Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_272 and PfGW456L13_273 overlap by 21 nucleotides PfGW456L13_273 and PfGW456L13_274 are separated by 13 nucleotides PfGW456L13_274 and PfGW456L13_275 are separated by 10 nucleotides
PfGW456L13_272 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 316,406 to 320,851
_272
PfGW456L13_273 - hypothetical protein, at 320,831 to 321,187
_273
PfGW456L13_274 - DamX, an inner membrane protein involved in bile resistance, at 321,201 to 322,802
_274
PfGW456L13_275 - 3-dehydroquinate synthase (EC 4.2.3.4), at 322,813 to 323,913
_275
Position (kb) 321
322
323 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 320.247 kb on + strand, within PfGW456L13_272 at 320.248 kb on - strand, within PfGW456L13_272 at 320.287 kb on - strand, within PfGW456L13_272 at 320.319 kb on + strand, within PfGW456L13_272 at 320.319 kb on + strand, within PfGW456L13_272 at 320.319 kb on + strand, within PfGW456L13_272 at 320.319 kb on + strand, within PfGW456L13_272 at 320.319 kb on + strand, within PfGW456L13_272 at 320.319 kb on + strand, within PfGW456L13_272 at 320.319 kb on + strand, within PfGW456L13_272 at 320.319 kb on + strand, within PfGW456L13_272 at 320.319 kb on + strand, within PfGW456L13_272 at 320.320 kb on - strand, within PfGW456L13_272 at 320.320 kb on - strand, within PfGW456L13_272 at 320.320 kb on - strand, within PfGW456L13_272 at 320.320 kb on - strand, within PfGW456L13_272 at 320.320 kb on - strand, within PfGW456L13_272 at 320.320 kb on - strand, within PfGW456L13_272 at 320.320 kb on - strand, within PfGW456L13_272 at 320.320 kb on - strand, within PfGW456L13_272 at 320.320 kb on - strand, within PfGW456L13_272 at 320.320 kb on - strand, within PfGW456L13_272 at 320.598 kb on + strand at 320.598 kb on + strand at 320.598 kb on + strand at 320.599 kb on - strand at 320.599 kb on - strand at 320.599 kb on - strand at 320.708 kb on - strand at 320.764 kb on + strand at 320.765 kb on - strand at 320.786 kb on - strand at 320.786 kb on - strand at 320.812 kb on + strand at 320.812 kb on + strand at 320.812 kb on + strand at 320.813 kb on - strand at 320.813 kb on - strand at 320.813 kb on - strand at 320.813 kb on - strand at 320.813 kb on - strand at 320.836 kb on - strand at 320.929 kb on - strand, within PfGW456L13_273 at 320.964 kb on + strand, within PfGW456L13_273 at 320.964 kb on + strand, within PfGW456L13_273 at 320.964 kb on + strand, within PfGW456L13_273 at 320.964 kb on + strand, within PfGW456L13_273 at 320.964 kb on + strand, within PfGW456L13_273 at 320.965 kb on - strand, within PfGW456L13_273 at 320.965 kb on - strand, within PfGW456L13_273 at 320.965 kb on - strand, within PfGW456L13_273 at 320.965 kb on - strand, within PfGW456L13_273 at 321.097 kb on + strand, within PfGW456L13_273 at 321.097 kb on + strand at 321.097 kb on + strand, within PfGW456L13_273 at 321.257 kb on + strand at 321.258 kb on - strand at 321.329 kb on - strand at 321.329 kb on - strand at 321.340 kb on + strand at 321.340 kb on + strand at 321.340 kb on + strand at 321.340 kb on + strand at 321.340 kb on + strand at 321.340 kb on + strand at 321.340 kb on + strand at 321.341 kb on - strand at 321.341 kb on - strand at 321.341 kb on - strand at 321.373 kb on + strand, within PfGW456L13_274 at 321.374 kb on - strand, within PfGW456L13_274 at 321.374 kb on - strand, within PfGW456L13_274 at 321.451 kb on + strand, within PfGW456L13_274 at 321.451 kb on + strand, within PfGW456L13_274 at 321.451 kb on + strand, within PfGW456L13_274 at 321.451 kb on + strand, within PfGW456L13_274 at 321.451 kb on + strand, within PfGW456L13_274 at 321.451 kb on + strand, within PfGW456L13_274 at 321.451 kb on + strand, within PfGW456L13_274 at 321.451 kb on + strand, within PfGW456L13_274 at 321.451 kb on + strand, within PfGW456L13_274 at 321.451 kb on + strand, within PfGW456L13_274 at 321.451 kb on + strand, within PfGW456L13_274 at 321.451 kb on + strand, within PfGW456L13_274 at 321.452 kb on - strand, within PfGW456L13_274 at 321.452 kb on - strand, within PfGW456L13_274 at 321.452 kb on - strand, within PfGW456L13_274 at 321.452 kb on - strand, within PfGW456L13_274 at 321.452 kb on - strand, within PfGW456L13_274 at 321.452 kb on - strand, within PfGW456L13_274 at 321.475 kb on + strand, within PfGW456L13_274 at 321.475 kb on + strand, within PfGW456L13_274 at 321.475 kb on + strand, within PfGW456L13_274 at 321.476 kb on - strand, within PfGW456L13_274 at 321.476 kb on - strand, within PfGW456L13_274 at 321.595 kb on - strand, within PfGW456L13_274 at 321.978 kb on + strand, within PfGW456L13_274 at 322.028 kb on + strand, within PfGW456L13_274 at 322.028 kb on + strand, within PfGW456L13_274 at 322.028 kb on + strand, within PfGW456L13_274 at 322.028 kb on + strand, within PfGW456L13_274 at 322.146 kb on - strand, within PfGW456L13_274 at 322.213 kb on + strand, within PfGW456L13_274 at 322.420 kb on + strand, within PfGW456L13_274 at 322.421 kb on - strand, within PfGW456L13_274 at 322.421 kb on - strand, within PfGW456L13_274 at 322.421 kb on - strand, within PfGW456L13_274 at 322.421 kb on - strand, within PfGW456L13_274 at 322.518 kb on + strand, within PfGW456L13_274 at 322.518 kb on + strand, within PfGW456L13_274 at 322.518 kb on + strand, within PfGW456L13_274 at 322.566 kb on + strand, within PfGW456L13_274 at 322.661 kb on - strand at 322.762 kb on + strand at 322.763 kb on - strand at 322.796 kb on + strand at 322.796 kb on + strand at 322.796 kb on + strand at 322.796 kb on + strand at 322.796 kb on + strand at 322.796 kb on + strand at 322.797 kb on - strand at 322.797 kb on - strand at 322.797 kb on - strand at 322.797 kb on - strand at 322.797 kb on - strand at 322.797 kb on - strand at 322.797 kb on - strand at 322.811 kb on + strand at 322.811 kb on + strand at 322.811 kb on + strand at 322.812 kb on - strand at 322.812 kb on - strand at 322.812 kb on - strand at 322.812 kb on - strand at 322.812 kb on - strand at 322.812 kb on - strand at 322.812 kb on - strand at 322.814 kb on + strand at 322.822 kb on - strand at 322.822 kb on - strand at 322.842 kb on - strand at 322.842 kb on - strand at 322.965 kb on + strand, within PfGW456L13_275 at 322.965 kb on + strand, within PfGW456L13_275 at 322.965 kb on + strand, within PfGW456L13_275 at 323.144 kb on + strand, within PfGW456L13_275 at 323.270 kb on + strand, within PfGW456L13_275
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 320,247 + PfGW456L13_272 0.86 +0.4 320,248 - PfGW456L13_272 0.86 -0.4 320,287 - PfGW456L13_272 0.87 +0.4 320,319 + PfGW456L13_272 0.88 -1.1 320,319 + PfGW456L13_272 0.88 +0.1 320,319 + PfGW456L13_272 0.88 -0.2 320,319 + PfGW456L13_272 0.88 +0.5 320,319 + PfGW456L13_272 0.88 -0.2 320,319 + PfGW456L13_272 0.88 -0.2 320,319 + PfGW456L13_272 0.88 +0.1 320,319 + PfGW456L13_272 0.88 -1.1 320,319 + PfGW456L13_272 0.88 -0.8 320,320 - PfGW456L13_272 0.88 +0.1 320,320 - PfGW456L13_272 0.88 +0.0 320,320 - PfGW456L13_272 0.88 +0.4 320,320 - PfGW456L13_272 0.88 -0.4 320,320 - PfGW456L13_272 0.88 +0.9 320,320 - PfGW456L13_272 0.88 -0.6 320,320 - PfGW456L13_272 0.88 -0.2 320,320 - PfGW456L13_272 0.88 -0.3 320,320 - PfGW456L13_272 0.88 +0.9 320,320 - PfGW456L13_272 0.88 -0.7 320,598 + +0.1 320,598 + -0.7 320,598 + -0.8 320,599 - -0.3 320,599 - -2.4 320,599 - -0.9 320,708 - +0.2 320,764 + +0.8 320,765 - -0.4 320,786 - -0.1 320,786 - +0.4 320,812 + -0.6 320,812 + -0.1 320,812 + -0.3 320,813 - -0.5 320,813 - +0.2 320,813 - -0.3 320,813 - +0.2 320,813 - -0.7 320,836 - -0.5 320,929 - PfGW456L13_273 0.27 -1.1 320,964 + PfGW456L13_273 0.37 +0.7 320,964 + PfGW456L13_273 0.37 +0.8 320,964 + PfGW456L13_273 0.37 +0.5 320,964 + PfGW456L13_273 0.37 +0.1 320,964 + PfGW456L13_273 0.37 -0.8 320,965 - PfGW456L13_273 0.38 -1.0 320,965 - PfGW456L13_273 0.38 -0.7 320,965 - PfGW456L13_273 0.38 -0.1 320,965 - PfGW456L13_273 0.38 +2.4 321,097 + PfGW456L13_273 0.75 -0.1 321,097 + -0.5 321,097 + PfGW456L13_273 0.75 +0.5 321,257 + -2.7 321,258 - -0.8 321,329 - -0.6 321,329 - -0.2 321,340 + -0.4 321,340 + -0.6 321,340 + -1.2 321,340 + -1.2 321,340 + -1.6 321,340 + -2.3 321,340 + -0.9 321,341 - -1.0 321,341 - -0.9 321,341 - -2.3 321,373 + PfGW456L13_274 0.11 -2.3 321,374 - PfGW456L13_274 0.11 -0.8 321,374 - PfGW456L13_274 0.11 -2.7 321,451 + PfGW456L13_274 0.16 -0.6 321,451 + PfGW456L13_274 0.16 -0.2 321,451 + PfGW456L13_274 0.16 -1.3 321,451 + PfGW456L13_274 0.16 +0.8 321,451 + PfGW456L13_274 0.16 -1.4 321,451 + PfGW456L13_274 0.16 -1.9 321,451 + PfGW456L13_274 0.16 -1.0 321,451 + PfGW456L13_274 0.16 -1.8 321,451 + PfGW456L13_274 0.16 -0.5 321,451 + PfGW456L13_274 0.16 -3.8 321,451 + PfGW456L13_274 0.16 -0.8 321,451 + PfGW456L13_274 0.16 -1.3 321,452 - PfGW456L13_274 0.16 -1.3 321,452 - PfGW456L13_274 0.16 -1.6 321,452 - PfGW456L13_274 0.16 -3.0 321,452 - PfGW456L13_274 0.16 -1.6 321,452 - PfGW456L13_274 0.16 -1.5 321,452 - PfGW456L13_274 0.16 -1.1 321,475 + PfGW456L13_274 0.17 -1.0 321,475 + PfGW456L13_274 0.17 -1.9 321,475 + PfGW456L13_274 0.17 -1.4 321,476 - PfGW456L13_274 0.17 -0.9 321,476 - PfGW456L13_274 0.17 -0.2 321,595 - PfGW456L13_274 0.25 -0.7 321,978 + PfGW456L13_274 0.49 -0.9 322,028 + PfGW456L13_274 0.52 -0.9 322,028 + PfGW456L13_274 0.52 -1.1 322,028 + PfGW456L13_274 0.52 -0.4 322,028 + PfGW456L13_274 0.52 -0.8 322,146 - PfGW456L13_274 0.59 -0.9 322,213 + PfGW456L13_274 0.63 +0.5 322,420 + PfGW456L13_274 0.76 -2.4 322,421 - PfGW456L13_274 0.76 -0.9 322,421 - PfGW456L13_274 0.76 -0.9 322,421 - PfGW456L13_274 0.76 -0.6 322,421 - PfGW456L13_274 0.76 -1.4 322,518 + PfGW456L13_274 0.82 -1.3 322,518 + PfGW456L13_274 0.82 -0.8 322,518 + PfGW456L13_274 0.82 -0.6 322,566 + PfGW456L13_274 0.85 -0.9 322,661 - -1.8 322,762 + -1.2 322,763 - -1.8 322,796 + -0.2 322,796 + -0.5 322,796 + -0.7 322,796 + -0.2 322,796 + -1.7 322,796 + -1.8 322,797 - -0.8 322,797 - -0.9 322,797 - -1.1 322,797 - -0.8 322,797 - -1.2 322,797 - -0.5 322,797 - -0.9 322,811 + -0.2 322,811 + -0.5 322,811 + -1.1 322,812 - -0.3 322,812 - -0.5 322,812 - -0.3 322,812 - +0.3 322,812 - -0.1 322,812 - +0.1 322,812 - -0.8 322,814 + -0.6 322,822 - -0.6 322,822 - -2.0 322,842 - +0.0 322,842 - -1.2 322,965 + PfGW456L13_275 0.14 -2.6 322,965 + PfGW456L13_275 0.14 -1.7 322,965 + PfGW456L13_275 0.14 -0.9 323,144 + PfGW456L13_275 0.30 +0.8 323,270 + PfGW456L13_275 0.42 -0.2
Or see this region's nucleotide sequence