0.7 324,288 + PfGW456L13_276 0.45 -3.0 324,289 - PfGW456L13_276 0.46 -1.7 324,623 + -0.6 324,624 - +0.6 324,639 + -0.4 324,639 + -0.8 324,640 - +0.6 324,725 + +0.1 324,725 + -1.0 324,725 + -0.1 324,725 + +0.8 324,725 + -1.2 324,725 + -0.2 324,725 + +1.1 324,725 + -0.3 324,725 + -0.2 324,725 + -0.6 324,725 + +0.1 324,725 + +0.6 324,725 + -0.3 324,725 + -0.9 324,726 - +0.2 324,726 - +0.2 324,726 - -0.1 324,726 - +0.4 324,726 - +0.9 324,726 - -0.3 324,726 - +0.3 324,726 - -1.7 324,729 + -1.3 324,729 + -2.5 324,729 + -0.2 324,729 + -0.0 324,729 + +0.3 324,729 + +1.0 324,730 - -1.7 324,730 - -1.0 324,730 - -0.5 324,730 - +0.5 324,730 - -0.2 324,746 + -1.0 324,831 + PfGW456L13_277 0.12 -0.3 324,832 - PfGW456L13_277 0.12 +1.2 324,832 - PfGW456L13_277 0.12 +2.9 325,193 + PfGW456L13_277 0.29 +0.4 325,193 + PfGW456L13_277 0.29 -0.4 325,309 + PfGW456L13_277 0.35 +0.3 325,310 - PfGW456L13_277 0.35 -1.1 325,310 - PfGW456L13_277 0.35 +0.5 325,310 - PfGW456L13_277 0.35 -0.5 325,418 - PfGW456L13_277 0.40 +0.7 325,418 - PfGW456L13_277 0.40 +1.0 325,531 + PfGW456L13_277 0.46 +0.1 325,531 + PfGW456L13_277 0.46 -0.3 325,531 + PfGW456L13_277 0.46 +0.3 325,532 - PfGW456L13_277 0.46 -0.2
Or see this region's nucleotide sequence